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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2503 [new locus tag: NWMN_RS14365 ]
  • pan locus tag?: SAUPAN006305000
  • symbol: NWMN_2503
  • pan gene symbol?: fdaB
  • synonym:
  • product: fructose-1,6-bisphosphate aldolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2503 [new locus tag: NWMN_RS14365 ]
  • symbol: NWMN_2503
  • product: fructose-1,6-bisphosphate aldolase
  • replicon: chromosome
  • strand: +
  • coordinates: 2749140..2750030
  • length: 891
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATAAAGAGCAATTAGAAAAAATGAAAAATGGAAAAGGCTTTATTGCCGCATTAGAC
    CAAAGTGGTGGTAGTACACCAAAAGCACTTAAAGAATATGGTGTAAACGAAGATCAATAT
    AGCAATGAAGACGAAATGTTCCAACTTGTTCACGATATGCGTACACGTGTGGTAACTTCA
    CCTTCATTCTCACCAGATAAAATTTTGGGTGCTATTCTTTTCGAACAAACAATGGATCGC
    GAAGTAGAAAGCAAATACACTGCAGATTACTTAGCTGATAAAGGTGTTGTTCCGTTCTTA
    AAAGTAGACAAAGGTCTTGCTGAAGAGCAAAATGGTGTTCAATTAATGAAACCAATCGAC
    AACTTAGACAATTTATTAGACCGTGCAAACGAACGTCACATTTTTGGTACAAAAATGCGT
    TCTAACATTTTAGAATTAAATGAGCAAGGTATCAAAGACGTTGTTGAACAACAATTTGAA
    GTTGCTAAACAAATTATTGCTAAAGGTTTAGTTCCAATTATCGAACCAGAAGTTAATATT
    AATGCAAAAGACAAAGCTGAAATTGAAAAAGTATTAAAAGCTGAACTTAAAAAAGGTTTA
    GATAGCTTAAATGCTGATCAATTAGTAATGTTGAAATTAACAATTCCTACTGAACCAAAC
    TTATACAAAGAGTTAGCTGAACATCCTAATGTTGTTCGTGTCGTTGTATTATCAGGTGGT
    TACAGCAGAGAAAAAGCAAATGAATTACTTAAAGATAACGCTGAACTTATCGCAAGTTTC
    TCACGTGCATTAGCTAGTGATTTAAGAGCTGATCAATCTAAAGAAGAATTCGATAAAGCT
    TTAGGTGATGCTGTAGAATCAATCTACGACGCATCTGTAAACAAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    891

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2503 [new locus tag: NWMN_RS14365 ]
  • symbol: NWMN_2503
  • description: fructose-1,6-bisphosphate aldolase
  • length: 296
  • theoretical pI: 4.64772
  • theoretical MW: 33054.3
  • GRAVY: -0.468243

Function[edit source | edit]

  • reaction:
    EC 4.1.2.13?  ExPASy
    Fructose-bisphosphate aldolaseD-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthertyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 11.9)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismGlycolysis and Gluconeogenesis Fructose-bisphosphate aldolase class I (EC 4.1.2.13) 
    One-carbon MetabolismFormaldehyde assimilation: Ribulose monophosphate pathway Fructose-bisphosphate aldolase class I (EC 4.1.2.13) 
  • PFAM:
    Peptidase_MH (CL0035) Glycolytic; Fructose-bisphosphate aldolase class-I (PF00274; HMM-score: 50)
    no clan definedClp_N; Clp amino terminal domain, pathogenicity island component (PF02861; HMM-score: 14.9)
    GD_AH_C; D-galactarate dehydratase / Altronate hydrolase, C terminus (PF04295; HMM-score: 12.6)
    Arginase (CL0302) Hist_deacetyl; Histone deacetylase domain (PF00850; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_0515(capD)polysaccharide biosynthesis protein CapD  [1] (data from MRSA252)
    NWMN_1096(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0960(phdB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2152(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    NWMN_2151(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2136(rplR)50S ribosomal protein L18  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1572(rplT)50S ribosomal protein L20  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_2126(rpoA)DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    NWMN_0505(rpoC)DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0357(rpsF)30S ribosomal protein S6  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_2127(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    NWMN_0507(rpsL)30S ribosomal protein S12  [1] (data from MRSA252)
    NWMN_1326(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0839fumarylacetoacetate hydrolase family protein  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)
    NWMN_1985ATP-dependent RNA helicase DEAD box family protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 11
    • Cmax: 0.109
    • Cmax_pos: 30
    • Smax: 0.369
    • Smax_pos: 2
    • Smean: 0.249
    • D: 0.196
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNEDEMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKVDKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFEVAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTEPNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEEFDKALGDAVESIYDASVNKN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]