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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2186 [new locus tag: NWMN_RS12620 ]
  • pan locus tag?: SAUPAN005749000
  • symbol: NWMN_2186
  • pan gene symbol?: ydbM
  • synonym:
  • product: acyl-CoA dehydrogenase-related protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2186 [new locus tag: NWMN_RS12620 ]
  • symbol: NWMN_2186
  • product: acyl-CoA dehydrogenase-related protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2407441..2408376
  • length: 936
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTTATTTTGCAATCATTTTTAGGCGAACTTGATGGTGCCACAGCATTATCTATTGGT
    TGGCATGTGAGTGTCGTAGGACAAATTTATGAACAGAAATTATGGTCTCAAGATATGTTG
    GAGCAATTTGCTGTTGAAATTAATAATGGTGCATTAGTTAATAGAGCAGTTAGTGAAGCT
    GAAATGGGTAGTCCAACAAGAGGGGGAAGACCAAGTACACATGCTGTTAAAGCTGATGAT
    GGGTATATTTTAAATGGTGTGAAGACATATACATCAATGAGTAAAGCACTAACACATATT
    ATTGTTGCTGCTTATATAGAAGAATTAGAAAGTGTTGGTTTTTTCTTAGTAGACAGGAAT
    TTGCCTGGTGTTGAAATTGCCGATAATTGGGACGTATTAGGTATGCGAGCGACAGAAAGT
    CATGATTTAATATTAAATGATGTAAAAGTACCATTAAAACATTTAGTGGAAACAGAGAAA
    AGTAAAGCGCCAAATGGTTGGATATTGCATATACCAAGCTGTTATTTAGGTATAGCACAA
    GCTGCTAGAAATTATGCTGTAGACTTTGCAATCCAACATAGCCCTAATAGTATTGAAGGA
    ACGATTGCAACATTGCCAACTGTGCAACAAAATTTAGGGAAAATGGAAACGCTATTGTTA
    TGTGCGAGACAATTTTTATGGAGTACAGCAAAAGGGTATCAACAATATAAAGATGACAGT
    CAGATAAGAAATCCAACAAGTGCGAGTAAAGTGATGGTAATGAATCAAGGGCTTGAAGTG
    ATTGATTTAGCGATGAGAATAGTTGGTGCTAAAAGCTTAGAAATGAATCGACCATTACAA
    CGTTACTATCGTGACATGCGTGCAGGATTACACAATCCACCTATGGAAGATGCAGCATAT
    ACAAATATTGCTAAGAGTATTACAGATACATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2186 [new locus tag: NWMN_RS12620 ]
  • symbol: NWMN_2186
  • description: acyl-CoA dehydrogenase-related protein
  • length: 311
  • theoretical pI: 5.85695
  • theoretical MW: 34413.1
  • GRAVY: -0.117685

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismCentral intermediary metabolismSulfur metabolismsulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 73)
    cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 30.9)
    pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 21.5)
  • TheSEED:  
    Acyl-CoA dehydrogenase 
  • PFAM:
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 51.7)
    no clan definedAcyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 50.6)
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 45.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 17
    • Cmax: 0.12
    • Cmax_pos: 29
    • Smax: 0.183
    • Smax_pos: 6
    • Smean: 0.153
    • D: 0.142
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVILQSFLGELDGATALSIGWHVSVVGQIYEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGVKTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLILNDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIATLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVIDLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator: NWMN_1528 (CymR*)
    CymR* (TF) important in Cysteine metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J. Bacteriol.: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog.: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)