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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0527 [new locus tag: NWMN_RS03050 ]
  • pan locus tag?: SAUPAN002340000
  • symbol: NWMN_0527
  • pan gene symbol?:
  • synonym:
  • product: glycosyl transferase, group 1 family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0527 [new locus tag: NWMN_RS03050 ]
  • symbol: NWMN_0527
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 614434..615924
  • length: 1491
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAATTATTTTGTAGGTAATAGTTTAGGTGTTAATTTAACTGGGATAGAGAAAGCAATT
    ATTAATCGTTTAAATTTATTTAAAGAAATGGGAAGACCAGCGCAATGTGTATTTCTGTCA
    TGGAATAGATATTTATATAGAAATGCCCAAAATTATATTACTAGTTCTGACTATATTAAT
    ATGTATGATTTCTTCCAAGAAGCAACATATTTAGAACGCAATGAACCATTTGACTGGTTG
    TCGTATTGGACTGATGAATGTCATTATACTTTAAAACATGTTGAAAATAGTCATGACTTT
    AGAATATATGATCAAGAGCGTTTCTTAATGTATGCCCATTTTCAAGATCCTAAATATCGT
    ATATTAGATTATGTAAATCATTTTGATAGTCAAAGACGAAAAGTAAAACGTGATTTTTAT
    GATGTGCGTGGCTTTTTAAGTTGTAGTAGGATTCTTGTTGATAAACAACAAACATTATGT
    GAATTTTTCTATAATCCAGAAGGTGACACAAAATTAGAAAAGTATTTTTCTTACAAGGAT
    GGCAAACCAGAAGTACAAAAAATTATTGTTTACTATGCTAACAAACAATATTTCTTTAAT
    AATGAAACGGAATTAGGCGCATTTTTCATTAAACAACTATATCAACATGGGGATTTATTT
    TTCAGTGATCGTAATGTGTACACAGCACCTATTTTCAACTTAACGCCTGAGTCAATCCCA
    GTAGTAGCTGTGCTTCACAGTACACACATTAAAAATATTGATGCATTAGATTCATCGCCA
    TTTAAAAATGTATATAAAGCGATGTTTGAAAATTTATCTCGATATCGTGCAATTATCGTT
    TCTACTGAGCAGCAAAAATTAGATGTTGAAAAACGTATTAACCATACTATTCCAGTTGTT
    AATATTCCTGTCGGCTATAGTGAAACAATTGATACACCAGTCCAAACATTAGATCAGCGC
    AGTGTTAAATTAATTTCCGTTGCTCGTTATTCACCTGAAAAACAATTACATCAACAAATT
    GAATTAATTAAACGCTTAGTATCGTATGTCCCAAAAATAGAGCTTCATATGTATGGTTTT
    GGTTCTGAAAGTAAAAAATTAAATGAATTGATACAAAAATATGGATTGGAAAATCATGTT
    TACTTACGCGGCTTTTTAAGCAATCTAGACCAAGAATATAGCGATGCCTATTTGAGTTTA
    ATAACAAGTAACATGGAAGGTTTCTCTTTAGCGCTACTTGAATCTTTAGCACATGGTGTT
    CCAGTTATTAGTTATGATATTAAATATGGACCTAACGAACTGATTACTTCAGATTTCAAT
    GGTTATTTAATTACTAAAAATGATGAAGATGCCCTATTTGATAAAGTAAAATATGTGATT
    GATCATCCTGAAGTACAGCAACGACTGTCAAAAGGTAGTTTGGCAAAAGCACAACAATAT
    AGTAAAGCGTCGTTAATTAAACAATGGGACCAATTTGTTCGTCTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0527 [new locus tag: NWMN_RS03050 ]
  • symbol: NWMN_0527
  • description: glycosyl transferase, group 1 family protein
  • length: 496
  • theoretical pI: 6.88884
  • theoretical MW: 58417.9
  • GRAVY: -0.422177

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein modification and repairaccessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 130.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 78.1)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 64.3)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 61.3)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 27.6)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 27.4)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 25.3)
    Genetic information processingProtein fateProtein modification and repairaccessory Sec system glycosyltransferase GtfB (TIGR02919; EC 2.4.1.-; HMM-score: 24.7)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 24.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 22.9)
    Unknown functionEnzymes of unknown specificityglycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 20.4)
    colanic acid biosynthesis glycosyltransferase WcaC (TIGR04015; EC 2.4.-.-; HMM-score: 15.5)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 10.5)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsTeichoic and lipoteichoic acids biosynthesis Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52) 
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 112.1)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 72.2)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 23.9)
    NTF2 (CL0051) Ring_hydroxyl_B; Ring hydroxylating beta subunit (PF00866; HMM-score: 17)
    no clan definedETC_C1_NDUFA5; ETC complex I subunit conserved region (PF04716; HMM-score: 14.1)
    Dcc1; Sister chromatid cohesion protein Dcc1 (PF09724; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 20
    • Cmax: 0.127
    • Cmax_pos: 20
    • Smax: 0.176
    • Smax_pos: 18
    • Smean: 0.139
    • D: 0.137
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_0527 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]