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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01613
  • pan locus tag?: SAUPAN004072000
  • symbol: SAOUHSC_01613
  • pan gene symbol?: bfmBAA
  • synonym:
  • product: 2-oxoisovalerate dehydrogenase, E1 component subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01613
  • symbol: SAOUHSC_01613
  • product: 2-oxoisovalerate dehydrogenase, E1 component subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1536907..1537899
  • length: 993
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTGATTATAAATCATTAGGCCTTAGCGAAGAAGACCTAAAAGTAATATATAAATGG
    ATGGATTTAGGTAGAAAAATAGATGAAAGACTGTGGTTATTGAACCGTGCAGGTAAAATT
    CCATTTGTTGTAAGTGGTCAAGGACAAGAAGCAACTCAGATTGGGATGGCATACGCATTA
    GAAGAAGGTGATATTACAGCGCCTTATTATAGAGATCTAGCTTTCGTTACTTATATGGGT
    ATCTCAGCGTATGATACGTTTTTATCAGCGTTTGGAAAGAAAGACGATGTTAACTCTGGT
    GGAAAACAAATGCCATCACACTTTAGTAGTAGAGCTAAGAATATTTTGTCTCAAAGTTCA
    CCGGTAGCAACTCAAATACCCCATGCTGTTGGTGCTGCTTTAGCTTTAAAAATGGATGGT
    AAGAAGAAAATTGCAACAGCAACTGTTGGTGAAGGTAGCTCAAACCAAGGTGACTTCCAT
    GAAGGTTTAAACTTCGCGGGTGTACACAAACTTCCTTTTGTATGTGTCATTATAAATAAT
    AAATATGCAATTTCAGTACCTGATTCATTACAATACGCAGCTGAAAAATTATCGGATAGA
    GCACTTGGTTACGGTATACATGGTGAACAAGTAGATGGTAATGATCCATTAGCAATGTAT
    AAAGCGATGAAAGAAGCAAGAGATCGAGCTATTTCTGGTCAAGGTTCAACTTTGATTGAA
    GCAGTAACAAGTCGTATGACAGCTCATTCATCAGATGATGACGACCAATATCGTACGAAA
    GAAGAGCGTGAAACGCTTAAAAAAGCAGACTGCAATGAAAAGTTCAAAAAAGAGTTGCTT
    TCAGCTGGCATTATCGATGATGCTTGGTTGGCAGAAATAGAAGCGGAGCATAAAGACATC
    ATCAATAAAGCGACAAAGGCTGCTGAAGACGCGCCATATCCAAGTGTCGAAGAAGCCTAT
    GCATTTGTGTACGAAGAAGGGAGTCTTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    993

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01613
  • symbol: SAOUHSC_01613
  • description: 2-oxoisovalerate dehydrogenase, E1 component subunit alpha
  • length: 330
  • theoretical pI: 4.94909
  • theoretical MW: 36229.5
  • GRAVY: -0.399091

Function[edit source | edit]

  • reaction:
    EC 1.2.4.4?  ExPASy
    3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 316.8)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 223.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
    MetabolismEnergy metabolismTCA cycleoxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 10.9)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsIsoleucine degradation Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) 
    Branched-chain amino acidsValine degradation Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) 
    CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) 
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 282.4)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 24)
    DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.7)
    XFP_N; XFP N-terminal domain (PF09364; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 60
    • Cmax: 0.215
    • Cmax_pos: 60
    • Smax: 0.146
    • Smax_pos: 47
    • Smean: 0.07
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVVSGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGKQMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFHEGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLAMYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFKKELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]