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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1239 [new locus tag: NWMN_RS06995 ]
  • pan locus tag?: SAUPAN003698000
  • symbol: thrA
  • pan gene symbol?: thrD
  • synonym:
  • product: aspartate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1239 [new locus tag: NWMN_RS06995 ]
  • symbol: thrA
  • product: aspartate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1361802..1363196
  • length: 1395
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    TTGGCATGCACTATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAA
    ATTAAAAAGGTTTTAAATATTGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCT
    GCTCCAGGTAAAAGACATGATAATGATATTAAAACAACTGATTTGTTAATCAGATTATAT
    GAAAAGGTCATTAATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTAT
    GATGATATTGTAAAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACT
    TTGGAACATTATATTAATCAATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTT
    TCTTGTGGTGAAGATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTCAAGGTATA
    CCAACAAAATATATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAA
    GCACAAATATTAGATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAA
    CTAATTATCCCAGGATTTTTTGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGT
    GGCGGATCAGATATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAATTTATGAA
    AATTTCACCGATGTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAA
    TTAATCGAAGAAATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTT
    TTCCATGATGAAGCCTTACAACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAAT
    ACGAACCGCCCTCAAGATAAGGGTACATTTATAGTGCATGATAGAGAAATAAATGCGAAA
    AATGTGATTAGCGGTATAAGTTGTGATAAAGATTTTACCGTTATTAATATCAAAAAGTAT
    TTAATGAATAGACAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAAT
    ATTTCATTTGATCATATGCCTTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAA
    CAAATTCAAAATAAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTGATGTTGAT
    GAGCTAAGTATTGAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAA
    GTCATCGGTACTGCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAA
    ATGATTAACCAAGGTGCTTCGGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCT
    GAAAAAGCTGTATTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAAAAAACTTG
    TGTGATAAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1395

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1239 [new locus tag: NWMN_RS06995 ]
  • symbol: ThrA
  • description: aspartate kinase
  • length: 464
  • theoretical pI: 6.38574
  • theoretical MW: 52409.8
  • GRAVY: -0.266595

Function[edit source | edit]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinaseATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyaspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 285.4)
    MetabolismAmino acid biosynthesisAspartate familyaspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 255.3)
    MetabolismAmino acid biosynthesisAspartate familyputative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 91.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsputative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 20.5)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway Aspartokinase (EC 2.7.2.4) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Aspartokinase (EC 2.7.2.4) 
  • PFAM:
    no clan definedAA_kinase; Amino acid kinase family (PF00696; HMM-score: 142.7)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 35.9)
    ACT_7; ACT domain (PF13840; HMM-score: 31.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.302
    • Ymax_pos: 19
    • Cmax: 0.206
    • Cmax_pos: 19
    • Smax: 0.674
    • Smax_pos: 3
    • Smean: 0.497
    • D: 0.378
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MACTMKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • thrA no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]