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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01991
  • pan locus tag?: SAUPAN004794000
  • symbol: SAOUHSC_01991
  • pan gene symbol?:
  • synonym:
  • product: ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01991
  • symbol: SAOUHSC_01991
  • product: ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1894060..1895517
  • length: 1458
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGTGTTTAATTAGGTTTATATTAGTATTAGGGTTGTTAATTAGCAGTGCTATGGTG
    TATATCAATCCCACAGCACATGCTGAACAAGATCAAACATGGGAGAAGATTAAAGAACGC
    GGTGAACTTAGAGTGGGTCTTTCTGCAGATTATGCACCAATGGAATTTGAGCATACAGTT
    AATGGTAAGACTGAGTATGCAGGTGTAGATATTGATTTAGCTAAAAAAATTGCGAAAGAT
    AATAATTTAAAATTAAAAATCGTCAATATGTCATTTGATAGTTTGTTAGGAGCTCTTAAA
    ACTGGAAAAATTGATATTATTATTTCCGGAATGACTTCAACGCCTGAACGTAAGAAGCAA
    GTTGATTTTTCAGATTCATATATGATGACTAAAAATATCATGCTTGTAAAGAAAGATAAA
    GTTAATGAATATAAAGATATCAAAGACTTTAATAATAAAAAAGTAGGGGCACAAAAGGGA
    ACTGAACAAGAAAAAATCGCTCAAACCGAAATTGAAAATGCATCTATTACTTCATTAAGC
    CGTTTACCAGACGTTATATTAGCATTGAAAAGTGGAAAGGTTGAAGGTGCTGTAGTTGAA
    AAACCTGTAGCAGAAGCATATTTAAAACAAAATCCTAAATTAGGAATTTCCAATGTGAAA
    TTTAATGAAGAAGAAAAAGATACAGTGATAGCAGTGCCAAAAGATTCACCAAAATTATTG
    TCACAAATTAATAAAACGATTAAGGAGGTTAAAGATAAAGGATTAATCGATAAATATATG
    ACTAATGCTGCAAATGCGATGAATGATGACAGTGGTTTTATTTCTAAGTATGGTAGTTTT
    TTCTTGAAAGGAATTAAGATTACAATATTAATTTCACTTATCGGTGTTGCATTAGGTTCT
    ATTTTAGGTGCATTCGTTGCGTTAATGAAATTAAGTAAAATAAAAATTATTTCATGGATT
    GCTTCTATCTATATCGAAATATTAAGAGGAACACCAATGTTAGTTCAAGTATTTATCGTA
    TTCTTTGGTATAACTGCTGCATTAGGACTAGACATTTCAGCTCTTGTTTGTGGAACAATT
    GCATTAGTTATTAATTCCTCAGCTTACATTGCTGAAATTATTCGTGCAGGTATAAATGCT
    GTTGATAAAGGCCAAATGGAAGCCGCACGTAGTCTAGGTTTGAATTATAGACAAACGATG
    AAAAGTGTAATTATGCCACAAGCAATTAAAAATATTTTACCAGCTTTAGGTAATGAATTT
    GTCACTTTAATTAAAGAATCATCTATTGTGTCAACAATTGGAGTTGGCGAAATTATGTTT
    AATGCACAAGTGGTTCAAGGTATTTCATTTGACCCATTCACACCATTAATAGTGGCAGCA
    GCATTATACTTTGTCTTAACATTTGTACTTACACGCATTATGAACATGATTGAAGGGAGA
    TTGAATGCCAGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1458

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01991
  • symbol: SAOUHSC_01991
  • description: ABC transporter permease
  • length: 485
  • theoretical pI: 9.56041
  • theoretical MW: 53122.2
  • GRAVY: 0.212371

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 159)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 153.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and amineslysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 136.6)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 98.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 59.7)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.9)
    extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 41.1)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 29.8)
    MetabolismEnergy metabolismOtherpca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Signal transductionRegulatory functionsDNA interactionspca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.7)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 18.2)
    Hypothetical proteinsConservedconserved hypothetical protein (TIGR02285; HMM-score: 14.5)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 11.9)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 11.6)
  • TheSEED:  
    Arginine ABC transporter, substrate-binding and permease protein 
  • PFAM:
    PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 190.2)
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 85.5)
    PBP (CL0177) Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 37.8)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 16.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.74
    • Ymax_pos: 29
    • Cmax: 0.743
    • Cmax_pos: 29
    • Smax: 0.922
    • Smax_pos: 16
    • Smean: 0.763
    • D: 0.749
  • predicted transmembrane helices (TMHMM): 3

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELRVGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTGKIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKGTEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISNVKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISKYGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPMLVQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSLGLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISFDPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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