From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1659 [new locus tag: NWMN_RS09315 ]
  • pan locus tag?: SAUPAN004455000
  • symbol: ribH
  • pan gene symbol?: ribH
  • synonym:
  • product: 6,7-dimethyl-8-ribityllumazine synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1659 [new locus tag: NWMN_RS09315 ]
  • symbol: ribH
  • product: 6,7-dimethyl-8-ribityllumazine synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1855450..1855914
  • length: 465
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATTTTGAAGGTAAATTAATTGGAAAAGATTTGAAAGTTGCAATCGTAGTTAGTCGA
    TTTAATGATTTTATCACTGGAAGATTACTTGAAGGTGCAAAAGATACTTTGATTCGACAT
    GATGTTAATGAAGACAATATTGATGTAGCATTTGTGCCAGGTGCGTTTGAAATTCCTTTA
    GTAGCTAAAAAATTAGCCTCATCAGGAAATTATGATGCAGTAATTACATTAGGATGCGTA
    ATTCGCGGTGCTACGTCTCATTATGATTATGTTTGTAATGAAGTCGCGAAAGGTGTTTCT
    AAAGTGAATGATCAAACTAATGTACCAGTCATATTTGGTATTTTAACGACTGAAAGTATT
    GAACAAGCTGTGGAAAGAGCAGGTACGAAAGCTGGCAATAAAGGTGCTGAAGCAGCAGTA
    AGTGCAATTGAAATGGCTAATTTATTAAAATCTATAAAAGCATAG
    60
    120
    180
    240
    300
    360
    420
    465

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1659 [new locus tag: NWMN_RS09315 ]
  • symbol: RibH
  • description: 6,7-dimethyl-8-ribityllumazine synthase
  • length: 154
  • theoretical pI: 5.78192
  • theoretical MW: 16395.7
  • GRAVY: 0.127273

Function[edit source | edit]

  • reaction:
    EC 2.5.1.78?  ExPASy
    6,7-dimethyl-8-ribityllumazine synthase1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersRiboflavin, FMN, and FAD6,7-dimethyl-8-ribityllumazine synthase (TIGR00114; EC 2.5.1.78; HMM-score: 198)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersRiboflavin, FMN, and FADriboflavin synthase (TIGR01506; EC 2.5.1.9; HMM-score: 21.6)
    MetabolismAmino acid biosynthesisAromatic amino acid family3-dehydroquinate dehydratase, type II (TIGR01088; EC 4.2.1.10; HMM-score: 12.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsRiboflavin, FMN, FADRiboflavin, FMN and FAD metabolism Extended 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78) 
  • PFAM:
    no clan definedDMRL_synthase; 6,7-dimethyl-8-ribityllumazine synthase (PF00885; HMM-score: 202.7)
    TIM_barrel (CL0036) Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.133
    • Ymax_pos: 34
    • Cmax: 0.117
    • Cmax_pos: 34
    • Smax: 0.307
    • Smax_pos: 29
    • Smean: 0.133
    • D: 0.133
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNEDNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKVNDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]