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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0445 [new locus tag: NWMN_RS02535 ]
  • pan locus tag?: SAUPAN002215000
  • symbol: tmk
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0445 [new locus tag: NWMN_RS02535 ]
  • symbol: tmk
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 508675..510012
  • length: 1338
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGCAACCTATTTTAAATAAATTAGAAAGTTTAAATCAAGAAGAAGCGATTTCTTTG
    CATGTTCCGGGTCATAAAAATATGACTATCGGTCATTTATCTCAATTATCAATGACAATG
    GATAAAACTGAAATACCTGGATTAGATGATTTACATCATCCTGAAGAAGTCATTTTGGAA
    AGTATGAAGCAGGTGGAGAAACATTCAGATTATGATGCTTATTTCTTAGTGAATGGCACC
    ACTTCAGGAATATTATCTGTCATCCAGTCTTTTTCACAGAAAAAAGGCGATATCTTAATG
    GCAAGAAATGTACATAAATCTGTGTTACATGCGCTCGATATTAGCCAACAAGAAGGGCAT
    TTTATTGAAACGCATCAAAGTCCGTTAACGAATCATTATAATAAAGTTAATTTAAGCCGT
    TTGAATAATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACA
    TTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGAC
    GAAGCACACGGCGCGCACTTTGGATTGCAAGGATTTCCAGATTCTACATTAAATTATCAA
    GCTGACTATGTTGTTCAATCTTTTCATAAAACGTTACCAGCTTTAACGATGGGCTCGGTA
    CTTTATATTCATAAAAATGCACCTTATAGAGAAACTATTATAGAATATCTAAGCTACTTC
    CAAACATCTAGTCCTTCGTATTTGATTATGGCTAGTTTAGAGTCAGCTGCCGAGTTCTAT
    AAAACATATGATAGTACCGTGTTTTTTGATAAGAGAGCGCAATTAATCGAATGTTTGGAG
    AAGAAGGGTTTTGAAATGCTTCAAGTTGATGATCCGTTGAAGTTGCTGATAAAATATGAA
    GGTTTTACAGGTCATGATATTCAAAATTGGTTTATGAATGCACATATCTATTTAGAATTA
    GCGGACGACTATCAAGCATTAGCGATATTGCCGTTATGGCATCATGATGATACGTATTTA
    TTTGATTCGCTTTTACGTAAAATTGAAGATATGATTTTACCGAAAAAATCAGTTTCTAAA
    GTTAAACAAACACAACTTTTAACAACTGAAGGTAACTATAAACCAAAACGCTTTGAATAT
    GTTACTTGGTGTGATTTGAAAAAGGCAAAAGGTAAAGTTCTGGCGCGACATATTGTCCCG
    TATCCGCCAGGGATTCCTATTATTTTCAAAGGAGAAACAATAACTGAAAATATGATAGAA
    TTGGTAAATGAATATCTGGAAACTGGAATGATAGTTGAAGGAATTAAAAATAATAAAATT
    TTAGTTGAGGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1338

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0445 [new locus tag: NWMN_RS02535 ]
  • symbol: Tmk
  • description: hypothetical protein
  • length: 445
  • theoretical pI: 6.02953
  • theoretical MW: 51021
  • GRAVY: -0.29618

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    ornithine decarboxylase SpeF (TIGR04301; EC 4.1.1.17; HMM-score: 29.9)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 29)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, SufS family (TIGR01979; HMM-score: 26.5)
    putative ornithine decarboxylase (TIGR04318; EC 4.1.1.-; HMM-score: 25.9)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 25.1)
    Unknown functionEnzymes of unknown specificitypyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 15.3)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 11.4)
  • TheSEED:  
    Arginine and Ornithine Degradation Arginine decarboxylase (EC 4.1.1.19) / Lysine decarboxylase (EC 4.1.1.18) 
    Lysine degradation Arginine decarboxylase (EC 4.1.1.19) / Lysine decarboxylase (EC 4.1.1.18) 
    Polyamine Metabolism Arginine decarboxylase (EC 4.1.1.19) / Lysine decarboxylase (EC 4.1.1.18) 
  • PFAM:
    PLP_aminotran (CL0061) OKR_DC_1; Orn/Lys/Arg decarboxylase, major domain (PF01276; HMM-score: 146.2)
    no clan definedOKR_DC_1_C; Orn/Lys/Arg decarboxylase, C-terminal domain (PF03711; HMM-score: 35)
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 26.4)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 18.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 18
    • Cmax: 0.11
    • Cmax_pos: 18
    • Smax: 0.181
    • Smax_pos: 13
    • Smean: 0.151
    • D: 0.14
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTEIPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMARNVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGETFNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMGSVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLIECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWHHDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGKVLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]