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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1949 [new locus tag: NWMN_RS11245 ]
  • pan locus tag?: SAUPAN005285000
  • symbol: scrR
  • pan gene symbol?: scrR
  • synonym:
  • product: sucrose operon repressor

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1949 [new locus tag: NWMN_RS11245 ]
  • symbol: scrR
  • product: sucrose operon repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2157235..2158185
  • length: 951
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATATATCTGATATTGCCAAATTGGCAGGCGTTTCAAAAAGTACAGTATCTAGA
    TTTTTAAATAATGGATCTGTCAGTAAAAAAACAAGTGAAAAATTAACAAGAATTATAGCA
    GAACATGACTATCAACCGAATCAATTTGCTCAAAGTTTAAGAGCGAGACAAACACACCTT
    ATTGGTGCCATTATACCTAGAATGAATTCATATGCAGTAGATGAAACAATCAAAGGATTG
    GCAAAACAATGCCAAAAATATGAATCACAATTAATTTTAAATTACACAGGTTTAAATATC
    GAAGCAGAAATACAAGCGCTTGAAACATTAGCACGCAGTAAAGTAGATGGTATTGTTTTA
    ATGGCTACAGACATAACAGAGAGACATATTGAAGTCATTAATAAAATGAATGTACCAATC
    GTTATTGTTGGTCAACAACATGAACAACTTCATAGTATTGTGCATGATGATTATAAAGCA
    GGTCAAATTATAGGCGAATGGATTGGTCAACAGGGATATCAACAAGTTGAAGTGTTTAGT
    GTAAGTGAAAAAGATATTGCAGTTGGTATACATAGAAAACGTGGTTTACTTGACCAGTTA
    GCTAAATACCAAATTAAACCTAATATACATGAAACAAATTTTACTTATGTGGAAGCACAA
    AAAGATGTTGCAAATGTTTTGGAAAATGTGGAGCAAGTAGATGCGGTTGTTGGAGCAACT
    GATACGATTGCATTAGCTGCCTATAAATATTATTCTGATAAAAAAGATGTTATGAAACCA
    CATCAAATATATGGTTTTGGTGGTGACCCAATGACACAATTAGTGTCTCCATCGATAAAA
    ACAATTCATTATAATTATTTTGAAGCTGGCCAATGCGCGATGGAAGAGATACAACAGATG
    CTTAAAAAGCAAGATATGCCATATAGCGTCACAGTAGATGTTAATATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    951

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1949 [new locus tag: NWMN_RS11245 ]
  • symbol: ScrR
  • description: sucrose operon repressor
  • length: 316
  • theoretical pI: 6.6818
  • theoretical MW: 35499.3
  • GRAVY: -0.297785

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02405; HMM-score: 150.5)
    Signal transductionRegulatory functionsDNA interactionscatabolite control protein A (TIGR01481; HMM-score: 119.5)
    Signal transductionRegulatory functionsDNA interactionsD-fructose-responsive transcription factor (TIGR02417; HMM-score: 114.2)
    CRISPR-associated endonuclease Cas1 (TIGR00287; EC 3.1.-.-; HMM-score: 15.9)
  • TheSEED:  
    CarbohydratesDi- and oligosaccharidesSucrose utilization Sucrose operon repressor ScrR, LacI family 
  • PFAM:
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 64.3)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 63.1)
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 46.7)
    Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 38.9)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 20.8)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 16.8)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 16.7)
    no clan definedVasohibin; Vasohibin (PF14822; HMM-score: 15.4)
    Cas_Cas1; CRISPR associated protein Cas1 (PF01867; HMM-score: 15.1)
    HTH (CL0123) HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 14.6)
    HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 14.5)
    DUF3853; Protein of unknown function (DUF3853) (PF12964; HMM-score: 14.3)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 14)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.9)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.7)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.3)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.9)
    6PGD_C (CL0106) NAD_binding_11; NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase (PF14833; HMM-score: 12.9)
    HTH (CL0123) HTH_29; Winged helix-turn helix (PF13551; HMM-score: 12.8)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 12.7)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12.4)
    MarR; MarR family (PF01047; HMM-score: 12.4)
    MarR_2; MarR family (PF12802; HMM-score: 12)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.212
    • Ymax_pos: 13
    • Cmax: 0.155
    • Cmax_pos: 30
    • Smax: 0.503
    • Smax_pos: 5
    • Smean: 0.443
    • D: 0.302
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]