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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1287 [new locus tag: NWMN_RS07250 ]
  • pan locus tag?: SAUPAN003770000
  • symbol: femB
  • pan gene symbol?: femB
  • synonym:
  • product: methicillin resistance expression factor FemB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1287 [new locus tag: NWMN_RS07250 ]
  • symbol: femB
  • product: methicillin resistance expression factor FemB
  • replicon: chromosome
  • strand: +
  • coordinates: 1418312..1419571
  • length: 1260
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAATTTACAGAGTTAACTGTTACCGAATTTGACAACTTTGTACAAAATCCATCATTG
    GAAAGTCATTATTTCCAAGTAAAAGAAAATATAGTTACCCGTGAGAATGATGGCTTTGAA
    GTAGTTTTATTAGGTATTAAAGACGACAATAACAAAGTAATTGCAGCAAGCCTTTTCTCT
    AAAATTCCTACTATGGGAAGTTATGTTTACTATTCGAATCGTGGTCCAGTAATGGATTTT
    TCAGATTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACAT
    CAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCATCTATATGATAAAGATATC
    GTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGT
    TACGAGCATCATGGCTTTACAACTGAGTATGATACATCGAGCCAAGTACGATGGATGGGC
    GTATTAAACCTTGAAGGTAAAACACCCGAAACATTGAAAAAGACATTTGATAGTCAACGT
    AAACGTAATATTAATAAAGCGATAAACTATGGTGTTAAAGTCAGATTCCTTGAACGTGAT
    GAGTTCAATCTTTTCTTAGATTTATATCGTGAAACTGAAGAGCGTGCTGGATTTGTATCA
    AAAACAGATGATTATTTTTATAACTTTATTGACACATATGGAGATAAAGTATTAGTACCA
    TTAGCATATATTGACCTTGATGAATATGTGTTAAAGTTGCAACAGGAATTGAATGACAAA
    GAAAATCGTCGTGATCAAATGATGGCGAAAGAAAACAAATCAGATAAGCAAATGAAGAAA
    ATTGCAGAATTAGATAAGCAAATTGATCATGATCAGCATGAATTATTGAATGCAAGTGAA
    TTGAGCAAAACGGACGGCCCAATTCTAAACCTTGCTTCTGGCGTTTATTTTGCAAATGCA
    TATGAAGTGAATTATTTCTCTGGTGGTTCATCAGAAAAATATAATCAATTTATGGGACCA
    TACATGATGCATTGGTTTATGATTAACTATTGCTTCGATAATGGCTATGATCGTTATAAT
    TTCTATGGTTTATCAGGTGATTTTACGGAAAACAGTGAAGATTATGGCGTATACCGCTTT
    AAACGTGGATTTAATGTACAAATCGAAGAATTAATAGGGGATTTCTATAAACCAATTCAT
    AAAGTGAAATATTGGTTGTTCACAACATTGGATAAATTACGTAAAAAATTAAAGAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1287 [new locus tag: NWMN_RS07250 ]
  • symbol: FemB
  • description: methicillin resistance expression factor FemB
  • length: 419
  • theoretical pI: 5.37676
  • theoretical MW: 49675.6
  • GRAVY: -0.649881

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage major capsid protein, HK97 family (TIGR01554; HMM-score: 12.1)
  • TheSEED:  
    tRNA-dependent amino acid transfers FemB, factor involved in methicillin resistance 
    tRNA-dependent amino acid transfers tRNA-dependent lipid II-GlyGlyGly glycyltransferase (EC 2.3.2.18) 
    tRNA-dependent amino acid transfers tRNA-dependent lipid II-GlyGlyGlyGly glycyltransferase (EC 2.3.2.18) 
  • PFAM:
    Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 471.9)
    Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 18.7)
    no clan definedDUF1497; Protein of unknown function (DUF1497) (PF07384; HMM-score: 14.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.127
    • Ymax_pos: 28
    • Cmax: 0.163
    • Cmax_pos: 56
    • Smax: 0.191
    • Smax_pos: 19
    • Smean: 0.109
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMGVLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIHKVKYWLFTTLDKLRKKLKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]