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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2324 [new locus tag: NWMN_RS13375 ]
  • pan locus tag?: SAUPAN005965000
  • symbol: bioB
  • pan gene symbol?: bioB
  • synonym:
  • product: biotin synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2324 [new locus tag: NWMN_RS13375 ]
  • symbol: bioB
  • product: biotin synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2552810..2553817
  • length: 1008
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAA
    ATTTATGAGGATACTAATATTGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGA
    AAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTTTAAATGCTAAAAGTGGTATA
    TGTCCTGAAAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
    GCTTTAATTCCAGAGGAACAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATT
    GGAACATATTGTATTGTTATGAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATT
    AGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAACTAAAAATCTGTGCATGTTTA
    GGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
    CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAA
    GATAGAACAGATACGATAGAACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTG
    ATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATATGGCATTTGCTTTAAAAGAA
    ATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
    AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATC
    AATCCTACGAAAGAAATTCGTATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAG
    CCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTGATTATCTAATTACTGGTGGG
    CAACCGAACCAATTAGACTACGATATGATTAATGATTTAGGCTTTGAAATTGATTATGAC
    ACTTGTGAAAATAAGGAAAACAAGAATGAAGTTTCAAGAGCAAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2324 [new locus tag: NWMN_RS13375 ]
  • symbol: BioB
  • description: biotin synthase
  • length: 335
  • theoretical pI: 6.7726
  • theoretical MW: 37558.7
  • GRAVY: -0.444179

Function[edit source | edit]

  • reaction:
    EC 2.8.1.6?  ExPASy
    Biotin synthaseDethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinbiotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 377.8)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster radical SAM maturase HydE (TIGR03956; HMM-score: 85.4)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis radical SAM protein (TIGR03910; HMM-score: 80.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 55.1)
    Genetic information processingProtein fateProtein modification and repair5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    MetabolismEnergy metabolismMethanogenesis5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersLipoatelipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 44.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneputative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 41.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonedehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 35.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 31)
    Hypothetical proteinsConservedradical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 28.7)
    Unknown functionEnzymes of unknown specificityradical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 23.3)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 19.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinprobable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 18.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit (TIGR03550; EC 2.5.1.77; HMM-score: 18.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxinepyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 15.9)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 13.1)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 12.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis Biotin synthase (EC 2.8.1.6) 
  • PFAM:
    no clan definedBATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 57.3)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 57.1)
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [2Fe-2S] cluster, [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 11
    • Cmax: 0.108
    • Cmax_pos: 21
    • Smax: 0.187
    • Smax_pos: 4
    • Smean: 0.149
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRAN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: BirA
    BirA (TF) important in Biotin biosynthesis  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]