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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01989
  • pan locus tag?: SAUPAN004792000
  • symbol: SAOUHSC_01989
  • pan gene symbol?: queG
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01989
  • symbol: SAOUHSC_01989
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1892067..1893209
  • length: 1143
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGGGCATACGATTGGATACAAAGCAATTAAAGCAAGATATCATTGACTATGCATAT
    ACAATTGGTATCGACAGTATTGGATTTACTACTGCCGATCCCTTTGATGAATTGAAGCAA
    AAGCTAGAAGCATATCATGCAAATGGTTATGCCTCAGGATTTGAAGAATCTGATATTGCT
    TTACGAACGGAGCCTAAATTATCCTTACCAACAGCAAGGTCAATCATAGCAATTGCAGTT
    GGTTATCCTAACAAACTGAAAGGTGCACCTAAGAGTGTTAGAGGTGATCGCAGAGGCTTA
    TTTGCTAGAGCATCGTGGGGTCAAGATTATCATACAATTATGCGTAAACGATTAGACATG
    TTAGCTGCATTTATTGAATCTAAAGTTCCAGATGTTGAAATCAAATCTATGGTAGATACG
    GGTGTATTATCAGATAGGGCAGTAGCAGAACGTGCAGGTTTAGGATTTGTTGGTCGTAAT
    GGCTTTGTCATCAATCCTAAACTAGGAACATGGACATACCTCGGTGAAATGTTAGTCAGT
    ATACCTTTTGAACCTGATGATCCATTATTAGATAGCTGTGGTGATTGTACAATTTGTGTT
    GATCGTTGTCCAACAAGTGCATTAGTTGGAAATGGCCAATTAAATAGTCAGAAATGCATT
    AGTTTTTTAACGCAAACAAAAGGCTATATGCCTGACCAATATCGTTATAAAATTGGGAAT
    AGATTATATGGTTGCGATACGTGTCAACAAGTTTGTCCGAAAAATAGAGGTATTAATACC
    GAACAAGATGACATCATTTTGGAACCAGAAATTTTAAAGCCAAGATTAGTACCTTTACTA
    CGTATGTCTAATAAAGAATTCAAACAAACATATGGTCACCTTGCAGGTGCTTGGCGTGGT
    AAAAAGCCGATACAACGAAATGCTATTTTAGCATTGGCGCATTTTAATGAAGTGGATGCA
    ATTCCAGAATTGAAAAAAGTTGCAACAACAGATGAAAGACCGTTGATTCGAGCAACTGCT
    TATTGGGCCATTGGTCAAATTCTTGGGGAAGAAGCAAGAGATTTTATCAATGCTAATTAT
    GATCAAGAGGATGCAGAAGTTCAGAATGAAATGATAAAAGGATTAGACACAAGGAGAGAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01989
  • symbol: SAOUHSC_01989
  • description: hypothetical protein
  • length: 380
  • theoretical pI: 6.52454
  • theoretical MW: 42432.2
  • GRAVY: -0.362632

Function[edit source | edit]

  • reaction:
    EC 1.17.99.6?  ExPASy
    Epoxyqueuosine reductaseQueuosine34 in tRNA + acceptor + H2O = epoxyqueuosine34 in tRNA + reduced acceptor
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationepoxyqueuosine reductase (TIGR00276; EC 1.-.-.-; HMM-score: 401.2)
    reductive dehalogenase (TIGR02486; EC 1.97.1.-; HMM-score: 45.1)
    2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family (TIGR02179; HMM-score: 19.8)
    MetabolismEnergy metabolismElectron transportelectron transport complex, RnfABCDGE type, B subunit (TIGR01944; HMM-score: 15.5)
    MetabolismEnergy metabolismElectron transportNADH-quinone oxidoreductase, chain I (TIGR01971; HMM-score: 11.6)
    MetabolismEnergy metabolismChemoautotrophyCO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00314; EC 1.2.99.2; HMM-score: 10.7)
    Unknown functionGeneralarchaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 10.4)
    pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 7.7)
    Genetic information processingProtein fateProtein modification and repairglycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 6.9)
    MetabolismCentral intermediary metabolismNitrogen fixationferredoxin III, nif-specific (TIGR02936; HMM-score: 5.3)
    methylamine methyltransferase corrinoid protein reductive activase (TIGR04270; HMM-score: 3.7)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationQueuosine-Archaeosine Biosynthesis tRNA epoxyqueuosine(34) reductase (EC 1.17.99.6) 
    Soluble cytochromes and functionally related electron carriers tRNA epoxyqueuosine(34) reductase (EC 1.17.99.6) 
  • PFAM:
    no clan definedDUF1730; Domain of unknown function (DUF1730) (PF08331; HMM-score: 62.9)
    4Fe-4S (CL0344) Fer4_16; 4Fe-4S double cluster binding domain (PF13484; HMM-score: 54.7)
    TPR (CL0020) HEAT_2; HEAT repeats (PF13646; HMM-score: 32)
    4Fe-4S (CL0344) Fer4_9; 4Fe-4S dicluster domain (PF13187; HMM-score: 26.1)
    Fer4_7; 4Fe-4S dicluster domain (PF12838; HMM-score: 24.7)
    Fer4_10; 4Fe-4S dicluster domain (PF13237; HMM-score: 24.1)
    Fer4; 4Fe-4S binding domain (PF00037; HMM-score: 18.5)
    Fer4_21; 4Fe-4S dicluster domain (PF14697; HMM-score: 17.8)
    Fer4_6; 4Fe-4S binding domain (PF12837; HMM-score: 14.8)
    TPR (CL0020) HEAT_PBS; PBS lyase HEAT-like repeat (PF03130; HMM-score: 13.1)
    HEAT; HEAT repeat (PF02985; HMM-score: 12.8)
    FMN-dep-NRtase (CL0529) Dehalogenase; Reductive dehalogenase subunit (PF13486; HMM-score: 12.6)
    4Fe-4S (CL0344) Fer4_2; 4Fe-4S binding domain (PF12797; HMM-score: 12.4)
    Fer4_8; 4Fe-4S dicluster domain (PF13183; HMM-score: 12.3)
    Fer4_3; 4Fe-4S binding domain (PF12798; HMM-score: 11.6)
    Fer4_17; 4Fe-4S dicluster domain (PF13534; HMM-score: 11.3)
    Fer4_13; 4Fe-4S single cluster domain of Ferredoxin I (PF13370; HMM-score: 10)
    Fer4_18; 4Fe-4S dicluster domain (PF13746; HMM-score: 7.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 47
    • Cmax: 0.109
    • Cmax_pos: 38
    • Smax: 0.161
    • Smax_pos: 42
    • Smean: 0.079
    • D: 0.097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGGIRLDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANGYASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWGQDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVINPKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFLTQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLRMSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRATAYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]