NCBI: 02-MAR-2017
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS03945 [old locus tag: NWMN_0697 ]
- pan locus tag?: SAUPAN002626000
- symbol: NWMN_RS03945
- pan gene symbol?: queF
- synonym:
- product: NADPH-dependent 7-cyano-7-deazaguanine reductase QueF
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: NWMN_RS03945 [old locus tag: NWMN_0697 ]
- symbol: NWMN_RS03945
- product: NADPH-dependent 7-cyano-7-deazaguanine reductase QueF
- replicon: chromosome
- strand: -
- coordinates: 782076..782576
- length: 501
- essential: unknown
⊟Accession numbers[edit source | edit]
- Location: NC_009641 (782076..782576) NCBI
- MicrobesOnline:
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
- 1
61
121
181
241
301
361
421
481ATGGCACATGGCCGTCAACAAGATGAATTACAAGATATAACTTTATTAGGAAATCAAGAC
AATACTTACAATTTTGACTATCGTCCTGATGTATTAGAATCGTTTGATAATAAGCATCAA
GGTCGTGACTATTTCGTAAAATTCAACTGTCCAGAATTTACATCATTATGTCCAATTACT
GGGCAACCAGATTTTGCAACTATCTATATTTCATATATTCCAAATGTTAAAATGGTTGAA
TCAAAATCTTTGAAATTATACTTATTTAGTTTCAGAAATCACGGTGATTTTCACGAAGAT
TGTATGAATATTATTATGAATGATTTGATAGAGCTTATGGACCCACATTATATTGAAGTC
TGGGGCAAGTTCACACCACGTGGTGGAATTTCTATTGATCCTTATACAAACTATGGACGT
CCAAATTCTAAATATGAAAAAATGGCTGAGCATCGTTTGATGAATCATGATTTATATCCC
GAAAAAATAGATAATCGTTAA60
120
180
240
300
360
420
480
501
⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: NWMN_RS03945 [old locus tag: NWMN_0697 ]
- symbol: NWMN_RS03945
- description: NADPH-dependent 7-cyano-7-deazaguanine reductase QueF
- length: 166
- theoretical pI: 5.23642
- theoretical MW: 19645
- GRAVY: -0.715663
⊟Function[edit source | edit]
- reaction: EC 1.7.1.13? ExPASyPreQ1 synthase7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH
- TIGRFAM: 7-cyano-7-deazaguanine reductase (TIGR03139; EC 1.7.1.13; HMM-score: 177.6)TranscriptionRNA processingqueuine synthase (TIGR03138; EC 1.7.1.13; HMM-score: 82.1)
- TheSEED:
- PFAM: THBO-biosyn (CL0334) QueF; QueF-like protein (PF14489; HMM-score: 120.4)QueF_N; Nitrile reductase, 7-cyano-7-deazaguanine-reductase N-term (PF14819; HMM-score: 23.1)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- Ymax: 0.116
- Ymax_pos: 67
- Cmax: 0.14
- Cmax_pos: 67
- Smax: 0.125
- Smax_pos: 39
- Smean: 0.084
- D: 0.103
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDYFVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYPEKIDNR
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- NWMN_RS03945 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]