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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1928 [new locus tag: NWMN_RS11125 ]
  • pan locus tag?: SAUPAN005192000
  • symbol: lukS
  • pan gene symbol?: lukH
  • synonym:
  • product: leukocidin/hemolysin toxin subunit S

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1928 [new locus tag: NWMN_RS11125 ]
  • symbol: lukS
  • product: leukocidin/hemolysin toxin subunit S
  • replicon: chromosome
  • strand: -
  • coordinates: 2134378..2135433
  • length: 1056
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAATAAAAAACGTGTTTTAATAGCGTCATCATTATCATGTGCAATTTTATTGTTA
    TCAGCAGCAACGACTCAAGCAAATTCAGCTCATAAAGACTCTCAAGACCAAAATAAGAAA
    GAACATGTTGATAAGTCTCAACAAAAAGACAAACGTAATGTTACTAATAAAGATAAAAAT
    TCAACAGCACCGGATGATATTGGGAAAAACGGTAAAATCACAAAACGAACTGAAACAGTA
    TATGATGAGAAAACAAATATACTCCAAAATTTACAATTCGACTTTATCGATGATCCAACT
    TATGACAAGAATGTATTACTTGTTAAAAAACAAGGCTCAATTCATTCAAATTTAAAGTTT
    GAATCTCATAAAGAAGAAAAAAATTCAAATTGGTTAAAGTATCCAAGTGAGTACCATGTA
    GATTTTCAAGTAAAAAGAAATCGTAAAACTGAAATATTAGACCAATTGCCGAAAAATAAA
    ATTTCAACTGCAAAAGTAGACAGTACATTTTCATATAGCTCAGGTGGTAAATTCGATTCA
    ACAAAAGGTATTGGACGAACTTCATCAAATAGCTACTCCAAAACGATTAGTTATAATCAG
    CAAAATTATGACACAATTGCCAGCGGTAAAAATAATAACTGGCATGTACACTGGTCAGTT
    ATTGCGAATGACTTGAAGTATGGTGGAGAAGTGAAAAATAGAAATGATGAATTATTATTC
    TATAGAAATACGAGAATTGCTACTGTAGAAAACCCTGAACTAAGCTTTGCTTCAAAATAT
    AGATACCCAGCATTAGTAAGAAGTGGCTTTAATCCAGAATTTTTAACTTATTTATCTAAT
    GAAAAGTCAAATGAGAAAACGCAATTTGAAGTAACATACACACGAAATCAAGATATTTTG
    AAAAACAGACCTGGAATACATTATGCACCTCCAATTTTAGAAAAAAATAAAGATGGTCAA
    AGATTAATTGTCACTTATGAAGTTGATTGGAAAAATAAAACAGTTAAAGTCGTTGATAAA
    TATTCTGATGACAATAAACCTTATAAAGAAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

Protein[edit source | edit]

Protein Data Bank: 5K59

General[edit source | edit]

  • locus tag: NWMN_1928 [new locus tag: NWMN_RS11125 ]
  • symbol: LukS
  • description: leukocidin/hemolysin toxin subunit S
  • length: 351
  • theoretical pI: 9.95044
  • theoretical MW: 40433.8
  • GRAVY: -1.05242

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancebeta-channel forming cytolysin (TIGR01002; HMM-score: 190)
  • TheSEED:  
    Cytolytic pore-forming protein => Leukocidin LukS-H 
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 250.6)
    no clan definedGRP; Glycine rich protein family (PF07172; HMM-score: 14.3)
    Omega_toxin (CL0083) Conotoxin; Conotoxin (PF02950; HMM-score: 11.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: TTQANSAH
  • SignalP: Signal peptide length 33 aa
    • Ymax: 0.745
    • Ymax_pos: 33
    • Cmax: 0.627
    • Cmax_pos: 33
    • Smax: 0.987
    • Smax_pos: 14
    • Smean: 0.934
    • D: 0.818
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVDKSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYDKNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNKISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHWSVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLTYLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKTVKVVDKYSDDNKPYKEG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR
    SaeR (TF) important in Virulence  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]