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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01617
  • pan locus tag?: SAUPAN004075000
  • symbol: SAOUHSC_01617
  • pan gene symbol?: argR
  • synonym:
  • product: arginine repressor

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01617
  • symbol: SAOUHSC_01617
  • product: arginine repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 1541182..1541634
  • length: 453
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    GTGCCCAAAAAATCGGTTAGGCATATAAAAATTAGAGAAATTATTTCAAATGAACAGATA
    GAGACACAAGATGAATTAGTTAAACGATTAAACGATTATGATTTAAATGTCACTCAAGCA
    ACTGTTTCTCGTGATATTAAAGAACTACAACTTATTAAAGTACCTATACCTTCAGGTCAA
    TATGTTTATAGTTTACCAAATGATAGAAAATTCCATCCTTTAGAAAAATTGGGACGTTAT
    TTAATGGATTCCTTTGTTAATATAGATGGTACTGATAATTTACTTGTTCTAAAAACATTA
    CCTGGTAATGCACAATCTATTGGAGCTATATTAGACCAAATCAATTGGGAAGAAGTACTA
    GGCACAATTTGTGGTGATGATACTTGTTTAATTATTTGTCGAAGCAAAGAGGCAAGTGAT
    GAAATCAAGTCAAGAATTTTCAATTTGTTATAA
    60
    120
    180
    240
    300
    360
    420
    453

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01617
  • symbol: SAOUHSC_01617
  • description: arginine repressor
  • length: 150
  • theoretical pI: 5.28159
  • theoretical MW: 17097.6
  • GRAVY: -0.267333

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyarginine repressor (TIGR01529; HMM-score: 169.8)
    Signal transductionRegulatory functionsDNA interactionsarginine repressor (TIGR01529; HMM-score: 169.8)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Arginine pathway regulatory protein ArgR, repressor of arg regulon 
    Arginine; urea cycle, polyaminesArginine Biosynthesis extended Arginine pathway regulatory protein ArgR, repressor of arg regulon 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Arginine pathway regulatory protein ArgR, repressor of arg regulon 
  • PFAM:
    HTH (CL0123) Arg_repressor; Arginine repressor, DNA binding domain (PF01316; HMM-score: 94.5)
    no clan definedArg_repressor_C; Arginine repressor, C-terminal domain (PF02863; HMM-score: 92.4)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 17)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.138
    • Ymax_pos: 11
    • Cmax: 0.107
    • Cmax_pos: 24
    • Smax: 0.344
    • Smax_pos: 1
    • Smean: 0.151
    • D: 0.143
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSRDIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPGNAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01617 (ArgR*)
    ArgR* (TF) important in Arginine biosynthesis, Arginine degradationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]