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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02714
  • pan locus tag?: SAUPAN005965000
  • symbol: SAOUHSC_02714
  • pan gene symbol?: bioB
  • synonym:
  • product: biotin synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02714
  • symbol: SAOUHSC_02714
  • product: biotin synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2494978..2495985
  • length: 1008
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAA
    ATTTATGAGGATACTAATATTGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGA
    AAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTTTAAATGCTAAAAGTGGTATA
    TGTCCTGAAAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
    GCTTTAATTCCAGAGGAACAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATT
    GGAACATATTGTATTGTTATGAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATT
    AGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAACTAAAAATCTGTGCATGTTTA
    GGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
    CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAA
    GATAGAACAGATACGATAGAACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTG
    ATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATATGGCATTTGCTTTAAAAGAA
    ATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
    AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATC
    AATCCTACGAAAGAAATTCGTATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAG
    CCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTGATTATCTAATTACTGGTGGG
    CAACCGAACCAATTAGACTACGATATGATTAATGATTTAGGCTTTGAAATTGATTATGAC
    ACTTGTGAAAATAAGGAAAACAAGAATGAAGTTTCAAGAGCAAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02714
  • symbol: SAOUHSC_02714
  • description: biotin synthase
  • length: 335
  • theoretical pI: 6.7726
  • theoretical MW: 37558.7
  • GRAVY: -0.444179

Function[edit source | edit]

  • reaction:
    EC 2.8.1.6?  ExPASy
    Biotin synthaseDethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinbiotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 377.8)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster radical SAM maturase HydE (TIGR03956; HMM-score: 85.4)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis radical SAM protein (TIGR03910; HMM-score: 80.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 55.1)
    Genetic information processingProtein fateProtein modification and repair5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    MetabolismEnergy metabolismMethanogenesis5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersLipoatelipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 44.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneputative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 41.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonedehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 35.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 31)
    Hypothetical proteinsConservedradical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 28.7)
    Unknown functionEnzymes of unknown specificityradical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 23.3)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 19.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinprobable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 18.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit (TIGR03550; EC 2.5.1.77; HMM-score: 18.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxinepyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 15.9)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 13.1)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 12.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis Biotin synthase (EC 2.8.1.6) 
  • PFAM:
    no clan definedBATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 57.3)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 57.1)
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [2Fe-2S] cluster, [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 11
    • Cmax: 0.108
    • Cmax_pos: 21
    • Smax: 0.187
    • Smax_pos: 4
    • Smean: 0.149
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRAN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]