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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0257 [new locus tag: NWMN_RS01420 ]
  • pan locus tag?: SAUPAN001239000
  • symbol: nanA
  • pan gene symbol?: nanA
  • synonym:
  • product: N-acetylneuraminate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0257 [new locus tag: NWMN_RS01420 ]
  • symbol: nanA
  • product: N-acetylneuraminate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 313178..314059
  • length: 882
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAACAAAGATTTAAAAGGTTTATATGCAGCGTTACTCGTTCCTTTTGATGAAAATGGT
    CAAGTAAATGAACAAGGACTTAAACAAATTGCTCAAAATGCCATTGAAACTGAAGAATTA
    GACGGTCTTTATGTAAATGGTAGCTCAGGCGAAAACTTTTTATTAAATACAGAGCAGAAG
    AAGCAAGTTTTCAAGGTTGCCAAAGAGGCAGTTGGGGATAAAGTGAAATTGATTGCTCAA
    GTAGGTTCGTTAGATTTAAATGAAGCCATTGAACTTGGAAAATATGCCACAGAACTCGGT
    TATGATGCACTTTCTGCCGTAACACCATTCTACTATCCATTTACTTTTGAAGAAATTAGA
    GATTACTATTTCGATATTATCGAAGCAACTCAGAACAATATGATTATTTATGCAATACCA
    GATTTAACAGGTGTGAATATTTCTATTGAACAATTCAGCGAGCTATTTAATCATGAAAAA
    ATTGTTGGTGTTAAATATACAGCGCCAAACTTCTTCCTACTCGAACGTATTAGAAAAGCA
    TTCCCAGACAAATTAATCTTATCTGGCTTTGATGAAATGTTAGTTCAAGCTACGATTTCT
    GGCGTAGATGGTGCAATTGGTTCTACATATAACGTTAATGGTCGCCGTGCAAGAAAAATC
    TTCGACTTAGCACGACAAGGTCAAATTCAAGAAGCTTACCAACTGCAACATGATTCAAAC
    GACATTATCGAAACAGTGTTATCAATGGGGATTTATCCAACATTGAAAGAAATATTACGT
    CATCGTGGCATTGATGCAGGATTACCGAAAAGACCTTTCAAACCTTTTAACGAAGCACAT
    CGACAAACATTAGATCAACTCATTGCAAAATACGATTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

Protein[edit source | edit]

Protein Data Bank: 4AH7
Protein Data Bank: 4AHO
Protein Data Bank: 4AHP
Protein Data Bank: 4AHQ
Protein Data Bank: 4AMA
Protein Data Bank: 5A8G

General[edit source | edit]

  • locus tag: NWMN_0257 [new locus tag: NWMN_RS01420 ]
  • symbol: NanA
  • description: N-acetylneuraminate lyase
  • length: 293
  • theoretical pI: 4.70663
  • theoretical MW: 33042.3
  • GRAVY: -0.232423

Function[edit source | edit]

  • reaction:
    EC 4.1.3.3?  ExPASy
    N-acetylneuraminate lyaseN-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesN-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 367.8)
    MetabolismCentral intermediary metabolismAmino sugarsN-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 367.8)
    MetabolismAmino acid biosynthesisAspartate family4-hydroxy-tetrahydrodipicolinate synthase (TIGR00674; EC 4.3.3.7; HMM-score: 139.2)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02313; EC 4.1.2.-; HMM-score: 103.3)
    5-dehydro-4-deoxyglucarate dehydratase (TIGR03249; EC 4.2.1.41; HMM-score: 56.9)
  • TheSEED:  
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSialic Acid Metabolism N-acetylneuraminate lyase (EC 4.1.3.3) 
  • PFAM:
    TIM_barrel (CL0036) DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 282.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 31
    • Cmax: 0.111
    • Cmax_pos: 23
    • Smax: 0.273
    • Smax_pos: 22
    • Smean: 0.161
    • D: 0.153
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: NWMN_0259 (NanR*), CcpA
    NanR* (TF) important in N-acetylglucosamine utilization, Sialic acid utilization  RegPrecise
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]