NCBI: 10-JUN-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2006 [new locus tag: SACOL_RS10480 ]
- pan locus tag?: SAUPAN005192000
- symbol: SACOL2006
- pan gene symbol?: lukH
- synonym:
- product: Aerolysin/leukocidin family protein
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SACOL2006 [new locus tag: SACOL_RS10480 ]
- symbol: SACOL2006
- product: Aerolysin/leukocidin family protein
- replicon: chromosome
- strand: -
- coordinates: 2065994..2067049
- length: 1056
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 3238231 NCBI
- RefSeq: YP_186828 NCBI
- MicrobesOnline: 913482 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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1021ATGAAAAATAAAAAACGTGTTTTAATAGCGTCATCATTATCATGTGCAATTTTATTGTTA
TCAGCAGCAACGACTCAAGCAAATTCAGCTCATAAAGACTCTCAAGACCAAAATAAGAAA
GAACATGTTGATAAGTCTCAACAAAAAGACAAACGTAATGTTACTAATAAAGATAAAAAT
TCAACAGCACCGGATGATATTGGGAAAAACGGTAAAATCACAAAACGAACTGAAACAGTA
TATGATGAGAAAACAAATATACTCCAAAATTTACAATTCGACTTTATCGATGATCCAACT
TATGACAAGAATGTATTACTTGTTAAAAAACAAGGCTCAATTCATTCAAATTTAAAGTTT
GAATCTCATAAAGAAGAAAAAAATTCAAATTGGTTAAAGTATCCAAGTGAGTACCATGTA
GATTTTCAAGTAAAAAGAAATCGTAAAACTGAAATATTAGACCAATTGCCGAAAAATAAA
ATTTCAACTGCAAAAGTAGACAGTACATTTTCATATAGCTCAGGTGGTAAATTCGATTCA
ACAAAAGGTATTGGACGAACTTCATCAAATAGCTACTCCAAAACGATTAGTTATAATCAG
CAAAATTATGACACAATTGCCAGCGGTAAAAATAATAACTGGCATGTACACTGGTCAGTT
ATTGCGAATGACTTGAAGTATGGTGGAGAAGTGAAAAATAGAAATGATGAATTATTATTC
TATAGAAATACGAGAATTGCTACTGTAGAAAACCCTGAACTAAGCTTTGCTTCAAAATAT
AGATACCCAGCATTAGTAAGAAGTGGCTTTAATCCAGAATTTTTAACTTATTTATCTAAT
GAAAAGTCAAATGAGAAAACGCAATTTGAAGTAACATACACACGAAATCAAGATATTTTG
AAAAACAGACCTGGAATACATTATGCACCTCCAATTTTAGAAAAAAATAAAGATGGTCAA
AGATTAATTGTCACTTATGAAGTTGATTGGAAAAATAAAACAGTTAAAGTCGTTGATAAA
TATTCTGATGACAATAAACCTTATAAAGAAGGATAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SACOL2006 [new locus tag: SACOL_RS10480 ]
- symbol: SACOL2006
- description: Aerolysin/leukocidin family protein
- length: 351
- theoretical pI: 9.95044
- theoretical MW: 40433.8
- GRAVY: -1.05242
⊟Function[edit source | edit]
- reaction:
- TIGRFAM: Cellular processesToxin production and resistancebeta-channel forming cytolysin (TIGR01002; HMM-score: 190)
- TheSEED:
- PFAM: Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 250.6)no clan definedGRP; Glycine rich protein family (PF07172; HMM-score: 14.3)Omega_toxin (CL0083) Conotoxin; Conotoxin (PF02950; HMM-score: 11.2)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.73
- Internal Helices: 0
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular Possibility: 0.17
- Signal Peptide Possibility: 1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: TTQANSAH
- SignalP: Signal peptide length 33 aa
- Ymax: 0.745
- Ymax_pos: 33
- Cmax: 0.627
- Cmax_pos: 33
- Smax: 0.987
- Smax_pos: 14
- Smean: 0.934
- D: 0.818
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVDKSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYDKNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNKISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHWSVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLTYLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKTVKVVDKYSDDNKPYKEG
⊟Experimental data[edit source | edit]
- experimentally validated: PeptideAtlas
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: SACOL2006 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator: SaeR
SaeR (TF) important in Virulence RegPrecise
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
- ↑
Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS ONE: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑
Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J. Proteome Res.: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑
Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int. J. Med. Microbiol.: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit source | edit]
Stephan Fuchs, Jan Pané-Farré, Christian Kohler, Michael Hecker, Susanne Engelmann
Anaerobic gene expression in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(11);4275-89
[PubMed:17384184] [WorldCat.org] [DOI] (P p)