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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2232 [new locus tag: NWMN_RS12865 ]
  • pan locus tag?: SAUPAN005818000
  • symbol: hutU
  • pan gene symbol?: hutU
  • synonym:
  • product: urocanate hydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2232 [new locus tag: NWMN_RS12865 ]
  • symbol: hutU
  • product: urocanate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 2454120..2455781
  • length: 1662
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGAAAAATTCAAGCAAAAAAAGGTTTGAGTATTGAGTGTAAAGGTTGGGAACAAGAA
    GCAGTACTTAGAATGTTATACAACAATTTAGACCCAGAAGTTGCTGAAAGACCAGAAGAT
    TTAGTTGTTTATGGTGGTATTGGAAAAGCAGCACGTAATTGGGAAGCTTTTGAAGCAATA
    GAGAAAACGTTACGAGAATTAGAATCAGATGAGACAATGTTAGTACAATCAGGAAAGCCA
    GTTGCTGTATTTAAAACACATGAAGAAGCGCCACGTGTATTAATTTCAAATTCAGTATTA
    GTGCCTGAATGGGCAAACTGGGATCATTTTAATGAATTAGATAAAAAAGGTTTAATCATG
    TATGGACAGATGACAGCCGGTAGTTGGATATATATTGGTTCTCAAGGTATTGTGCAAGGT
    ACTTATGAGACATTTGCCGAGCTAGGTAATCAACACTTTAATGGAGATTTAGCAGGCACT
    GTTACACTGACAGCAGGTTTAGGTGGTATGGGTGGTGCGCAACCGTTAGCAATTACTATG
    AATCATGGGGTAGCAATTTGCGTGGATGTTGATGAAACACGTGTTGATAAGCGAATTGAT
    ACGAAATACTGTGATGTTAAAACAGCTGATTTAGATGAAGCATTAAAATTAGCAGAAGAG
    GCGAAAGAACGTGGAGAAGGATTATCAATCGGGTTAGTTGGAAATGCTGTAGACATCCAT
    CAAGCGATTCTAGAAAAAGGATTTAAAATTGACATTATTACTGACCAAACAAGTGCACAT
    GATCCGCTAAATGGATATGTGCCACAAGGATACTCTGTAGAAGAAGCGAAAGTATTGCGT
    GAAAAAGATCCGAAAAAATATGTTGAACTGTCACAAGCTTCAATGGCAAAGCATGTTGAA
    TTAATGCTTGAATTCCAAAAACGTGGCGCTGTAGCATTTGATTATGGTAACAATATTCGT
    CAAGTAGCCTTCAATAACGGAGTGAAAAATGCTTTTGACTTCCCAGGCTTTGTACCAGCT
    TACATTAGACCATTATTCTGTGAAGGTAAAGGGCCATTCCGCTTTGCTGCGTTGAGTGGT
    GATCCAAAAGATATCGAGCGTGCGGATGAAGAAATGCGCAAACTTTTCCCAGAAAATGAA
    AAGTTATTAAGATGGCTTGATTTAGCTGAAGAAAAAATTTCATATCAAGGACTACCATCA
    CGTATTGCTTGGTTAGGCTATGGAGAAAGAGCGAAAATGGGCTTAGCTTTAAATCGTCTT
    GTACGTGACGGTGAAATTTCAGCACCAATTGTTATTGGACGAGACCATTTAGATGCTGGT
    TCAGTTGCTAGTCCTAACCGTGAAACAGAAAGTATGAAAGATGGTAGTGACGCGGTTGGT
    GATTGGGCTGTATTGAATGCACTCATTAACACAGCTGCAGGTGGTTCATGGATTTCATTC
    CATCACGGTGGCGGTGTTGGCATGGGATATTCACTTCATGCGGGTATGGTTGTTGTAGCA
    GATGGATCAGAGCGTGCTGAAAGAAGATTGGAACGTGTATTGACGACTGACCCAGGTATG
    GGTGTTGCCCGACATGTTGATGCTGGCTATGACATCGCTATTCAAACAGCTAAAGAAAAA
    GGTATTCATATTCCAATGATTGATAAAGCAGGTGATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2232 [new locus tag: NWMN_RS12865 ]
  • symbol: HutU
  • description: urocanate hydratase
  • length: 553
  • theoretical pI: 5.03205
  • theoretical MW: 60632.4
  • GRAVY: -0.292586

Function[edit source | edit]

  • reaction:
    EC 4.2.1.49?  ExPASy
    Urocanate hydratase3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesurocanate hydratase (TIGR01228; EC 4.2.1.49; HMM-score: 969.4)
  • TheSEED:  
    Amino Acids and DerivativesHistidine MetabolismHistidine Degradation Urocanate hydratase (EC 4.2.1.49) 
  • PFAM:
    NADP_Rossmann (CL0063) Urocanase; Urocanase Rossmann-like domain (PF01175; HMM-score: 302.8)
    no clan definedUrocanase_C; Urocanase C-terminal domain (PF17392; HMM-score: 299)
    Urocanase_N; Urocanase N-terminal domain (PF17391; HMM-score: 203.8)
    TPR (CL0020) TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 12.3)
    no clan definedExonuc_VII_S; Exonuclease VII small subunit (PF02609; HMM-score: 11.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: NAD+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 11
    • Cmax: 0.135
    • Cmax_pos: 60
    • Smax: 0.217
    • Smax_pos: 1
    • Smean: 0.13
    • D: 0.124
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVYGGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVPEWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGTVTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLAEEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEAKVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDFPGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEKISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETESMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAERRLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: NWMN_2233 (HutR*), CcpA
    HutR* (TF) important in Histidine utilization  RegPrecise
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]