From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1660 [new locus tag: NWMN_RS09320 ]
  • pan locus tag?: SAUPAN004456000
  • symbol: ribA
  • pan gene symbol?: ribA
  • synonym:
  • product: riboflavin biosynthesis protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1660 [new locus tag: NWMN_RS09320 ]
  • symbol: ribA
  • product: riboflavin biosynthesis protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1855927..1857108
  • length: 1182
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAATTCGATAATATTGACAGTGCTTTAATGGCTTTAAAAAATGGAGAACCAATTATT
    GTAGTAGATGATGAGAATCGTGAAAATGAAGGTGATTTAGTAGCGGTTACTGAATGGATG
    AACGATAATACCATTAATTTTATGGCGAAAGAAGCAAGGGGATTAATATGCGCACCAGTG
    TCTAAAGATATTGCACAACGTTTGGATTTGGTACAAATGGTTGATGATAACTCCGACATC
    TTTGGTACGCAATTTACAGTGAGTATTGATCATGTAGATACAACAACAGGAATTAGTGCT
    TATGAACGTACATTGACTGCCAAAAAGCTCATTGATCCTAGTAGTGAAGCTAAAGATTTT
    AATCGTCCTGGTCATTTATTTCCATTAGTAGCACAAGATAAAGGCGTATTAGCTAGAAAT
    GGACACACAGAAGCGGCTGTTGATTTAGCTAAACTTACTGGTGCCAAGCCCGCTGGTGTC
    ATTTGTGAGATTATGAATGATGACGGCACGATGGCGAAAGGACAAGATTTACAAAAGTTT
    AAAGAAAAACATCAATTAAAGATGATTACGATTGATGATTTAATTGAATATCGTAAAAAA
    TTAGAACCAGAAATTGAATTTAAGGCAAAAGTGAAAATGCCTACAGATTTCGGAACATTT
    GATATGTATGGTTTTAAAGCGACATACACAGATGAAGAGATAGTTGTACTGACAAAAGGT
    GCAATTCGACAACATGAAAATGTACGCTTACATTCTGCGTGCCTTACAGGCGATATTTTC
    CATAGTCAACGTTGTGATTGTGGTGCTCAACTTGAATCGTCTATGAAGTATATCAATGAA
    CATGGTGGCATGATTATTTATCTACCTCAAGAAGGTCGTGGCATAGGATTGTTAAACAAA
    TTACGCGCATATGAATTAATTGAGCAAGGATATGATACAGTAACTGCAAATTTAGCATTA
    GGTTTTGATGAAGATTTGCGAGATTATCATATTGCTGCACAGATTTTAAAATATTTTAAC
    ATCGAACATATCAATTTATTAAGTAATAATCCAAGTAAATTTGAGGGATTAAAACAATAT
    GGCATTGATATTGCAGAAAGAATTGAAGTTATCGTACCAGAAACGGTACATAATCATGAT
    TATATGGAAACGAAAAAAATAAAAATGGGTCATTTAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1660 [new locus tag: NWMN_RS09320 ]
  • symbol: RibA
  • description: riboflavin biosynthesis protein
  • length: 393
  • theoretical pI: 4.88903
  • theoretical MW: 44188.1
  • GRAVY: -0.326718

Function[edit source | edit]

  • reaction:
    D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate?
    GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate?
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersRiboflavin, FMN, and FAD3,4-dihydroxy-2-butanone-4-phosphate synthase (TIGR00506; EC 4.1.99.12; HMM-score: 247.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersRiboflavin, FMN, and FADGTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 227)
  • TheSEED:  
    Folate Biosynthesis 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) 
    Molybdenum cofactor biosynthesis 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) 
    riboflavin to FAD 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) 
  • PFAM:
    no clan definedDHBP_synthase; 3,4-dihydroxy-2-butanone 4-phosphate synthase (PF00926; HMM-score: 268)
    GTP_cyclohydro2; GTP cyclohydrolase II (PF00925; HMM-score: 209.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+, Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 28
    • Cmax: 0.12
    • Cmax_pos: 50
    • Smax: 0.161
    • Smax_pos: 23
    • Smean: 0.097
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHLI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]