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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1452 [new locus tag: NWMN_RS08195 ]
  • pan locus tag?: SAUPAN004129000
  • symbol: NWMN_1452
  • pan gene symbol?:
  • synonym:
  • product: peptidase, rhomboid family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1452 [new locus tag: NWMN_RS08195 ]
  • symbol: NWMN_1452
  • product: peptidase, rhomboid family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1619257..1620720
  • length: 1464
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAACATAGACAAACAATTTTGGAAAACAATATATTATTGGATTAGGTACTTGAATTTT
    GATATTGTCAGTAGGGAAAAAGATGACCAAGAAATTTGGTTAGCCCATAAAAGAAAAAAA
    CAAGTAGTCATTTTTAAACAACATATCAAGTCTACCCAAGAAATACGTTTTGACAAAGCG
    AAAGTGCTTGAACACAAAGATGAAATAGCAAATTTTATTTCTTTCGAACCACAAAGTTTT
    GAATTTTATTATTTTACAGAATCGGAATTTTCAGAAGAACAATTAAATGAAGTTTCGCCA
    ATTAGAATTAAATTCAATGTTATAAGACACACAAAAGATTTGATAAAGCATATGCCGAAT
    ATATTTTTGGCTAGACTTATTTCAGAAGATAATGATAAAAAGACATATATGTTCTATAAA
    CGCAAAGTATTAACCGATAACTTTTTAGATAAATATATGCAGAAATTTTCACCGGCAACA
    TACACAATAATATTTGTAAATGTCTTAATATGGTTATGTATGATTTTATATTTAAATAAT
    TTTTCGGATGTAAAATTATTAGATGTTGGCGGGTTAGTGCATTTTAATGTCGTACATGGT
    GAATGGTATCGAATTGTTACATCGATGTTTTTACATTTTAGTTTTGAACATATACTTATG
    AATATGCTTTCATTATTTATTTTTGGTAAAATAGTCGAAGCAATTATTGGTTCATGGCGG
    ATGTTAACTGTATACTTTATTGCAGGGTTGTTTGGAAACTTTGTATCACTATCATTTAAT
    ACGACTACAATTTCAGTTGGGGCTAGTGGTGCTATATTTGGTCTGATTGGATCAATTTTT
    GCGATGATGTATGTTTCAAAAACATTTAACAAAAAAATGTTAGGACAGTTATTAATTGCA
    TTAGTGATATTAGTTGGTGTTTCTCTGTTTATGTCAAATATAAATATTGTGGCGCATATT
    GGAGGATTCATTGGTGGTTTATTAATAACTTTAATTGGCTATTACTATAAAGTGAATCGT
    AATATTTTTTGGATTTTACTAATTGGTATGCTTGTTATATTTATTGCACTTCAAATTAGA
    ATTTTTACAATTAAAGAAGATAATATTTATAATAAATTGATCAAAGATGATATGACTAGT
    GGTAATTATGATAATGCTCAAAATATTGTGAAGCAGACAATAAATAAAAATTATGCCGAT
    GATCAAACATATTATTTAAGTGGTATGATTATGGCTACTATCAATTCTAAGTCCGAAGGT
    ATGACAGAATGGGAGCGAGGACTTAGAATGTTTCCTAAATCAGGTTTATTAAATTTTGAG
    TTAGCGATAGCAAATCGTTCATTAAATGATGATGAAAAAGCATTAAAATATGTGCGTAAA
    GCATTAAATGCAGACCCTAAAAATACAGATTATATTAACTTAGAAAAAGAGTTGACTAAA
    TCAAATGAGTCGAAAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1464

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1452 [new locus tag: NWMN_RS08195 ]
  • symbol: NWMN_1452
  • description: peptidase, rhomboid family protein
  • length: 487
  • theoretical pI: 9.54533
  • theoretical MW: 56889.1
  • GRAVY: 0.0568788

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    rhomboid family protease GlpG (TIGR04239; EC 3.4.21.105; HMM-score: 54.7)
    rhombosortase (TIGR03902; EC 3.4.21.-; HMM-score: 42.9)
    putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 20.7)
    type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 17.2)
  • TheSEED:  
    FIG056164: rhomboid family serine protease 
  • PFAM:
    Rhomboid-like (CL0207) Rhomboid; Rhomboid family (PF01694; HMM-score: 119.3)
    TPR (CL0020) Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 20.6)
    TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 20.3)
    TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 17)
    TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 15.7)
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 14.4)
    TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 13.7)
    no clan definedSensor_TM1; Sensor N-terminal transmembrane domain (PF13755; HMM-score: 10.2)
    O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 5.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.095
    • Ymax_pos: 70
    • Cmax: 0.107
    • Cmax_pos: 29
    • Smax: 0.101
    • Smax_pos: 58
    • Smean: 0.067
    • D: 0.084
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVIFKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIRIKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPATYTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLIGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGYYYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]