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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2020 [new locus tag: NWMN_RS11675 ]
  • pan locus tag?: SAUPAN005407000
  • symbol: NWMN_2020
  • pan gene symbol?:
  • synonym:
  • product: Sua5/YciO/YrdC/YwlC family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2020 [new locus tag: NWMN_RS11675 ]
  • symbol: NWMN_2020
  • product: Sua5/YciO/YrdC/YwlC family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2236285..2237331
  • length: 1047
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    TTGGATACTAAAATTTGGGATGTTAGAGAATATAATGAAGATTTACAGCAATATCCTAAA
    ATTAATGAAATAAAAGACATTGTTTTAAACGGTGGTTTAATAGGTTTACCAACTGAAACA
    GTTTATGGACTTGCAGCAAATGCGACAGATGAAGAAGCTGTAGCTAAAATATATGAAGCT
    AAAGGCCGTCCATCTGACAATCCGCTTATTGTTCATATACACAGTAAAGGTCAATTAAAA
    GATTTTACATATACTTTGGATCCACGCGTAGAAAAGTTAATGCAGGCATTCTGGCCGGGC
    CCTATTTCGTTTATATTGCCGTTAAAGCTAGGCTATCTATGTCGAAAAGTTTCTGGAGGT
    TTATCATCAGTTGCTGTTAGAATGCCAAGCCATTCTGTAGGTAGACAATTATTACAAATC
    ATAAATGAACCTCTAGCTGCTCCAAGTGCTAATTTAAGTGGTAGACCTTCACCAACAACT
    TTCAATCATGTATATCAAGATTTGAATGGCCGTATCGATGGTATTGTTCAAGCTGAACAA
    AGTGAAGAAGGATTAGAAAGTACGGTTTTAGATTGCACATCTTTTCCTTATAAAATTGCA
    AGACCTGGTTCTATAACAGCAGCAATGATTACAGAAATACTTCCGAATAGTATCGCCCAT
    GCTGATTATAATGATACTGAACAGCCAATTGCACCAGGTATGAAGTATAAGCATTACTCA
    CCCAATACACCACTTACAATTATTACAGATATTGAGAGCAAAATTGGAAATGACGGTAAA
    GATTGGTCTTCTATAGCTTTTATTGTGCCGAGTAATAAGGTGGCGTTTATACCAAGTGAA
    GCGCAATTCATTCAATTATGTCAGGATGACAATGATGTTAAACAAGCAAGTCATAATCTT
    TATGATGTGTTACATTCACTTGATGAAAATGAAAATATTTCAGCGGCGTATATATACGGC
    TTTGAGCTGAATGATAATACAGAAGCAATTATGAATCGCATGTTAAAAGCTGCAGGTAAT
    CACATTATTAAAGGATGTGAACTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1047

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2020 [new locus tag: NWMN_RS11675 ]
  • symbol: NWMN_2020
  • description: Sua5/YciO/YrdC/YwlC family protein
  • length: 348
  • theoretical pI: 4.80011
  • theoretical MW: 38445.3
  • GRAVY: -0.269253

Function[edit source | edit]

  • reaction:
    EC 2.7.7.87?  ExPASy
    L-threonylcarbamoyladenylate synthaseL-threonine + ATP + HCO3(-) = L-threonylcarbamoyladenylate + diphosphate + H2O
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family (TIGR00057; HMM-score: 194.5)
    Genetic information processingProtein fateProtein modification and repaircarbamoyltransferase HypF (TIGR00143; HMM-score: 27.4)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationYrdC-YciO TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA 
    RNA processing and modificationYrdC-YciO-Sua5 and associated protein families TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA 
  • PFAM:
    no clan definedSua5_yciO_yrdC; Telomere recombination (PF01300; HMM-score: 179.4)
    GT-B (CL0113) SUA5; Putative GTP-binding controlling metal-binding (PF03481; HMM-score: 76.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 54
    • Cmax: 0.123
    • Cmax_pos: 46
    • Smax: 0.297
    • Smax_pos: 45
    • Smean: 0.107
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGLAANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPISFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTTFNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSIAHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAFIPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRMLKAAGNHIIKGCEL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]