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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0550 [new locus tag: SAUSA300_RS02935 ]
  • pan locus tag?: SAUPAN002340000
  • symbol: SAUSA300_0550
  • pan gene symbol?: —
  • synonym:
  • product: glycosyl transferase, group 1 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0550 [new locus tag: SAUSA300_RS02935 ]
  • symbol: SAUSA300_0550
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 624075..625565
  • length: 1491
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAATTATTTTGTAGGTAATAGTTTAGGTGTTAATTTAACTGGGATAGAGAAAGCAATT
    ATTAATCGTTTAAATTTATTTAAAGAAATGGGAAGACCAGCGCAATGTGTATTTCTGTCA
    TGGAATAGATATTTATATAGAAATGCCCAAAATTATATTACTAGTTCTGACTATATTAAT
    ATGTATGATTTCTTCCAAGAAGCAACATATTTAGAACGCAATGAACCATTTGACTGGTTG
    TCGTATTGGACTGATGAATGTCATTATACTTTAAAACATGTTGAAAATAGTCATGACTTT
    AGAATATATGATCAAGAGCGTTTCTTAATGTATGCCCATTTTCAAGATCCTAAATATCGT
    ATATTAGATTATGTAAATCATTTTGATAGTCAAAGACGAAAAGTAAAACGTGATTTTTAT
    GATGTGCGTGGCTTTTTAAGTTGTAGTAGGATTCTTGTTGATAAACAACAAACATTATGT
    GAATTTTTCTATAATCCAGAAGGTGACACAAAATTAGAAAAGTATTTTTCTTACAAGGAT
    GGCAAACCAGAAGTACAAAAAATTATTGTTTACTATGCTAACAAACAATATTTCTTTAAT
    AATGAAACGGAATTAGGCGCATTTTTCATTAAACAACTATATCAACATGGGGATTTATTT
    TTCAGTGATCGTAATGTGTACACAGCACCTATTTTCAACTTAACGCCTGAGTCAATCCCA
    GTAGTAGCTGTGCTTCACAGTACACACATTAAAAATATTGATGCATTAGATTCATCGCCA
    TTTAAAAATGTATATAAAGCGATGTTTGAAAATTTATCTCGATATCGTGCAATTATCGTT
    TCTACTGAGCAGCAAAAATTAGATGTTGAAAAACGTATTAACCATACTATTCCAGTTGTT
    AATATTCCTGTCGGCTATAGTGAAACAATTGATACACCAGTCCAAACATTAGATCAGCGC
    AGTGTTAAATTAATTTCCGTTGCTCGTTATTCACCTGAAAAACAATTACATCAACAAATT
    GAATTAATTAAACGCTTAGTATCGTATGTCCCAAAAATAGAGCTTCATATGTATGGTTTT
    GGTTCTGAAAGTAAAAAATTAAATGAATTGATACAAAAATATGGATTGGAAAATCATGTT
    TACTTACGCGGCTTTTTAAGCAATCTAGACCAAGAATATAGCGATGCCTATTTGAGTTTA
    ATAACAAGTAACATGGAAGGTTTCTCTTTAGCGCTACTTGAATCTTTAGCACATGGTGTT
    CCAGTTATTAGTTATGATATTAAATATGGACCTAACGAACTGATTACTTCAGATTTCAAT
    GGTTATTTAATTACTAAAAATGATGAAGATGCCCTATTTGATAAAGTAAAATATGTGATT
    GATCATCCTGAAGTACAGCAACGACTGTCAAAAGGTAGTTTGGCAAAAGCACAACAATAT
    AGTAAAGCGTCGTTAATTAAACAATGGGACCAATTTGTTCGTCTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0550 [new locus tag: SAUSA300_RS02935 ]
  • symbol: SAUSA300_0550
  • description: glycosyl transferase, group 1 family protein
  • length: 496
  • theoretical pI: 6.88884
  • theoretical MW: 58417.9
  • GRAVY: -0.422177

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 130.3)
    and 12 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 78.1)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 64.3)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 61.3)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 27.6)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 27.4)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 25.3)
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosyltransferase GtfB (TIGR02919; EC 2.4.1.-; HMM-score: 24.7)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 24.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 22.9)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 20.4)
    colanic acid biosynthesis glycosyltransferase WcaC (TIGR04015; EC 2.4.-.-; HMM-score: 15.5)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 10.5)
  • TheSEED  :
    • Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 112.1)
    and 5 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 72.2)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 23.9)
    NTF2 (CL0051) Ring_hydroxyl_B; Ring hydroxylating beta subunit (PF00866; HMM-score: 17)
    no clan defined ETC_C1_NDUFA5; ETC complex I subunit conserved region (PF04716; HMM-score: 14.1)
    Dcc1; Sister chromatid cohesion protein Dcc1 (PF09724; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005522
    • TAT(Tat/SPI): 0.000307
    • LIPO(Sec/SPII): 0.001655
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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