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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1735 [new locus tag: SA_RS09960 ]
  • pan locus tag?: SAUPAN004954000
  • symbol: SA1735
  • pan gene symbol?: ppaC
  • synonym:
  • product: manganese-dependent inorganic pyrophosphatase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1735 [new locus tag: SA_RS09960 ]
  • symbol: SA1735
  • product: manganese-dependent inorganic pyrophosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 1987356..1988285
  • length: 930
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCTAAAACATATATTTTCGGACATAAGAATCCAGACACTGATGCAATTTCATCTGCG
    ATTATTATGGCAGAATTTGAACAACTTAGAGGTAATTCAGGAGCCAAAGCATACCGTTTA
    GGTGATGTGAGTGCAGAAACTCAATTCGCGTTAGATACATTTAATGTACCTGCTCCGGAA
    TTATTAACGGATGATTTAGATGGTCAAGATGTTATCTTAGTTGATCATAACGAATTCCAA
    CAAAGTTCTGATACGATTGCCTCTGCTACAATTAAGCATGTAATTGATCATCACAGAATT
    GCAAATTTCGAAACTGCTGGCCCTTTATGTTATCGTGCTGAACCAGTTGGTTGTACAGCT
    ACAATTTTATACAAAATGTTTAGAGAACGTGGCTTTGAAATTAAACCTGAAATTGCCGGT
    TTAATGTTATCAGCAATTATCTCAGATAGCTTACTTTTCAAATCACCAACATGTACACAA
    CAAGACGTTAAAGCAGCTGAAGAATTAAAAGATATTGCTAAAGTTGATATTCAAAAGTAC
    GGCTTAGATATGTTAAAAGCAGGTGCTTCAACAACTGATAAATCAGTTGAATTCTTATTA
    AACATGGATGCTAAATCATTTACTATGGGTGACTATGTGACTCGTATTGCACAAGTTAAT
    GCTGTTGACCTTGACGAAGTGTTAAATCGTAAAGAAGATTTAGAAAAAGAAATGTTAGCT
    GTAAGTGCACAAGAAAAATATGACTTATTTGTACTTGTTGTTACTGACATCATTAATAGT
    GATTCTAAAATTTTAGTTGTAGGTGCTGAAAAAGATAAAGTCGGCGAAGCATTCAATGTT
    CAATTAGAAGATGACATGGCCTTCTTATCTGGTGTTGTTTCTCGAAAAAAACAAATCGTA
    CCTCAAATCACTGAAGCATTAACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    930

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1735 [new locus tag: SA_RS09960 ]
  • symbol: SA1735
  • description: manganese-dependent inorganic pyrophosphatase
  • length: 309
  • theoretical pI: 4.45124
  • theoretical MW: 34068.6
  • GRAVY: -0.104531

Function[edit source | edit]

  • reaction:
    EC 3.6.1.1?  ExPASy
    Inorganic diphosphataseDiphosphate + H2O = 2 phosphate
  • TIGRFAM:
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryPhosphate metabolism Manganese-dependent inorganic pyrophosphatase (EC 3.6.1.1) 
  • PFAM:
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 99.9)
    no clan definedDHHA2; DHHA2 domain (PF02833; HMM-score: 69.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.181
    • Ymax_pos: 38
    • Cmax: 0.276
    • Cmax_pos: 38
    • Smax: 0.184
    • Smax_pos: 32
    • Smean: 0.092
    • D: 0.146
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVSAETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIANFETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQQDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQVNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGEAFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]