From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04195 [old locus tag: NWMN_0741 ]
  • pan locus tag?: SAUPAN002706000
  • symbol: NWMN_RS04195
  • pan gene symbol?: gapA
  • synonym:
  • product: aldehyde dehydrogenase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS04195 [old locus tag: NWMN_0741 ]
  • symbol: NWMN_RS04195
  • product: aldehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 833956..834966
  • length: 1011
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (833956..834966) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0741

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCAGTAAAAGTAGCAATTAATGGTTTTGGTAGAATTGGTCGTTTAGCATTCAGAAGA
    ATTCAAGAAGTAGAAGGTCTTGAAGTTGTAGCAGTAAACGACTTAACAGATGACGACATG
    TTAGCGCATTTATTAAAATATGACACTATGCAAGGTCGTTTCACAGGTGAAGTAGAGGTA
    GTTGATGGTGGTTTCCGCGTAAATGGTAAAGAAGTTAAATCATTCAGTGAACCAGATGCA
    AGCAAATTACCTTGGAAAGACTTAAATATCGATGTAGTATTAGAATGTACTGGTTTCTAC
    ACTGATAAAGATAAAGCACAAGCTCATATTGAAGCAGGCGCTAAAAAAGTATTAATCTCA
    GCACCAGCTACTGGTGACTTAAAAACAATCGTATTCAACACTAACCACCAAGAGTTAGAC
    GGTTCTGAAACAGTTGTTTCAGGTGCTTCATGTACTACAAACTCATTAGCACCAGTTGCT
    AAAGTTTTAAACGATGACTTTGGTTTAGTTGAAGGTTTAATGACTACAATTCACGCTTAC
    ACAGGTGATCAAAATACACAAGACGCACCTCACAGAAAAGGTGACAAACGTCGTGCTCGT
    GCAGCGGCAGAAAACATCATCCCTAACTCAACAGGTGCTGCTAAAGCTATCGGTAAAGTT
    ATTCCTGAAATCGATGGTAAATTAGATGGTGGTGCACAACGTGTTCCTGTAGCTACAGGT
    TCATTAACTGAATTAACAGTAGTATTAGAAAAACAAGACGTAACAGTTGAACAAGTTAAC
    GAAGCTATGAAAAATGCTTCAAACGAATCATTCGGTTACACTGAAGACGAAATCGTTTCT
    TCAGACGTTGTAGGTATGACTTACGGTTCATTATTCGACGCTACACAAACTCGTGTAATG
    TCAGTTGGCGACCGTCAATTAGTTAAAGTTGCAGCTTGGTATGATAACGAAATGTCATAT
    ACTGCACAATTAGTTCGTACATTAGCATACTTAGCTGAACTTTCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS04195 [old locus tag: NWMN_0741 ]
  • symbol: NWMN_RS04195
  • description: aldehyde dehydrogenase
  • length: 336
  • theoretical pI: 4.64965
  • theoretical MW: 36280.6
  • GRAVY: -0.218155

Function[edit | edit source]

  • reaction:
    EC 1.2.1.-?  ExPASy
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 435.2)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 283.8)
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 14.4)
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 13.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 204)
    and 4 more
    NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 124.2)
    DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 18.2)
    GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 15.7)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002695
    • TAT(Tat/SPI): 0.000131
    • LIPO(Sec/SPII): 0.000272
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVAKVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIGKVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTEDEIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAELSK

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]