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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0963 [new locus tag: SACOL_RS04940 ]
  • pan locus tag?: SAUPAN003067000
  • symbol: argH
  • pan gene symbol?: argH
  • synonym:
  • product: argininosuccinate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0963 [new locus tag: SACOL_RS04940 ]
  • symbol: argH
  • product: argininosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 965546..966925
  • length: 1380
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAATAAAGCTTGGGGCGGTAGATTTGAAGTACAACCTGAAGAGTGGGTTGACGAC
    TTTAACGCATCCATTACTTTTGATCAAACGCTCATAGATCAAGATATCGAAGGCAGCATT
    GCACATGCAACTATGCTTGCGAATCAAGGCATTATTAGTCAACAAGACAGCGAACAAATT
    ATACAAGGACTAAAATCTATTCAACATGATTATCATCAAGATCAAATTCAATTTAGTGCA
    TCATTAGAAGATATTCATTTAAATATTGAACATGAATTAATTAAACGTATCGGTGATGCT
    GGTGGTAAGTTGCATACTGGACGCAGTAGAAACGATCAAGTTGCAACAGACATGCACTTG
    TACACTAAGAAACAAGTGCAAGATATCATCGCATTAATTAAGTCGTTACAAAGTGTAATT
    GTAGACATCGCTTCCAATAATGTTGATACAATTATGCCTGGTTATACTCATTTACAGCGT
    GCACAGCCAATTTCATTTGCACATCATATTATGACTTATTTTTGGATGTTACAACGAGAC
    CAACAACGATTTGAAGATAGTTTAAAACGAATCGATATTAATCCTTTAGGTGCAGCAGCC
    TTAAGTGGTACCACATACCCTATCGATAGACACGAGACAACAGCATTGTTGAACTTTGGC
    AGTCTCTATGAGAATAGCCTAGATGCTGTTAGTGACAGAGACTATATTATTGAAACATTG
    CATAATATTTCTTTAACGATGGTTCACTTATCACGCTTTGCAGAGGAAATTATTTTCTGG
    TCCACAGACGAAGCTAAATTCATTACATTATCAGATGCATTTTCAACTGGCTCATCTATT
    ATGCCACAAAAGAAAAATCCTGATATGGCAGAATTAATTAGAGGTAAAGTTGGTCGAACG
    ACTGGTCATTTAATGAGCATGCTTATGACTTTAAAAGGATTACCTCTAGCATATAACAAA
    GATATGCAGGAAGATAAAGAAGGTTTATTCGATGCTGTCCATACAATTAAAGGTTCTTTA
    CGTATTTTCGAAGGTATGATTCAAACGATGACAATTAATAAAGAACGACTCAATCAAACT
    GTTAAAGAAGATTTTTCAAATGCAACGGAACTAGCAGATTATTTAGTAACTAAAAATATT
    CCATTTAGAACTGCACATGAAATTGTAGGAAAAATCGTCTTAGAATGTATACAACAAGGT
    CATTATTTATTAGATGTTCCTTTAGCAACATATCAACAACATCATTCTAGTATTGATGCC
    GATATTTACGATTATTTGCAGCCTGAAAATTGTTTAAAACGACGTCAAAGTTACGGTTCA
    ACAGGTCAATCATCGGTCAAACAACAACTTGATGTTGCTAAACAATTACTATCACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0963 [new locus tag: SACOL_RS04940 ]
  • symbol: ArgH
  • description: argininosuccinate lyase
  • length: 459
  • theoretical pI: 5.51254
  • theoretical MW: 52016.5
  • GRAVY: -0.361438

Function[edit source | edit]

  • reaction:
    EC 4.3.2.1?  ExPASy
    Argininosuccinate lyase2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 659.5)
    MetabolismEnergy metabolismOther3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 80.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 76)
    MetabolismEnergy metabolismAmino acids and aminesaspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 63.2)
    MetabolismEnergy metabolismTCA cyclefumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 48)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine Biosynthesis extended Argininosuccinate lyase (EC 4.3.2.1) 
  • PFAM:
    no clan definedLyase_1; Lyase (PF00206; HMM-score: 252.1)
    ASL_C2; Argininosuccinate lyase C-terminal (PF14698; HMM-score: 81.7)
    Hpt; Hpt domain (PF01627; HMM-score: 16.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 56
    • Cmax: 0.126
    • Cmax_pos: 56
    • Smax: 0.157
    • Smax_pos: 53
    • Smean: 0.065
    • D: 0.091
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHATMLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGGKLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQRAQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLNFGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFSTGSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVHTIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKIVLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQLDVAKQLLSQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: ArgR, CodY
    ArgR (TF) important in Arginine biosynthesis, Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    CodY (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]