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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0828 [new locus tag: NWMN_RS04675 ]
  • pan locus tag?: SAUPAN003060000
  • symbol: gudB
  • pan gene symbol?: gudB
  • synonym:
  • product: NAD-specific glutamate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0828 [new locus tag: NWMN_RS04675 ]
  • symbol: gudB
  • product: NAD-specific glutamate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 915759..917045
  • length: 1287
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    TTGAAAGCGAAATCATTAGGTAAAGAAGAGGCGAAAAGGATCATGACTGAGAACAATAAT
    TTAGTAACTTCTACTCAAGGAATTATTAAAGAAGCATTGCATAAATTGGGATTTGACGAA
    GGAATGTACGATTTAATTAAAGAACCTTTAAGAATGTTACAAGTGCGTATCCCTGTACGA
    ATGGATGATGGCACAGTAAAAACATTCACAGGTTACCGTGCGCAACATAATGATGCTGTT
    GGACCAACAAAAGGGGGCGTGCGTTTCCACCCAGATGTTGATGAAGAAGAAGTAAAAGCA
    TTATCAATGTGGATGACTTTGAAATGTGGCATTGTAAACTTACCATACGGTGGTGGTAAG
    GGTGGTATCGTTTGTGATCCACGTCAAATGAGCATTCATGAAGTTGAACGTTTATCACGC
    GGATATGTAAGAGCAATTTCACAATTCGTAGGTCCGAACAAAGATATTCCAGCACCAGAT
    GTATTTACAAACTCACAAATTATGGCTTGGATGATGGATGAATATAGTGCATTAGATAAA
    TTTAATTCACCAGGTTTCATCACAGGTAAACCAATTGTATTGGGTGGTTCTCATGGACGC
    GACAGATCAACTGCACTAGGTGTAGTTATTGCAATTGAACAAGCTGCAAAACGTCGTAAT
    ATGCAAATTGAAGGTGCCAAGGTTGTTATTCAAGGTTTCGGTAATGCCGGAAGTTTCTTA
    GCTAAATTCTTATATGATTTAGGTGCAAAAATTGTAGGTATCTCTGATGCTTACGGTGCA
    TTACACGATCCAAATGGCTTAGATATAGATTATTTATTAGACCGTCGTGATAGTTTTGGT
    ACGGTAACAAATTTATTTGAAGAAACAATCTCAAATAAAGAATTGTTTGAATTAGATTGT
    GACATTTTAGTACCAGCGGCTATTTCAAACCAAATTACAGAAGACAATGCACATGATATT
    AAAGCTAGTATCGTTGTTGAAGCTGCTAATGGACCTACAACACCAGAAGCAACACGTATT
    TTAACTGAACGTGGTATATTATTAGTTCCAGACGTATTAGCAAGTGCTGGTGGTGTAACG
    GTTTCTTACTTCGAATGGGTACAAAATAATCAAGGTTATTATTGGTCTGAAGAAGAAGTT
    AATGAAAAGCTACGTGAAAAATTAGAAGCGGCATTTGATACGATTTACGAATTGTCTCAA
    AACCGAAAAATAGATATGAGACTTGCAGCATATATCATAGGTATTAAACGTACAGCAGAA
    GCAGCTAGATATCGTGGTTGGGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0828 [new locus tag: NWMN_RS04675 ]
  • symbol: GudB
  • description: NAD-specific glutamate dehydrogenase
  • length: 428
  • theoretical pI: 5.36927
  • theoretical MW: 47330.6
  • GRAVY: -0.23785

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationdipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 11.8)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamate dehydrogenases NAD-specific glutamate dehydrogenase (EC 1.4.1.2) 
    Glutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis NAD-specific glutamate dehydrogenase (EC 1.4.1.2) 
  • PFAM:
    NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 262.1)
    AA_dh_N (CL0603) ELFV_dehydrog_N; Glu/Leu/Phe/Val dehydrogenase, dimerisation domain (PF02812; HMM-score: 188.6)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.7)
    no clan definedCobS_N; Cobaltochelatase CobS subunit N terminal (PF12556; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_1156(sucD)succinyl-CoA synthetase subunit alpha  [1] (data from MRSA252)
    NWMN_1763ABC transporter ATP-binding protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.13
    • Ymax_pos: 11
    • Cmax: 0.108
    • Cmax_pos: 32
    • Smax: 0.227
    • Smax_pos: 4
    • Smean: 0.166
    • D: 0.144
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKAKSLGKEEAKRIMTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEYSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGIKRTAEAARYRGWA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]