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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1948 [new locus tag: NWMN_RS11240 ]
  • pan locus tag?: SAUPAN005284000
  • symbol: scrB
  • pan gene symbol?: scrB
  • synonym:
  • product: sucrose-6-phosphate hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1948 [new locus tag: NWMN_RS11240 ]
  • symbol: scrB
  • product: sucrose-6-phosphate hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2155602..2157086
  • length: 1485
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACCGAATGGACTAGAGAAGAACGTTATCAACGAATCGAGGACGTTGATACTGAGTAT
    TTTAAAACATTAAAACAACAAGTTGATCAATCAAAATTTCGTCAACAATTTCATATACAA
    CCAGAAACAGGCTTATTAAATGACCCCAACGGACTTATTTTTTATAAAGGGAAGTATTAT
    GTTTCACATCAATGGTTCCCATTAGGCGCAGTACATGGCTTAAAGTATTGGTATAACTAC
    ACGAGTGATGACTTAATAAACTTTAAAGCTGAAGGGCCAATTTTAAATCCAGATACTAAA
    TATGACAGCCATGGTGTATATAGCGGTAGCGCTTTTGAATATAACGGGCATTTATATTAT
    ATGTACACAGGAAATCATCGAGATAATCATTGGCAACGACATGCGAGTCAGATGATCGCA
    CGATTGAAAGAAGACGGTTCAGTTGAAAAGTTTCCAAAGCCAGTAATTAGCCAACAACCA
    GAAGGATATACGAGTCATTTTAGAGATCCTAAAGTTTTTAAATATGATGAGAAATATTAT
    GCAATCATTGGTGCACAAAATAATGATCAGCAAGGTCGATTATTACTTTATAATACTGAA
    GATATAATTAATTGGCATTATTTAGGTGAAATAAATACAGAGTTGGATGATTTTGGATAT
    ATGTGGGAATGCCCAGATTACTTTAATGTAGATAATCAAGATGTCATACTTATTTGTCCA
    CAAGGTATTGAACCAAAAGGCGATCAGTTCAAAAATATTTATCAAAGTGGTTATATACTT
    GGAAAGTTTGATATTGAAAAGTTAACATATGAACATGAAAATTTTGTCGAGCTTGATAAT
    GGTTTTGATTTCTATGCACCTCAAACATTTTTAGATGAAAAAGGCCGACGAGTACTAATT
    GGATGGATGGGGTTACCGGAAATCGAATATCCTACTGATAATGAAGGATGGGCCCATTGC
    TTAACAATTCCTCGTGTATTAAATGTAGAAAATGGTCAACTTAAGCAACGTCCATATCCA
    GCGTTGGAAAAATTACGTCACAATAAAGAGACAGCATTAGGCTACGCAAATAAATTTACT
    CGAAAATTACATCCGTATGAAGGTAAACAGTATGAATTAATCATAGATATTTTGGATAAT
    GATGCTACCGAAGTGTACTTTGAATTACGTACATCTAAGACTTCTTCAACATTAATTGCT
    TATAACAAGCGTGAAAATAAAATAACATTAGATCGCAGCGACAGTGGTTTATTGCCGACA
    AATGTTGAAGGTACGACGCGTAGTACGATATTAGACACGCCATTAAAGCAATTACAAATT
    TTTGTTGATACATCTAGTATAGAAATTTTCTGTAATGATGGAGAACGTGTTTTGACATCT
    CGAATTTTCCCGACAGAAGACGCACTAGGTATTAAAACATCAACAGAATCAGGGCAAGTA
    TATTTACAATTTACTAAATATGATTTAAAGGATGAACATAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1948 [new locus tag: NWMN_RS11240 ]
  • symbol: ScrB
  • description: sucrose-6-phosphate hydrolase
  • length: 494
  • theoretical pI: 5.03855
  • theoretical MW: 57927.2
  • GRAVY: -0.743117

Function[edit source | edit]

  • reaction:
    EC 3.2.1.26?  ExPASy
    Beta-fructofuranosidaseHydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
  • TIGRFAM:
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidessucrose-6-phosphate hydrolase (TIGR01322; EC 3.2.1.-; HMM-score: 613)
  • TheSEED:  
    CarbohydratesDi- and oligosaccharidesSucrose utilization Sucrose-6-phosphate hydrolase (EC 3.2.1.B3) 
  • PFAM:
    B_Fructosidase (CL0143) Glyco_hydro_32N; Glycosyl hydrolases family 32 N-terminal domain (PF00251; HMM-score: 331.7)
    Concanavalin (CL0004) Glyco_hydro_32C; Glycosyl hydrolases family 32 C terminal (PF08244; HMM-score: 82.6)
    B_Fructosidase (CL0143) Glyco_hydro_130; beta-1,4-mannooligosaccharide phosphorylase (PF04041; HMM-score: 17.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 42
    • Cmax: 0.102
    • Cmax_pos: 13
    • Smax: 0.111
    • Smax_pos: 40
    • Smean: 0.073
    • D: 0.091
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETGLLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYDSHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQPEGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDFGYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFVELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQLKQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTSKTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIFCNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: ScrR
    ScrR (TF) important in Sucrose utilizationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]