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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
  • pan locus tag?: SAUPAN005886000
  • symbol: SAUSA300_2317
  • pan gene symbol?:
  • synonym:
  • product: putative zinc-binding dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
  • symbol: SAUSA300_2317
  • product: putative zinc-binding dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2491405..2492307
  • length: 903
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGCTGATTAAAGTACATTACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGAT
    CATAATGCAGTCGTAAAATCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATT
    GTTGAATCCGAAGCACCAGGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGAC
    CTAGGTGTCAGCCATTATGGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATT
    ATCAAGCTTCCTGATACTTTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTAT
    ACTGCCGGTTTAGCAATTGAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCT
    GTACTCGTTCGCGGTGCTTCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAA
    CTTGGTTATAAAGTTATCGCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAA
    CTTGGTGCCAAAGAAGTTATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTC
    GCATCATCAACTTGGCAAGCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTT
    ACAAAGCGTTTAAATCATAGTGGGTCAATTGCAGTTATTGGTATGACTGCCGGTAATACT
    TATACTAATTCTGTATTCCCTCACATTTTAAGAGGTGTAAACATTTTAGGAATTGACTCG
    GTATTTACTGCTATGAAATTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAATG
    CCTGAAAATTTACATGAGATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTT
    AACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
  • symbol: SAUSA300_2317
  • description: putative zinc-binding dehydrogenase
  • length: 300
  • theoretical pI: 5.87292
  • theoretical MW: 32835.5
  • GRAVY: -0.0753333

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityputative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 422.5)
    Unknown functionEnzymes of unknown specificityputative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 89.7)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 51.3)
    MetabolismEnergy metabolismFermentationzinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 35.9)
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 35.7)
    Unknown functionEnzymes of unknown specificityNDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 25.5)
    MetabolismEnergy metabolismAmino acids and aminesL-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 25)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllchlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 18.1)
  • TheSEED:  
    Fermentations: Lactate Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84) 
    Fermentations: Mixed acid Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84) 
    Glycerolipid and Glycerophospholipid Metabolism in Bacteria Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84) 
  • PFAM:
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 47.1)
    GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 30.1)
    NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 15.7)
    ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.134
    • Ymax_pos: 18
    • Cmax: 0.156
    • Cmax_pos: 18
    • Smax: 0.192
    • Smax_pos: 42
    • Smean: 0.117
    • D: 0.127
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_2317 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]