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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0346 [new locus tag: NWMN_RS01965 ]
  • pan locus tag?: SAUPAN001898000
  • symbol: NWMN_0346
  • pan gene symbol?:
  • synonym:
  • product: acetyl-CoA acetyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0346 [new locus tag: NWMN_RS01965 ]
  • symbol: NWMN_0346
  • product: acetyl-CoA acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 395604..396785
  • length: 1182
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACGAGAGTCGTATTAGCAGCAGCATACAGGACACCTATTGGCGTTTTTGGAGGTGCG
    TTTAAAGACGTGCCAGCCTATGATTTAGGTGCGACTTTAATAGAACATATTATTAAAGAG
    ACGGGTTTGAATCCAAGTGAGATTGATGAAGTTATCATCGGTAACGTACTACAAGCAGGA
    CAAGGACAAAATCCAGCACGAATTGCTGCTATGAAAGGTGGCTTGCCAGAAACAGTACCT
    GCATTTACGGTGAATAAAGTATGTGGTTCTGGGTTAAAGTCGATTCAATTAGCATATCAA
    TCTATTGTGACTGGTGAAAATGACATCGTGCTAGCTGGCGGTATGGAGAATATGTCTCAA
    TCACCAATGCTTGTCAACAACAGTCGCTTTGGTTTTAAAATGGGACATCAATCAATGGTT
    GATAGCATGGTATATGATGGTTTAACAGATGTATTTAATCAATATCATATGGGTATTACT
    GCTGAAAATTTAGTAGAGCAATATGGTATTTCAAGAGAAGAACAAGATACATTTGCTGTA
    AACTCACAACAAAAAGCAGTACGTGCACAGCAAAATGGTGAATTTGATAGTGAAATAGTT
    CCAGTATCGATTCCTCAACGTAAAGGTGAACCAATCGTAGTCACTAAGGATGAAGGTGTA
    CGTGAAAATGTATCAGTCGAAAAATTAAGTCGATTAAGACCAGCTTTCAAAAAAGACGGT
    ACAGTTACAGCAGGTAATGCATCAGGAATCAATGATGGTGCTGCGATGATGTTAGTCATG
    TCAGAAGACAAAGCTAAAGAATTAAATATCGAACCATTGGCAGTGCTTGATGGCTTTGGA
    AGTCATGGTGTAGATCCTTCTATTATGGGTATTGCACCAGTTGGCGCTGTAGAAAAGGCT
    TTGAAACGTAGTAAAAAAGAATTAAGCGATATTGATGTATTTGAATTAAATGAAGCATTT
    GCAGCACAATCATTAGCTGTTGATCGTGAATTAAAATTACCTCCTGAAAAGGTGAATGTT
    AAAGGTGGCGCTATTGCATTAGGACATCCTATTGGTGCATCTGGTGCTAGAGTATTAGTG
    ACATTATTGCATCAACTGAATGATGAAGTTGAAACTGGTTTAACATCATTGTGTATTGGT
    GGCGGTCAAGCTATCGCTGCAGTTGTATCAAAGTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0346 [new locus tag: NWMN_RS01965 ]
  • symbol: NWMN_0346
  • description: acetyl-CoA acetyltransferase
  • length: 393
  • theoretical pI: 4.94056
  • theoretical MW: 41703.4
  • GRAVY: -0.0389313

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismOtheracetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 501.1)
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 391.9)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 333.6)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 190.9)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisbeta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 27.2)
    MetabolismFatty acid and phospholipid metabolismBiosynthesis3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 16.8)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsIsoleucine degradation 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    CarbohydratesFermentationAcetyl-CoA fermentation to Butyrate Acetyl-CoA acetyltransferase (EC 2.3.1.9) 
    FermentationButanol Biosynthesis Acetyl-CoA acetyltransferase (EC 2.3.1.9) 
    One-carbon MetabolismSerine-glyoxylate cycle 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    One-carbon MetabolismSerine-glyoxylate cycle Acetyl-CoA acetyltransferase (EC 2.3.1.9) 
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    Fatty Acids, Lipids, and IsoprenoidsFatty Acids, Lipids, and Isoprenoids - no subcategoryPolyhydroxybutyrate metabolism 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    Fatty Acids, Lipids, and Isoprenoids - no subcategoryPolyhydroxybutyrate metabolism Acetyl-CoA acetyltransferase (EC 2.3.1.9) 
    IsoprenoidsArchaeal lipids Acetyl-CoA acetyltransferase (EC 2.3.1.9) 
    IsoprenoidsIsoprenoid Biosynthesis Acetyl-CoA acetyltransferase (EC 2.3.1.9) 
    Butyrate metabolism cluster 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 363.3)
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 151.2)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 28.3)
    FAE1_CUT1_RppA; FAE1/Type III polyketide synthase-like protein (PF08392; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.182
    • Ymax_pos: 23
    • Cmax: 0.144
    • Cmax_pos: 23
    • Smax: 0.346
    • Smax_pos: 1
    • Smean: 0.233
    • D: 0.202
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLNPSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSIVTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGITAENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDEGVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVLDGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLPPEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKYK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_0346 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]