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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02875 [old locus tag: NWMN_0492 ]
  • pan locus tag?: SAUPAN002298000
  • symbol: NWMN_RS02875
  • pan gene symbol?: cysS
  • synonym:
  • product: cysteine--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS02875 [old locus tag: NWMN_0492 ]
  • symbol: NWMN_RS02875
  • product: cysteine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 567611..569011
  • length: 1401
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (567611..569011) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGATTACATTATATAATACGCTTACACGTCAAAAAGAAGTGTTCAAGCCTATAGAACCA
    GGGAAAGTAAAAATGTATGTATGTGGTCCTACTGTATATAACTACATTCATATTGGTAAC
    GCAAGACCAGCAATTAATTATGACGTAGTGAGACGTTACTTTGAATACCAAGGATATAAT
    GTAGAATATGTATCAAATTTTACAGACGTAGATGATAAATTAATTAAACGTTCTCAAGAA
    TTAAATCAGTCTGTTCCCGAAATTGCAGAAAAATATATCGCTGCTTTTCATGAAGATGTT
    GGTGCGTTAAATGTTAGAAAAGCGACTTCAAATCCAAGGGTAATGGACCATATGGATGAC
    ATTATTCAATTTATTAAAGATTTGGTGGATCAAGGTTATGCATATGAAAGTGGTGGCGAT
    GTTTACTTTAGAACACGTAAATTTGAAGGTTATGGTAAATTAAGTCATCAATCCATAGAT
    GACTTAAAAGTGGGTGCTCGTATAGATGCAGGAGAGCATAAAGAAGATGCACTTGATTTT
    ACATTGTGGAAAAAAGCGAAGCCTGGCGAGATTAGTTGGGATAGCCCATTTGGTGAAGGT
    AGACCAGGATGGCATATAGAATGTTCTGTAATGGCATTTCATGAGCTAGGACCTACAATT
    GATATACATGCGGGTGGTTCAGATTTACAATTTCCACATCATGAAAATGAAATAGCACAA
    TCAGAAGCACATAATCATGCGCCATTTGCTAATTATTGGATGCATAATGGTTTCATTAAT
    ATTGATAATGAAAAAATGAGTAAATCACTAGGCAACTTTATTTTAGTTCACGATATTATT
    AAAGAAGTTGATCCAGATGTACTAAGATTCTTTATGATTAGCGTACATTATAGAAGCCCA
    ATTAACTATAATCTAGAATTGGTAGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAAT
    AGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACA
    TATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTT
    AATACAGCTAATGCAATTACAGCTTGGTATGATTTAGCAAAACTTGCGAATAAATATGTA
    CTAGAGAACACAACATCAACAGAAGTAATTGATAAATTTAAAGCAGTTTATCAAATTTTC
    AGCGATGTTTTAGGTGTACCGTTAAAATCTAAAAATGCAGATGAATTATTGGATGAAGAT
    GTTGAAAAATTAATCGAAGAGCGTAATGAAGCAAGGAAAAACAAAGATTTTGCACGAGCA
    GATGAAATTCGAGACATGCTGAAATCACAAAACATTATATTAGAAGACACACCTCAAGGG
    GTTAGATTTAAACGTGGATAA
    60
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    1140
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    1320
    1380
    1401

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS02875 [old locus tag: NWMN_0492 ]
  • symbol: NWMN_RS02875
  • description: cysteine--tRNA ligase
  • length: 466
  • theoretical pI: 5.03617
  • theoretical MW: 53684.9
  • GRAVY: -0.524678

Function[edit source | edit]

  • reaction:
    EC 6.1.1.16?  ExPASy
    Cysteine--tRNA ligaseATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 594.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogscysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 271)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 59.5)
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 46.1)
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 41.7)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 38.1)
    Genetic information processingProtein synthesistRNA aminoacylationlysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 28.2)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 27.2)
  • TheSEED:
  • PFAM:
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 441.7)
    DALR (CL0258) DALR_2; DALR domain (PF09190; HMM-score: 55.8)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 53.9)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 41.1)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 38)
    no clan definedPP2C_C; Protein serine/threonine phosphatase 2C, C-terminal domain (PF07830; HMM-score: 12.8)
    UVR; UvrB/uvrC motif (PF02151; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 18
    • Cmax: 0.135
    • Cmax_pos: 44
    • Smax: 0.308
    • Smax_pos: 10
    • Smean: 0.226
    • D: 0.186
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446554617 NCBI
  • RefSeq: WP_000631963 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPAINYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGALNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSIDDLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGPTIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILVHDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATNIENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDKFKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQNIILEDTPQGVRFKRG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS02875 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]