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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00148
  • pan locus tag?: SAUPAN001023000
  • symbol: argJ
  • pan gene symbol?: argJ
  • synonym:
  • product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00148
  • symbol: argJ
  • product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 158734..159975
  • length: 1242
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAACATCAAGAAACGACATCACAACAATATAACTTTTCAATTATTAAACATGGCGAT
    ATCAGTACACCTCAAGGCTTCACGGCTGGTGGTATGCACATCGGTTTACGCGCTAACAAA
    AAAGACTTTGGGTGGATTTACTCATCGTCTTTGGCAAGTGCAGCTGCCGTATATACTTTA
    AATCAGTTTAAAGCTGCACCACTTATTGTCACTGAAGACACTTTACAAAAGTCTAAAGGA
    AAATTACAAGCACTTGTTGTTAATTCAGCTAATGCAAATTCTTGTACCGGTCAACAAGGC
    ATAGATGATGCACGACAAACACAAACATGGGTTGCTCAACAACTTCAAATACCATCTGAG
    CATGTTGCTGTTGCTTCAACTGGGGTCATTGGTGAATATTTGCCTATGGATAAAATTAAG
    ACTGGGACCGAACATATTAAGGATGCTAATTTTGCAACGCCAGGTGCGTTTAACGAGGCA
    ATTTTAACAACTGATACCTGTACAAAACATATCGCTGTATCACTAAAAATCGATGGTAAA
    ACCGTTACAATTGGTGGTAGCACCAAAGGTTCAGGTATGATTCACCCAAATATGGCTACC
    ATGCTTGCTTTTATAACAACCGATGCATCGATTGAATCGAATACACTTCATCAATTATTA
    AAATCTTCGACTGACCATACATTTAATATGATTACTGTTGATGGCGATACAAGTACAAAT
    GACATGGTATTAGTCATGGCAAATCACCAAGTTGAACACCAAATACTTAGTCAAGACCAT
    CCACAATGGGAAACATTTGTTGATGCATTCAATTTTGTCTGTACATTTTTAGCTAAAGCT
    ATAGCCAGAGATGGCGAAGGCGCAACAAAGTTAATCAGCGTCAATGTGTCAGGCGCTAAA
    TCAATATCAGATGCAAGAAAAATCGGTAAAACTATCGTAAGTTCAAATCTAGTAAAATCA
    GCTATTTTTGGCGAAGATGCCAATTTTGGTCGAATCATTACAGCTATTGGCTACAGCGGA
    TGTGAAATTGATCCTAACTGCACATATGTTCAACTGAACCAAATACCTGTCGTTGATAAA
    GGTATGGCTGTACTATTTGATGAGCAAGCTATGTCGAATACATTAACTCATGAAAATGTC
    ACAATTGACGTTCAGCTTGGTTTAGGTAACGCTGCAGCGACTGCATACGGTTGTGATTTA
    TCCTATGATTATGTGCGTATCAACGCATCATATCGAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1242

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00148
  • symbol: ArgJ
  • description: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
  • length: 413
  • theoretical pI: 6.18088
  • theoretical MW: 44399.8
  • GRAVY: -0.108232

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.1?  ExPASy
    Amino-acid N-acetyltransferase Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
    EC 2.3.1.35?  ExPASy
    Glutamate N-acetyltransferase N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamate N-acetyltransferase/amino-acid acetyltransferase (TIGR00120; EC 2.3.1.1,2.3.1.35; HMM-score: 494.5)
  • TheSEED  :
    • Glutamate N-acetyltransferase (EC 2.3.1.35)
    • N-acetylglutamate synthase (EC 2.3.1.1)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Glutamate N-acetyltransferase (EC 2.3.1.35)
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  N-acetylglutamate synthase (EC 2.3.1.1)
  • PFAM:
    DmpA_ArgJ (CL0635) ArgJ; ArgJ family (PF01960; HMM-score: 481.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.67
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027663
    • TAT(Tat/SPI): 0.010515
    • LIPO(Sec/SPII): 0.002982
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFGWIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGIDDARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEAILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQLLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTFLAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIITAIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAAATAYGCDLSYDYVRINASYRT

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ArgR* (repression) regulon
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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