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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1857 [new locus tag: NWMN_RS10670 ]
  • pan locus tag?: SAUPAN004954000
  • symbol: NWMN_1857
  • pan gene symbol?: ppaC
  • synonym:
  • product: putative manganese-dependent inorganic pyrophosphatase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1857 [new locus tag: NWMN_RS10670 ]
  • symbol: NWMN_1857
  • product: putative manganese-dependent inorganic pyrophosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 2069875..2070810
  • length: 936
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    TTGACAATGGCTAAAACATATATTTTCGGACATAAGAATCCAGACACTGATGCAATTTCA
    TCTGCGATTATTATGGCAGAATTTGAACAACTTCGAGGTAATTCAGGAGCCAAAGCATAC
    CGTTTAGGTGATGTGAGTGCAGAAACTCAATTCGCGTTAGATACATTTAATGTACCTGCT
    CCGGAATTATTAACAGATGATTTAGATGGTCAAGATGTTATCTTAGTTGATCATAACGAA
    TTCCAACAAAGTTCTGATACGATTGCCTCTGCTACAATTAAGCATGTAATTGATCATCAC
    AGAATTGCAAATTTCGAAACTGCTGGTCCTTTATGTTATCGTGCTGAACCAGTTGGTTGT
    ACAGCTACAATTTTATACAAAATGTTTAGAGAACGTGGCTTTGAAATTAAACCTGAAATT
    GCCGGTTTAATGTTATCAGCAATTATCTCAGATAGCTTACTTTTCAAATCACCAACATGT
    ACACAACAAGATGTTAAAGCAGCTGAAGAATTAAAAGATATTGCTAAAGTTGATATTCAA
    AAGTACGGCTTAGATATGTTAAAAGCAGGTGCTTCAACAACTGATAAATCAGTTGAATTC
    TTATTAAACATGGATGCTAAATCATTTACTATGGGTGACTATGTGACTCGTATTGCACAA
    GTTAATGCTGTTGACCTTGACGAAGTGTTAAATCGTAAAGAAGATTTAGAAAAAGAAATG
    TTAGCTGTAAGTGCACAAGAAAAATATGACTTATTTGTACTTGTTGTTACTGACATCATT
    AATAGTGATTCTAAAATTTTAGTTGTAGGTGCTGAAAAAGATAAAGTTGGCGAAGCATTC
    AATGTTCAATTAGAAGATGACATGGCCTTCTTATCTGGTGTCGTTTCTCGAAAAAAACAA
    ATCGTACCTCAAATCACTGAAGCATTAACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1857 [new locus tag: NWMN_RS10670 ]
  • symbol: NWMN_1857
  • description: putative manganese-dependent inorganic pyrophosphatase
  • length: 311
  • theoretical pI: 4.45124
  • theoretical MW: 34300.9
  • GRAVY: -0.1

Function[edit source | edit]

  • reaction:
    EC 3.6.1.1?  ExPASy
    Inorganic diphosphataseDiphosphate + H2O = 2 phosphate
  • TIGRFAM:
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryPhosphate metabolism Manganese-dependent inorganic pyrophosphatase (EC 3.6.1.1) 
  • PFAM:
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 99.8)
    no clan definedDHHA2; DHHA2 domain (PF02833; HMM-score: 69.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 40
    • Cmax: 0.267
    • Cmax_pos: 40
    • Smax: 0.179
    • Smax_pos: 34
    • Smean: 0.101
    • D: 0.147
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTMAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVSAETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIANFETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQQDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQVNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGEAFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]