NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2213 [new locus tag: NWMN_RS12765 ]
• pan locus tag^?: SAUPAN005790000
• symbol: NWMN_2213
• pan gene symbol^?: —
• synonym:
• product: DeoR family regulatory protein

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2213 [new locus tag: NWMN_RS12765 ]
• symbol: NWMN_2213
• product: DeoR family regulatory protein
• replicon: chromosome
• strand: +
• coordinates: 2435325..2436017
• length: 693
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331337 NCBI
• RefSeq: YP_001333247 NCBI
• MicrobesOnline: 3707815 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAATAAAGCTGAAAGGCAAAATTTAATAATTACTGCAATTCAACAAAATAAAAAAATG
  ACCGCTTTAGAATTAGCTAAATATTGCAACGTATCCAAACGCACAATTTTAAGAGATATT
  GATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTAC
  CAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCC
  TTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATC
  AACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAA
  CGCATGGACTTTTATATTAAATTTGATGACACACAACATATGACACTCCCAATGCTGTTT
  TCCGACATTTTAATTTATTGTACAGAACGAAATGTGATGTTAGTAGATCATAGGGTTGAT
  GATAATATTAAAGCTGAAAACGTTATATTTATTGGCCTTTTGTGTAAACATGGACATTGG
  CATGCAGTCATTTATGACATTGCTCAAGACAAAACTGCCGAACTCGAAATTGAAAATATT
  ATAGATATTTCGTATTCATTCGGTAAGACGATTCAAACCAGAGACATATCCATTGATAAC
  TATCATCAATTTTTAAACCCCATCGATTCCTAA
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⊟Protein

⊟General

• locus tag: NWMN_2213 [new locus tag: NWMN_RS12765 ]
• symbol: NWMN_2213
• description: DeoR family regulatory protein
• length: 230
• theoretical pI: 7.17682
• theoretical MW: 26618.5
• GRAVY: -0.268261

⊟Function

• reaction:
• TIGRFAM:
  Unknown functionGeneralRrf2 family protein (TIGR00738; HMM-score: 17.7)
  Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaD family protein (TIGR04548; HMM-score: 16.8)
  Signal transductionRegulatory functionsDNA interactionsCRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 15.5)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherFeS assembly SUF system regulator (TIGR02944; HMM-score: 15)
  Signal transductionRegulatory functionsDNA interactionsFeS assembly SUF system regulator (TIGR02944; HMM-score: 15)
  Unknown functionGeneralDNA binding domain, excisionase family (TIGR01764; HMM-score: 14)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtheriron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 13)
  Signal transductionRegulatory functionsDNA interactionsiron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 13)
  Signal transductionRegulatory functionsDNA interactionsbiotin operon repressor (TIGR00122; HMM-score: 12.1)
• TheSEED:  
  Transcriptional regulator, DeoR family 
• PFAM:
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 49.1)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 36.6)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 25.5)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 20.6)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 18)
  TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP) (PF07106; HMM-score: 16.4)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 16.3)
  HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 14.9)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13.4)
  MarR; MarR family (PF01047; HMM-score: 13.3)
  Tn7_Tnp_TnsA_C; TnsA endonuclease C terminal (PF08721; HMM-score: 12.8)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 11.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.15
  • Ymax_pos: 23
  • Cmax: 0.126
  • Cmax_pos: 23
  • Smax: 0.206
  • Smax_pos: 15
  • Smean: 0.162
  • D: 0.154
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222425 NCBI
• RefSeq: YP_001333247 NCBI
• UniProt: A0A0H3KA23 UniProt

⊟Protein sequence

• MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLENQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINAIIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVDDNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISIDNYHQFLNPIDS

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2213 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator: CcpA

  CcpA

  (TF)

  important in Carbon catabolism

  RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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