From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2080 [new locus tag: SA_RS11955 ]
  • pan locus tag?: SAUPAN005749000
  • symbol: SA2080
  • pan gene symbol?: ydbM
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2080 [new locus tag: SA_RS11955 ]
  • symbol: SA2080
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2340324..2341478
  • length: 1155
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATGATATGGATAATTCCTTTTTAATAACAACGGAAATTCAAAGAAAATGGATTGAA
    AAATTCAAAGTAATTAGAGATACATTTAAGGCTAAAGCTGAATATAATGATCAACATAGC
    CAATTTCCATATAAAAATATTGAATGGTTAATTAAAGAAGGTTATGGAAAATTAACGTTA
    CCAAAAGCATATGGTGGTGAAGGTGCGACCATAGAAGACATGGTTATTTTGCAATCATTT
    TTAGGCGAACTTGATGGTGCCACAGCATTATCTATTGGTTGGCATGTGAGTGTCGTAGGA
    CAAATTTATGAACAGAAATTATGGTCTCAAGATATGTTGGAGCAATTTGCTGTTGAAATT
    AATAATGGTGCATTAGTTAATAGAGCAGTTAGTGAAGCTGAAATGGGTAGTCCAACAAGA
    GGGGGAAGACCAAGTACACATGCTGTTAAAGCTGATGATGGGTATATTTTAAATGGTGTG
    AAGACATATACATCAATGAGTAAAGCACTAACACATATTATTGTTGCTGCTTATATAGAA
    GAATTAGAAAGTGTTGGTTTTTTCTTAGTAGACAGGAATTTGCCTGGTGTTGAAATTGCC
    GATAATTGGGACGTATTAGGTATGCGAGCGACAGAAAGTCATGATTTAATATTAAATGAT
    GTAAAAGTACCATTAAAACATTTAGTGGAAACAGAGAAAAGTAAAGCGCCAAATGGTTGG
    ATATTGCATATACCAAGCTGTTATTTAGGTATAGCACAAGCTGCTAGAAATTATGCTGTA
    GACTTTGCAATCCAACATAGCCCTAATAGTATTGAAGGAACGATTGCAACATTGCCAACT
    GTGCAACAAAATTTAGGGAAAATGGAAACGCTATTGTTATGTGCGAGACAATTTTTATGG
    AGTACAGCAAAAGGGTATCAACAATATAAAGATGACAGTCAGATAAGAAATCCAACAAGT
    GCGAGTAAAGTGATGGTAATGAATCAAGGGCTTGAAGTGATTGATTTAGCGATGAGAATA
    GTTGGTGCTAAAAGCTTAGAAATGAATCGACCATTACAACGTTACTATCGTGACATGCGT
    GCAGGATTACACAATCCACCTATGGAAGATGCAGCATATACAAATATTGCTAAGAGTATT
    ACAGATACATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2080 [new locus tag: SA_RS11955 ]
  • symbol: SA2080
  • description: hypothetical protein
  • length: 384
  • theoretical pI: 5.85467
  • theoretical MW: 42999.8
  • GRAVY: -0.246354

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismCentral intermediary metabolismSulfur metabolismsulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 104.2)
    pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 34.3)
    cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 32.9)
  • TheSEED:  
    Acyl-CoA dehydrogenase 
  • PFAM:
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 51.2)
    no clan definedAcyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 50)
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 44.5)
    AcylCoA_ox_dh_N (CL0544) Acyl-CoA_dh_N; Acyl-CoA dehydrogenase, N-terminal domain (PF02771; HMM-score: 28)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 34
    • Cmax: 0.13
    • Cmax_pos: 34
    • Smax: 0.141
    • Smax_pos: 2
    • Smean: 0.097
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPYKNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQIYEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGVKTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLILNDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIATLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVIDLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SA1453 (CymR*)
    CymR* (TF) important in Cysteine metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog.: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)