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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2272 [new locus tag: NWMN_RS13075 ]
  • pan locus tag?: SAUPAN005886000
  • symbol: NWMN_2272
  • pan gene symbol?:
  • synonym:
  • product: zinc-containing alcohol dehydrogenase superfamily protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2272 [new locus tag: NWMN_RS13075 ]
  • symbol: NWMN_2272
  • product: zinc-containing alcohol dehydrogenase superfamily protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2496237..2497241
  • length: 1005
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGATAGAAACATTTAAAGCGTTTGTAATTGATAAAGATGAGAGTGGTAAAGTGACACCA
    ACTTTCAAACAATTATCGCCTACTGATTTACCTAAAGGAGATGTGCTGATTAAAGTACAT
    TACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGATCATAATGCAGTCGTAAAA
    TCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATTGTTGAATCCGAAGCACCA
    GGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGACCTAGGTGTCAGCCATTAT
    GGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATTATCAAGCTTCCTGATACT
    TTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTATACTGCCGGTTTAGCAATT
    GAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCTGTACTCGTTCGCGGTGCT
    TCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAACTTGGTTATAAAGTTATC
    GCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAACTTGGTGCCAAAGAAGTT
    ATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTCGCATCATCAACTTGGCAA
    GCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTTACAAAGCGTTTAAATCAT
    AGTGGGTCAATTGCAGTTATTGGTATGACTGCCGGTAATACTTATACTAATTCTGTATTC
    CCTCACATTTTAAGAGGTGTAAACATTTTAGGAATTGACTCGGTATTTACTGCTATGAAA
    TTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAATGCCTGAAAATTTACATGAG
    ATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTTAACAAAGTAATTAAACAT
    GAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2272 [new locus tag: NWMN_RS13075 ]
  • symbol: NWMN_2272
  • description: zinc-containing alcohol dehydrogenase superfamily protein
  • length: 334
  • theoretical pI: 5.65303
  • theoretical MW: 36569.7
  • GRAVY: -0.111377

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityputative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 449.9)
    Unknown functionEnzymes of unknown specificityputative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 96.4)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 57.9)
    MetabolismEnergy metabolismFermentationzinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 38.6)
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 36.2)
    Unknown functionEnzymes of unknown specificityNDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 30.8)
    MetabolismEnergy metabolismAmino acids and aminesL-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 26.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllchlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 24.4)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 12.8)
  • TheSEED:  
    Fermentations: Lactate Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84) 
    Fermentations: Mixed acid Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84) 
    Glycerolipid and Glycerophospholipid Metabolism in Bacteria Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84) 
  • PFAM:
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 46.8)
    GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 34.3)
    NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 15.5)
    GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 13.6)
    NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.125
    • Ymax_pos: 52
    • Cmax: 0.156
    • Cmax_pos: 52
    • Smax: 0.14
    • Smax_pos: 30
    • Smean: 0.096
    • D: 0.114
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_2272 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]