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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1748 [new locus tag: NWMN_RS09850 ]
  • pan locus tag?: SAUPAN004792000
  • symbol: NWMN_1748
  • pan gene symbol?: queG
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1748 [new locus tag: NWMN_RS09850 ]
  • symbol: NWMN_1748
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1948916..1950043
  • length: 1128
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGGATACAAAGCAATTAAAGCAAGATATCATTGACTATGCATATACAATTGGTATCGAC
    AGTATTGGATTTACTACTGCCGATCCCTTTGATGAATTGAAGCAAAAGCTAGAAGCATAT
    CATGCAAATGGTTATGCCTCAGGATTTGAAGAATCTGATATTGCTTTACGAACGGAGCCT
    AAATTATCCTTACCAACAGCAAGGTCAATCATAGCAATTGCAGTTGGTTATCCTAACAAA
    CTGAAAGGTGCACCTAAGAGTGTTAGAGGTGATCGCAGAGGCTTATTTGCTAGAGCATCG
    TGGGGTCAAGATTATCATACAATTATGCGTAAACGATTAGACATGTTAGCTGCATTTATT
    GAATCTAAAGTTCCAGATGTTGAAATCAAATCTATGGTAGATACGGGTGTATTATCAGAT
    AGGGCAGTAGCAGAACGTGCAGGTTTAGGATTTGTTGGTCGTAATGGCTTTGTCATCAAT
    CCTAAACTAGGAACATGGACATACCTCGGTGAAATGTTAGTCAGTATACCTTTTGAACCT
    GATGATCCATTATTAGATAGCTGTGGTGATTGTACAATTTGTGTTGATCGTTGTCCAACA
    AGTGCATTAGTTGGAAATGGCCAATTAAATAGTCAGAAATGCATTAGTTTTTTAACGCAA
    ACAAAAGGCTATATGCCTGACCAATATCGTTATAAAATTGGGAATAGATTATATGGTTGC
    GATACGTGTCAACAAGTTTGTCCGAAAAATAGAGGTATTAATACCGAACAAGATGACATC
    ATTTTGGAACCAGAAATTTTAAAGCCAAGATTAGTACCTTTACTACGTATGTCTAATAAA
    GAATTCAAACAAACATATGGTCACCTTGCAGGTGCTTGGCGTGGTAAAAAGCCGATACAA
    CGAAATGCTATTTTAGCATTGGCGCATTTTAATGAAGTGGATGCAATTCCAGAATTGAAA
    AAAGTTGCAACAACAGATGAAAGACCGTTGATTCGAGCAACTGCTTATTGGGCCATTGGT
    CAAATTCTTGGGGAAGAAGCAAGAGATTTTATCAATGCTAATTATGATCAAGAGGATGCA
    GAAGTTCAGAATGAAATGATAAAAGGATTAGACACAAGGAGAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1128

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1748 [new locus tag: NWMN_RS09850 ]
  • symbol: NWMN_1748
  • description: hypothetical protein
  • length: 375
  • theoretical pI: 6.17984
  • theoretical MW: 41935.6
  • GRAVY: -0.375467

Function[edit source | edit]

  • reaction:
    EC 1.17.99.6?  ExPASy
    Epoxyqueuosine reductaseQueuosine34 in tRNA + acceptor + H2O = epoxyqueuosine34 in tRNA + reduced acceptor
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationepoxyqueuosine reductase (TIGR00276; EC 1.-.-.-; HMM-score: 401.3)
    reductive dehalogenase (TIGR02486; EC 1.97.1.-; HMM-score: 45.2)
    2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family (TIGR02179; HMM-score: 19.8)
    MetabolismEnergy metabolismElectron transportelectron transport complex, RnfABCDGE type, B subunit (TIGR01944; HMM-score: 15.5)
    MetabolismEnergy metabolismElectron transportNADH-quinone oxidoreductase, chain I (TIGR01971; HMM-score: 11.7)
    MetabolismEnergy metabolismChemoautotrophyCO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00314; EC 1.2.99.2; HMM-score: 10.7)
    Unknown functionGeneralarchaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 10.5)
    pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 7.7)
    Genetic information processingProtein fateProtein modification and repairglycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 6.9)
    MetabolismCentral intermediary metabolismNitrogen fixationferredoxin III, nif-specific (TIGR02936; HMM-score: 5.5)
    methylamine methyltransferase corrinoid protein reductive activase (TIGR04270; HMM-score: 3.7)
    MetabolismEnergy metabolismMethanogenesisputative methanogenesis marker 16 metalloprotein (TIGR03287; HMM-score: 2.6)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationQueuosine-Archaeosine Biosynthesis tRNA epoxyqueuosine(34) reductase (EC 1.17.99.6) 
    Soluble cytochromes and functionally related electron carriers tRNA epoxyqueuosine(34) reductase (EC 1.17.99.6) 
  • PFAM:
    no clan definedDUF1730; Domain of unknown function (DUF1730) (PF08331; HMM-score: 62.9)
    4Fe-4S (CL0344) Fer4_16; 4Fe-4S double cluster binding domain (PF13484; HMM-score: 54.7)
    TPR (CL0020) HEAT_2; HEAT repeats (PF13646; HMM-score: 32)
    4Fe-4S (CL0344) Fer4_9; 4Fe-4S dicluster domain (PF13187; HMM-score: 26.1)
    Fer4_7; 4Fe-4S dicluster domain (PF12838; HMM-score: 24.8)
    Fer4_10; 4Fe-4S dicluster domain (PF13237; HMM-score: 24.1)
    Fer4; 4Fe-4S binding domain (PF00037; HMM-score: 18.6)
    Fer4_21; 4Fe-4S dicluster domain (PF14697; HMM-score: 17.9)
    Fer4_6; 4Fe-4S binding domain (PF12837; HMM-score: 14.8)
    TPR (CL0020) HEAT_PBS; PBS lyase HEAT-like repeat (PF03130; HMM-score: 13.1)
    HEAT; HEAT repeat (PF02985; HMM-score: 12.9)
    FMN-dep-NRtase (CL0529) Dehalogenase; Reductive dehalogenase subunit (PF13486; HMM-score: 12.7)
    4Fe-4S (CL0344) Fer4_2; 4Fe-4S binding domain (PF12797; HMM-score: 12.5)
    Fer4_8; 4Fe-4S dicluster domain (PF13183; HMM-score: 12.3)
    Fer4_3; 4Fe-4S binding domain (PF12798; HMM-score: 11.6)
    Fer4_17; 4Fe-4S dicluster domain (PF13534; HMM-score: 11.2)
    Fer4_13; 4Fe-4S single cluster domain of Ferredoxin I (PF13370; HMM-score: 10.1)
    Fer4_18; 4Fe-4S dicluster domain (PF13746; HMM-score: 7.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 42
    • Cmax: 0.11
    • Cmax_pos: 30
    • Smax: 0.175
    • Smax_pos: 37
    • Smean: 0.085
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANGYASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWGQDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVINPKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFLTQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLRMSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRATAYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]