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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0941 [new locus tag: NWMN_RS05270 ]
  • pan locus tag?: SAUPAN003286000
  • symbol: purH
  • pan gene symbol?: purH
  • synonym:
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0941 [new locus tag: NWMN_RS05270 ]
  • symbol: purH
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1044582..1046060
  • length: 1479
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCG
    TTAACGCAATTAAATTATGAATTATATTCAACAGGTGGTACTAAACGTATATTAGATGAA
    GCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATTTTCCAGAAATAATGGATGGC
    CGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAACCG
    CAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAAT
    TTATATCCATTCCAACAAACTGTTGCAAACCCTGATGTGACGATGGACGAAGCAATTGAA
    AATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAGCTAAAAACTATAAACATGTA
    ACAACAATTGTACATCCGGCAGATTATCAAGAAGTATTGACGCTATTAAGAAACGATTCG
    TTAGATGAGTCATATAGACAATCATTAATGATTAAAGTTTTTGAGCATACTGCAGAATAT
    GATGAAGCGATTGTACGTTTCTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAAT
    CCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTAAGCACACGATTGCAGGCGCT
    AAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACACTA
    GCTTTAGTTAAAAAGTTTGATACACCTGCTACAGTTGCGGTTAAACACATGAATCCATGT
    GGTGTTGGTATCGGTGACACGATAGAACAAGCATTTCAACATGCGTATGAAGCGGATAGT
    CAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTGTAACACCTGAGTTAGCAGAG
    CAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCATTA
    GATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGACAGT
    AACGAAGAAGAGTTTGTTTCAGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTAT
    GTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTAGCACCTACAGATGAACAATGG
    GAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATTTTA
    AGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCT
    AAAATTGCGTTAGAGAGAGCTATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGA
    TTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAACATGGTATAAAGGCAATTATC
    CAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGTATT
    GCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0941 [new locus tag: NWMN_RS05270 ]
  • symbol: PurH
  • description: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • length: 492
  • theoretical pI: 5.80622
  • theoretical MW: 54304.5
  • GRAVY: -0.207317

Function[edit source | edit]

  • reaction:
    EC 3.5.4.10?  ExPASy
    IMP cyclohydrolaseIMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
    EC 2.1.2.3?  ExPASy
    Phosphoribosylaminoimidazolecarboxamide formyltransferase10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (TIGR00355; EC 2.1.2.3,3.5.4.10; HMM-score: 642.3)
  • TheSEED:  
    De Novo Purine Biosynthesis IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) 
  • PFAM:
    CDA (CL0109) AICARFT_IMPCHas; AICARFT/IMPCHase bienzyme (PF01808; HMM-score: 359.6)
    no clan definedMGS; MGS-like domain (PF02142; HMM-score: 106.3)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 16.8)
    EFTPs (CL0575) NSP2_assoc; Non-essential region of nsp2 of arterivirus polyprotein (PF14758; HMM-score: 15)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.247
    • Ymax_pos: 21
    • Cmax: 0.193
    • Cmax_pos: 21
    • Smax: 0.402
    • Smax_pos: 7
    • Smean: 0.319
    • D: 0.275
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTLLRNDSLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]