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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03040 [old locus tag: NWMN_0525 ]
  • pan locus tag?: SAUPAN002337000
  • symbol: NWMN_RS03040
  • pan gene symbol?: sdrE
  • synonym:
  • product: MSCRAMM family adhesin SdrE

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03040 [old locus tag: NWMN_0525 ]
  • symbol: NWMN_RS03040
  • product: MSCRAMM family adhesin SdrE
  • replicon: chromosome
  • strand: +
  • coordinates: 609219..612719
  • length: 3501
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (609219..612719) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    1021
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    ATGATTAACAGGGATAATAAAAAGGCAATAACAAAAAAGGGTATGATTTCAAATCGCTTA
    AACAAATTTTCGATTAGAAAGTATACTGTAGGAACTGCATCGATTTTAGTAGGTACGACA
    TTGATTTTTGGTCTAGGGAACCAAGAAGCTAAAGCTGCTGAAAACACTAGTACAGAAAAT
    GCAAAACAAGATGATGCAACGACTAGTGATAATAAAGAAGTAGTGTCGGAAACTGAAAAT
    AATTCGACAACAGAAAATAATTCAACAAATCCAATTAAGAAAGAAACAAATACTGATTCA
    CAACCAGAAGCTAAAAAAGAATCAACTTCATCAAGTACTCAAAAACAGCAAAATAACGTT
    ACAGCTACAACTGAAACTAAGCCTCAAAACATTGAAAAAGAAAATGTTAAACCTTCAACT
    GATAAAACTGCGACAGAAGATACATCTGTTATTTTAGAAGAGAAGAAAGCACCAAATAAT
    ACAAATAACGATGTAACTACAAAACCATCTACAAGTGAACCATCTACAAGTGAAATTCAA
    ACAAAACCAACTACACCTCAAGAATCTACAAATATTGAAAATTCACAACCGCAACCAACG
    CCTTCAAAAGTAGACAATCAAGTTACAGATGCAACTAATCCAAAAGAACCAGTAAATGTG
    TCAAAAGAAGAACTTAAAAATAATCCTGAGAAATTAAAAGAATTGGTTAGAAATGATAGC
    AATACAGATCATTCAACTAAACCAGTTGCTACAGCTCCAACAAGTGTTGCACCAAAACGT
    GTAAACGCAAAAATGCGCTTTGCAGTTGCACAACCAGCAGCAGTTGCTTCAAACAATGTA
    AATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTG
    GCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAA
    GTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTA
    ACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGA
    ACATTTGATAAAGCAACTAAGCAAATCACATATACATTTACAGACTATGTAGATAAATAT
    GAAGATATAAAATCACGCTTAACTCTATATTCGTATATTGATAAAAAAACAGTTCCAAAT
    GAGACAAGTTTGAATTTAACATTTGCTACAGCAGGTAAAGAAACAAGCCAAAATGTCACT
    GTTGATTATCAAGATCCAATGGTCCATGGTGATTCAAACATTCAATCTATCTTTACAAAA
    TTAGATGAAGATAAGCAAACTATTGAACAACAAATTTATGTTAACCCATTGAAAAAATCA
    GCAACCAACACTAAAGTTGATATAGCTGGTAGTCAAGTAGATGATTATGGAAATATTAAA
    CTAGGAAATGGTAGCACCATTATTGACCAAAATACAGAAATAAAGGTTTATAAAGTTAAC
    TCTGATCAACAATTGCCTCAAAGTAATAGAATCTATGATTTTAGTCAATACGAAGATGTA
    ACAAGTCAATTTGATAATAAAAAATCATTTAGTAATAATGTAGCAACATTGGATTTTGGT
    GATATTAATTCAGCCTATATTATCAAAGTTGTTAGTAAATATACACCTACATCAGATGGC
    GAACTAGATATTGCCCAAGGTACTAGTATGAGAACAACTGATAAATATGGTTATTATAAT
    TATGCAGGATATTCAAACTTCATCGTAACTTCTAATGACACTGGCGGTGGCGACGGTACT
    GTTAAACCTGAAGAAAAGTTATACAAAATTGGTGACTATGTATGGGAAGACGTTGATAAA
    GACGGTGTTCAAGGTACAGATTCAAAAGAAAAACCAATGGCAAACGTTTTAGTTACATTA
    ACTTACCCGGACGGTACTACAAAATCAGTAAGAACAGATGCTAATGGTCATTATGAATTC
    GGTGGTTTGAAAGACGGAGAAACTTATACAGTTAAATTCGAAACGCCAACTGGATATCTT
    CCAACAAAAGTAAATGGAACAACTGATGGTGAAAAAGACTCAAATGGTAGTTCGGTTACT
    GTTAAAATTAATGGTAAAGATGATATGTCTTTAGATACTGGTTTTTACAAAGAACCTAAA
    TACAACTTAGGTGACTATGTATGGGAAGATACTAATAAAGATGGTATCCAAGATGCAAAT
    GAGCCAGGAATCAAAGATGTTAAGGTTACATTAAAAGATAGTACTGGAAAAGTTATTGGT
    ACAACTACTACTGATGCCTCGGGTAAATATAAATTTACAGATTTAGATAATGGTAACTAT
    ACAGTAGAATTTGAAACACCAGCAGGTTACACGCCAACGGTTAAAAATACTACAGCTGAT
    GATAAAGATTCTAATGGTTTAACAACAACAGGTGTCATTAAAGATGCAGATAATATGACA
    TTAGACAGGGGTTTCTATAAAACACCAAAATACAGTTTAGGTGATTATGTTTGGTACGAC
    AGTAATAAAGACGGCAAACAAGATTCAACTGAAAAAGGTATCAAAGATGTGACAGTTACA
    TTGCAAAACGAAAAAGGCGAAGTAATTGGAACAACTAAAACAGATGAAAATGGTAAATAT
    CGTTTCGATAATTTAGATAGCGGTAAATACAAAGTTATTTTTGAAAAGCCTGCTGGCTTA
    ACACAAACAGTTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGAC
    GTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACA
    TCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGAC
    TCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAT
    TCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAC
    AGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGAC
    TCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAT
    TCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAT
    AGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGAC
    TCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAC
    AGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAA
    GACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAAT
    AACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGT
    CGCAAAAAACAAAACAAATAA
    60
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    3501

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS03040 [old locus tag: NWMN_0525 ]
  • symbol: NWMN_RS03040
  • description: MSCRAMM family adhesin SdrE
  • length: 1166
  • theoretical pI: 4.02054
  • theoretical MW: 126548
  • GRAVY: -1.10214

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 50.1)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 16.2)
    Por secretion system C-terminal sorting domain (TIGR04183; HMM-score: 14.4)
  • TheSEED:
  • PFAM:
    Transthyretin (CL0287) SdrD_B; SdrD B-like domain (PF17210; HMM-score: 251.6)
    CarboxypepD_reg; Carboxypeptidase regulatory-like domain (PF13620; HMM-score: 105.7)
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 104.1)
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 45.4)
    E-set (CL0159) MG1; Alpha-2-macroglobulin MG1 domain (PF11974; HMM-score: 44.9)
    no clan definedGram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 42.6)
    Transthyretin (CL0287) MetallophosN; N terminal of Calcineurin-like phosphoesterase (PF16371; HMM-score: 41.2)
    CarbopepD_reg_2; CarboxypepD_reg-like domain (PF13715; HMM-score: 30)
    DUF2012; Protein of unknown function (DUF2012) (PF09430; HMM-score: 22.9)
    DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 17.8)
    no clan definedCompInhib_SCIN; Staphylococcal complement inhibitor SCIN (PF11546; HMM-score: 16.4)
    GOLD-like (CL0521) EMP24_GP25L; emp24/gp25L/p24 family/GOLD (PF01105; HMM-score: 14.1)
    Transthyretin (CL0287) DUF1416; Protein of unknown function (DUF1416) (PF07210; HMM-score: 14)
    Dioxygenase_C; Dioxygenase (PF00775; HMM-score: 13.2)
    OB (CL0021) DUF4539; Domain of unknown function (DUF4539) (PF15072; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide length 53 aa
    • Ymax: 0.731
    • Ymax_pos: 53
    • Cmax: 0.791
    • Cmax_pos: 53
    • Smax: 0.857
    • Smax_pos: 49
    • Smean: 0.453
    • D: 0.623
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

  • GI: 446532960 NCBI
  • RefSeq: WP_000610306 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQPEAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNTNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPTKVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTADDKDSNGLTTTGVIKDADNMTLDRGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS03040 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]