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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00511
  • pan locus tag?: SAUPAN002298000
  • symbol: cysS
  • pan gene symbol?: cysS
  • synonym:
  • product: cysteinyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00511
  • symbol: cysS
  • product: cysteinyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 513694..515094
  • length: 1401
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGATTACATTATATAATACGCTTACACGTCAAAAAGAAGTGTTCAAGCCTATAGAACCA
    GGGAAAGTAAAAATGTATGTATGTGGTCCTACTGTATATAACTACATTCATATTGGTAAC
    GCAAGACCAGCAATTAATTATGACGTAGTGAGACGTTACTTTGAATACCAAGGATATAAT
    GTAGAATATGTATCAAATTTTACAGACGTAGATGATAAATTAATTAAACGTTCTCAAGAA
    TTAAATCAGTCTGTTCCCGAAATTGCAGAAAAATATATCGCTGCTTTTCATGAAGATGTT
    GGTGCGTTAAATGTTAGAAAAGCGACTTCAAATCCAAGGGTAATGGACCATATGGATGAC
    ATTATTCAATTTATTAAAGATTTGGTGGATCAAGGTTATGCATATGAAAGTGGTGGCGAT
    GTTTACTTTAGAACACGTAAATTTGAAGGTTATGGTAAATTAAGTCATCAATCCATAGAT
    GACTTAAAAGTGGGTGCTCGTATAGATGCAGGAGAGCATAAAGAAGATGCACTTGATTTT
    ACATTGTGGAAAAAAGCGAAGCCTGGCGAGATTAGTTGGGATAGCCCATTTGGTGAAGGT
    AGACCAGGATGGCATATAGAATGTTCTGTAATGGCATTTCATGAGCTAGGACCTACAATT
    GATATACATGCGGGTGGTTCAGATTTACAATTTCCACATCATGAAAATGAAATAGCACAA
    TCAGAAGCACATAATCATGCGCCATTTGCTAATTATTGGATGCATAATGGTTTCATTAAT
    ATTGATAATGAAAAAATGAGTAAATCACTAGGCAACTTTATTTTAGTTCACGATATTATT
    AAAGAAGTTGATCCAGATGTACTAAGATTCTTTATGATTAGCGTACATTATAGAAGCCCA
    ATTAACTATAATCTAGAATTGGTAGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAAT
    AGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACA
    TATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTT
    AATACAGCTAATGCAATTACAGCTTGGTATGATTTAGCAAAACTTGCGAATAAATATGTA
    CTAGAGAACACAACATCAACAGAAGTAATTGATAAATTTAAAGCAGTTTATCAAATTTTC
    AGCGATGTTTTAGGTGTACCGTTAAAATCTAAAAATGCAGATGAATTATTGGATGAAGAT
    GTTGAAAAATTAATCGAAGAGCGTAATGAAGCAAGGAAAAACAAAGATTTTGCACGAGCA
    GATGAAATTCGAGACATGCTGAAATCACAAAACATTATATTAGAAGACACACCTCAAGGG
    GTTAGATTTAAACGTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00511
  • symbol: CysS
  • description: cysteinyl-tRNA synthetase
  • length: 466
  • theoretical pI: 5.03617
  • theoretical MW: 53684.9
  • GRAVY: -0.524678

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.16?  ExPASy
    Cysteine--tRNA ligase ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 594.1)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 271)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 59.5)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 46.1)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 41.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 38.1)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 28.2)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 27.2)
  • TheSEED  :
    • Cysteinyl-tRNA synthetase (EC 6.1.1.16)
    Miscellaneous Plant-Prokaryote comparative genomics Conserved gene cluster possibly involved in RNA metabolism  Cysteinyl-tRNA synthetase (EC 6.1.1.16)
  • PFAM:
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 441.7)
    and 6 more
    DALR (CL0258) DALR_2; DALR domain (PF09190; HMM-score: 55.8)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 53.9)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 41.1)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 38)
    no clan defined PP2C_C; Protein serine/threonine phosphatase 2C, C-terminal domain (PF07830; HMM-score: 12.8)
    UVR; UvrB/uvrC motif (PF02151; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.063455
    • TAT(Tat/SPI): 0.000583
    • LIPO(Sec/SPII): 0.004528
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPAINYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGALNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSIDDLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGPTIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILVHDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATNIENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDKFKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQNIILEDTPQGVRFKRG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Cys) (transcription antitermination) regulon
    T-box(Cys)(5' cis-acting region)important in Amino acid metabolism; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 4.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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