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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • pan locus tag?: SAUPAN002473000
  • symbol: NWMN_RS03315
  • pan gene symbol?: argS
  • synonym:
  • product: arginine--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • symbol: NWMN_RS03315
  • product: arginine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 659531..661192
  • length: 1662
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (659531..661192) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    61
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    ATGAATATTATTGATCAAGTGAAACAAACATTAGTAGAAGAAATTGCAGCAAGTATTAAC
    AAAGCAGGATTAGCAGATGAGATTCCTGATATTAAAATTGAAGTTCCTAAAGATACAAAA
    AATGGAGATTATGCTACTAATATTGCGATGGTACTGACTAAGATTGCAAAGCGTAATCCT
    CGTGAAATTGCTCAAGCGATTGTTGATAACTTAGATACTGAAAAAGCACATGTAAAACAA
    ATTGACATTGCTGGTCCAGGATTCATTAATTTTTACTTAGATAATCAGTATTTAACAGCA
    ATTATTCCTGAAGCAATTGAAAAAGGTGATCAATTTGGACATGTAAATGAATCAAAAGGT
    CAAAATGTATTGCTTGAGTATGTTTCAGCTAACCCTACAGGAGATTTACATATTGGTCAT
    GCTAGAAATGCAGCAGTTGGTGATGCTTTAGCTAATATTTTAACTGCAGCTGGCTATAAT
    GTAACACGTGAATATTATATTAATGATGCTGGTAATCAAATTACTAACTTAGCGCGTTCG
    ATTGAAACACGTTTCTTTGAAGCTTTAGGTGACAATAGTTATTCAATGCCAGAAGATGGC
    TATAATGGAAAAGATATTATTGAAATAGGTAAAGATTTAGCAGAGAAACACCCTGAAATT
    AAAGATTATTCTGAAGAAGCACGTTTGAAAGAATTTAGAAAATTAGGCGTAGAATACGAA
    ATGGCTAAATTGAAAAATGATTTAGCAGAGTTCAATACGCATTTTGATAATTGGTTTAGT
    GAAACATCTTTATATGAAAAAGGAGAAATTCTTGAAGTTTTAGCAAAAATGAAAGAATTA
    GGTTATACGTATGAAGCTGATGGCGCTACATGGTTACGTACAACTGATTTTAAAGACGAC
    AAAGACAGAGTATTAATTAAAAATGACGGTACATATACGTATTTCTTACCAGATATTGCG
    TACCACTTCGATAAAGTAAAACGTGGTAATGACATTTTAATCGATTTATTTGGTGCTGAT
    CATCATGGTTATATTAATCGTTTGAAAGCATCTCTTGAAACGTTTGGTGTAGATAGTAAT
    CGTTTAGAAATTCAAATCATGCAAATGGTTCGTTTAATGGAAAATGGTAAAGAAGTGAAG
    ATGAGTAAACGTACTGGTAATGCGATTACATTAAGAGAAATTATGGACGAAGTTGGCGTT
    GACGCTGCACGTTATTTCTTAACTATGCGTAGTCCTGATAGTCACTTTGATTTTGATATG
    GAATTAGCGAAAGAGCAATCTCAAGACAATCCAGTTTACTATGCTCAATATGCACATGCG
    CGTATTTGTTCAATTTTAAAACAAGCGAAAGAGCAAGGTATTGAAGTGACTGCTGCGAAT
    GATTTTACAACGATTACTAATGAAAAAGCGATTGAATTGTTGAAAAAAGTAGCTGATTTC
    GAACCTACAATTGAAAGTGCTGCTGAGCATAGATCGGCACATAGAATTACTAATTATATT
    CAAGATTTAGCTTCTCATTTCCATAAATTCTATAATGCTGAAAAAGTGTTAACAGATGAT
    ATTGAAAAAACAAAAGCACATGTTGCTATGATTGAAGCGGTCAGAATTACATTGAAAAAT
    GCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAA
    60
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    1662

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • symbol: NWMN_RS03315
  • description: arginine--tRNA ligase
  • length: 553
  • theoretical pI: 4.86205
  • theoretical MW: 62380.9
  • GRAVY: -0.425316

Function[edit source | edit]

  • reaction:
    EC 6.1.1.19?  ExPASy
    Arginine--tRNA ligaseATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationarginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 511.7)
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 42.6)
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 41.6)
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 37.1)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 33.7)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 31.4)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 22.7)
    Genetic information processingProtein synthesistRNA aminoacylationlysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 20.7)
  • TheSEED:
  • PFAM:
    DALR (CL0258) DALR_1; DALR anticodon binding domain (PF05746; HMM-score: 123.1)
    no clan definedArg_tRNA_synt_N; Arginyl tRNA synthetase N terminal domain (PF03485; HMM-score: 90.2)
    HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 89.8)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 38.3)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 33.9)
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 31.1)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 28)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 16.2)
    tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 15.7)
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_RS07455dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.147
    • Ymax_pos: 67
    • Cmax: 0.226
    • Cmax_pos: 67
    • Smax: 0.154
    • Smax_pos: 8
    • Smean: 0.092
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446943889 NCBI
  • RefSeq: WP_001021145 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDYATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAIIPEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYNVTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHPEIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAKMKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILIDLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLREIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAKEQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFHKFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS03315 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

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