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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2056 [new locus tag: NWMN_RS11875 ]
  • pan locus tag?: SAUPAN005492000
  • symbol: glmS
  • pan gene symbol?: glmS
  • synonym:
  • product: glucosamine--fructose-6-phosphate aminotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2056 [new locus tag: NWMN_RS11875 ]
  • symbol: glmS
  • product: glucosamine--fructose-6-phosphate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2274710..2276515
  • length: 1806
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTGTGGAATTGTTGGTTATATTGGCTATGATAATGCCAAAGAATTATTATTAAAAGGT
    TTAGAAAAATTAGAATACAGAGGTTATGACTCTGCAGGTATCGCAGTAGTAAATGATGAT
    AATACAACTGTATTTAAAGAAAAAGGTCGTATTGCAGAATTACGTAAAGTTGCTGATAGT
    AGCGATTTTGATGGACCTGTTGGAATCGGTCACACACGTTGGGCAACACACGGTGTACCG
    AATCATGAAAACTCTCATCCACATCAATCATCAAATGGCCGTTTTACTCTAGTTCATAAC
    GGTGTTATTGAAAACTATGAAGAGTTAAAAGGTGAATACTTACAAGGTGTATCATTCATT
    TCAGAAACAGATACAGAAGTTATCGTTCAATTAGTTGAATACTTTTCAAATCAAGGACTT
    TCAACTGAAGAAGCATTTACAAAAGTTGTGTCATTATTACATGGTTCATATGCATTAGGT
    TTATTAGATGCTGAAGACAAAGACACAATCTATGTTGCTAAAAATAAATCACCATTATTA
    TTAGGTGTTGGTGAAGGTTTCAATGTTATCGCATCAGACGCACTTGCAATGTTACAAGTG
    ACAAGCGAATATAAAGAAATCCATGACCATGAAATCGTTATTGTTAAAAAAGATGAAGTT
    ATTATTAAAGATGCAGATGGAAACGTTGTAGAACGTGATTCATATATTGCTGAAATTGAT
    GCATCAGATGCTGAAAAAGGTGTTTATGCACACTACATGTTAAAAGAAATTCATGAACAA
    CCAGCAGTAATGCGTCGTATTATTCAAGAATATCAAGATGCAGAAGGTAACTTGAAAATT
    GATCAAGACATCATCAATGATGTTAAAGAAGCAGACCGCATTTACGTTATTGCAGCAGGT
    ACAAGCTACCATGCAGGTTTAGTAGGTAAAGAATTTTTAGAAAAATGGGCTGGCGTACCA
    ACTGAAGTACACGTTGCATCAGAGTTTGTCTACAACATGCCATTATTATCTGAAAAACCA
    TTGTTCGTTTATATTTCTCAATCAGGTGAAACTGCAGATAGCCGCGCCGTATTAGTTGAA
    ACTAATAAATTAGGTCATAAATCATTAACAATCACTAATGTTGCAGGTTCAACTTTATCA
    CGTGAAGCAGACCACACATTGTTATTACACGCGGGTCCTGAAATCGCAGTTGCATCTACA
    AAAGCATATACTGCACAAATTGCAGTATTATCAATCTTGTCTCAAATCGTTGCAAAAGAG
    CATGGTCGTGAAGCAGATATTGATTTATTGAGAGAATTAGCAAAAGTAACAACAGCAATA
    GAAGCAATTGTTGACGATGCACCAATTATGGAACAAATTGCTACAGATTTCTTAGAAACA
    ACACGCAATGCATTCTTTATCGGACGTACTATTGACTATAACGTAAGTTTAGAAGGTGCG
    TTAAAACTTAAAGAAATTTCTTACATTCAAGCAGAAGGTTTTGCTGGTGGAGAACTTAAA
    CATGGTACAATTGCCTTAATCGAAGAAGGTACACCAGTTGTAGGTTTAGCAACACAAGAG
    AAAGTTAATTTATCAATTCGTGGTAACGTTAAAGAGGTAGTAGCACGTGGTGCACATCCA
    TGTATTATTTCTATGGAGGGTCTTGAAAAAGAAGGCGACACTTATGTCATTCCTCATGTA
    CATGAATTGTTAACGCCATTAGTATCAGTGGTTGCATTACAATTAATTTCATACTATGCA
    GCATTACACAGAGATTTAGATGTTGATAAACCACGTAACCTTGCTAAATCAGTTACTGTG
    GAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1806

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2056 [new locus tag: NWMN_RS11875 ]
  • symbol: GlmS
  • description: glucosamine--fructose-6-phosphate aminotransferase
  • length: 601
  • theoretical pI: 4.67212
  • theoretical MW: 65849
  • GRAVY: -0.0895175

Function[edit source | edit]

  • reaction:
    EC 2.6.1.16?  ExPASy
    Glutamine--fructose-6-phosphate transaminase (isomerizing)L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanglutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 749.4)
    MetabolismCentral intermediary metabolismAmino sugarsglutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 749.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisamidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 111.3)
    MetabolismAmino acid biosynthesisAspartate familyasparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 50.4)
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 39.4)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.3)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 31.8)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanN-acetylmuramic acid 6-phosphate etherase (TIGR00274; EC 4.2.1.126; HMM-score: 30.1)
    putative sugar isomerase, AgaS family (TIGR02815; HMM-score: 24.4)
    MetabolismEnergy metabolismSugarssugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 22.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsergothioneine biosynthesis protein EgtC (TIGR03442; HMM-score: 12.8)
  • TheSEED:  
    CarbohydratesOne-carbon MetabolismFormaldehyde assimilation: Ribulose monophosphate pathway Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) 
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSialic Acid Metabolism Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) 
    Cell Wall and Capsule - no subcategoryUDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) 
  • PFAM:
    SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 169.8)
    NTN (CL0052) GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 79.5)
    GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 56.2)
    GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 23.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 34
    • Cmax: 0.107
    • Cmax_pos: 47
    • Smax: 0.158
    • Smax_pos: 32
    • Smean: 0.084
    • D: 0.095
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTVFKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGVIENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALGLLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKDEVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEGNLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMPLLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAGPEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIMEQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIEEGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTPLVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]