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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS00290 [old locus tag: NWMN_0050 ]
  • pan locus tag?: SAUPAN000902000
  • symbol: NWMN_RS00290
  • pan gene symbol?:
  • synonym:
  • product: oleate hydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS00290 [old locus tag: NWMN_0050 ]
  • symbol: NWMN_RS00290
  • product: oleate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 66251..68026
  • length: 1776
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (66251..68026) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTATTACAGTTATGGAAATTATGAAGCATTTGCGCGCCCTAAAAAACCTGAAAATGTA
    GAAAACAAATCCGCTTACTTAATCGGATCTGGTCTAGCTTCACTTGCTGCAGCTTGTTTT
    TTAATAAGGGATGGTCAAATGGAAGGTTCGAAGATTCATATTTTAGAAGAGTTACCTAAA
    GCAGGTGGTAGTCTTGATGGTGAAAATATGCCTTTAAAAGGCTATGTTGTCCGCGGTGGT
    CGTGAAATGGAGAACCACTTTGAATGTTTGTGGGACTTATTCAGATCTATCCCTTCATTA
    GAAATCGATAACGCGTCTGTATTAGATGAGTTCTATTGGCTAAACAAAGAAGACCCTAAC
    TATTCTCGCTGTCGTGTTATTGAGAAACAGGGTCAACGTTTAGTCACAGACGGAGACTTC
    ACTTTGACTAAAACGGCGATTAAAGAAATTTTAGATTTATGCTTAACGAATGAAGAAGAT
    TTAGATGATGTCAAAATAACAGATGTATTTTCCGATGACTTCTTTAATTCAAACTTTTGG
    ATTTACTGGAAAACGATGTTTGCATTTGAACCGTGGCATTCTGCAATGGAAATGCGTCGC
    TATCTAATGCGATTCGTTCATCATATTAGTGGTCTCGCAGACTTTTCAGCTTTAAAATTC
    ACTAAATATAATCAATATGAATCTTTAGTATTACCTATGGTTGAATATTTAAAATCGCAT
    GGGGTTCAATTTGAATACGATGTAAAAGTCGAAGATATTAAAATAGATGTTACGACAAGT
    CAAAAAATTGCCCGAGAAATATTAATTGACCGTAATGGTAATGCAGAATCTATTAAACTG
    ACTATAAACGATCTTGTCTTTGTGACAAACGGTAGTATTACAGAAAGCTCTACTTATGGT
    GATAATGATACACCAGCGCCACCAACTGACGAATTAGGTGGTAGTTGGACACTATGGAAA
    AATTTAGCGCGACAAAGTCCTGAATTTGGTAATCCTGATAAGTTTTGCCAAAATATTCCT
    AAAAAAAGTTGGTTTGTTTCAGCAACTTCTACAACAAACAATAAAGAGATTATCGATACA
    ATAGAAAGTATTTGTAAACGTGACCCACTTGCAGGCAAAACAGTTACAGGCGGTATTATT
    ACAATCAATGATTCTGCATGGCAAATGAGTTTTACAATCAATCGTCAGCAACAGTTTAAA
    GACCAACCTGAAAATGAAATATCTACATGGATTTATGCCTTATATTCAGATGTAAACGGC
    GATTATATTAAAAAGCCAATTACAGAATGTAGTGGTAATGAAATATGCCAAGAATGGCTG
    TATCACTTAGGTGTATCAACTGACAAAATTGAAGACTTAGCAAAACATGCATCTAATACG
    ATTCCTGTTTATATGCCATATATCACATCTTATTTCATGACGCGTGCTATCGGCGACAGA
    CCTTTAGTCGTCCCGCATCAATCTCAGAACTTAGCATTTATTGGTAACTTTGCAGAAACA
    GAGCGAGACACTGTATTTACAACAGAATATTCGGTTCGTACTGCCATGGAAGCTGTTTAT
    CAATTACTAAATATAGATCGTGGTATTCCAGAAGTCATCAATAGTCCATTTGATCTTCGC
    GTCTTAATGGATGCCATATACGAACTGAATGACCACCAAGATTTGCGTGAGATTACTAAA
    GATTCGAAAATGCAAAAACTCGCATTAGCAGGATTCCTTAAAAAGATAAAAGGTACGTAC
    ATTGAGTCATTATTAAAAGAACACAAATTGTTATAA
    60
    120
    180
    240
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    360
    420
    480
    540
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    720
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    840
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    960
    1020
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    1140
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    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1776

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS00290 [old locus tag: NWMN_0050 ]
  • symbol: NWMN_RS00290
  • description: oleate hydratase
  • length: 591
  • theoretical pI: 4.75677
  • theoretical MW: 67648.2
  • GRAVY: -0.371235

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15.7)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 14.8)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) MCRA; MCRA family (PF06100; HMM-score: 746.4)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25)
    P-loop_NTPase (CL0023) AAA_15; AAA ATPase domain (PF13175; HMM-score: 15)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 39
    • Cmax: 0.122
    • Cmax_pos: 39
    • Smax: 0.232
    • Smax_pos: 42
    • Smean: 0.127
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446213987 NCBI
  • RefSeq: WP_000291842 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRDGQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAESIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDKFCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFTINRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKIEDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQKLALAGFLKKIKGTYIESLLKEHKLL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS00290 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]