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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04690 [old locus tag: NWMN_0831 ]
  • pan locus tag?: SAUPAN003067000
  • symbol: NWMN_RS04690
  • pan gene symbol?: argH
  • synonym:
  • product: argininosuccinate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS04690 [old locus tag: NWMN_0831 ]
  • symbol: NWMN_RS04690
  • product: argininosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 918719..920098
  • length: 1380
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (918719..920098) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAATAAAGCTTGGGGCGGTAGATTTGAAGTACAACCTGAAGAGTGGGTTGACGAC
    TTTAACGCATCCATTACTTTTGATCAAACGCTCATAGATCAAGATATCGAAGGCAGCATT
    GCACATGCAACTATGCTTGCGAATCAAGGCATTATTAGTCAACAAGACAGCGAACAAATT
    ATACAAGGACTAAAATCTATTCAACATGATTATCATCAAGATCAAATTCAATTTAGTGCA
    TCATTAGAAGATATTCATTTAAATATTGAACATGAATTAATTAAACGTATCGGTGATGCT
    GGTGGTAAGTTGCATACTGGACGCAGTAGAAACGATCAAGTTGCAACAGACATGCACTTG
    TACACTAAGAAACAAGTGCAAGATATCATCGCATTAATTAAGTCGTTACAAAGTGTAATT
    GTAGACATCGCTTCCAATAATGTTGATACAATTATGCCTGGTTATACTCATTTACAGCGT
    GCACAGCCAATTTCATTTGCACATCATATTATGACTTATTTTTGGATGTTACAACGAGAC
    CAACAACGATTTGAAGATAGTTTAAAACGAATCGATATTAATCCTTTAGGTGCAGCAGCC
    TTAAGTGGTACCACATACCCTATCGATAGACACGAGACAACAGCATTGTTGAACTTTGGC
    AGTCTCTATGAGAATAGCCTAGATGCTGTTAGTGACAGAGACTATATTATTGAAACATTG
    CATAATATTTCTTTAACGATGGTTCACTTATCACGCTTTGCAGAGGAAATTATTTTCTGG
    TCCACAGACGAAGCTAAATTCATTACATTATCAGATGCATTTTCAACTGGCTCATCTATT
    ATGCCACAAAAGAAAAATCCTGATATGGCAGAATTAATTAGAGGTAAAGTTGGTCGAACG
    ACTGGTCATTTAATGAGCATGCTTATGACTTTAAAAGGATTACCTCTAGCATATAACAAA
    GATATGCAGGAAGATAAAGAAGGTTTATTCGATGCTGTCCATACAATTAAAGGTTCTTTA
    CGTATTTTCGAAGGTATGATTCAAACGATGACAATTAATAAAGAACGACTCAATCAAACT
    GTTAAAGAAGATTTTTCAAATGCAACGGAACTAGCAGATTATTTAGTAACTAAAAATATT
    CCATTTAGAACTGCACATGAAATTGTAGGAAAAATCGTCTTAGAATGTATACAACAAGGT
    CATTATTTATTAGATGTTCCTTTAGCAACATATCAACAACATCATTCTAGTATTGATGCC
    GATATTTACGATTATTTGCAGCCTGAAAATTGTTTAAAACGACGTCAAAGTTACGGTTCA
    ACAGGTCAATCATCGGTCAAACAACAACTTGATGTTGCTAAACAATTACTATCACAATAA
    60
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    1020
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    1320
    1380

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS04690 [old locus tag: NWMN_0831 ]
  • symbol: NWMN_RS04690
  • description: argininosuccinate lyase
  • length: 459
  • theoretical pI: 5.51254
  • theoretical MW: 52016.5
  • GRAVY: -0.361438

Function[edit source | edit]

  • reaction:
    EC 4.3.2.1?  ExPASy
    Argininosuccinate lyase2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 659.5)
    MetabolismEnergy metabolismOther3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 80.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 76)
    MetabolismEnergy metabolismAmino acids and aminesaspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 63.2)
    MetabolismEnergy metabolismTCA cyclefumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 48)
  • TheSEED:
  • PFAM:
    no clan definedLyase_1; Lyase (PF00206; HMM-score: 252.1)
    ASL_C2; Argininosuccinate lyase C-terminal (PF14698; HMM-score: 81.7)
    Hpt; Hpt domain (PF01627; HMM-score: 16.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 56
    • Cmax: 0.126
    • Cmax_pos: 56
    • Smax: 0.157
    • Smax_pos: 53
    • Smean: 0.065
    • D: 0.091
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445988198 NCBI
  • RefSeq: WP_000066053 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHATMLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGGKLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQRAQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLNFGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFSTGSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVHTIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKIVLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQLDVAKQLLSQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS04690 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]