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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05250 [old locus tag: NWMN_0937 ]
  • pan locus tag?: SAUPAN003282000
  • symbol: NWMN_RS05250
  • pan gene symbol?: purL
  • synonym:
  • product: phosphoribosylformylglycinamidine synthase subunit PurL

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS05250 [old locus tag: NWMN_0937 ]
  • symbol: NWMN_RS05250
  • product: phosphoribosylformylglycinamidine synthase subunit PurL
  • replicon: chromosome
  • strand: +
  • coordinates: 1039325..1041514
  • length: 2190
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1039325..1041514) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGTCTAAATTTATCGAACCAAGCGTTGAAGAAATTAAACTTGAAAAAGTATATCAAGAT
    ATGGGATTAAGTGATCAAGAATATGAAAAAGTTTGCGATATTTTAGGCAGACAACCTAAC
    TTTACAGAAACAGGTATCTTTTCTGTTATGTGGAGTGAACATTGCTCTTATAAACATTCT
    AAACCGTTTTTAAAGCAATTTCCTACGTCAGGTGACCATGTGCTTATGGGGCCTGGTGAA
    GGTGCAGGGGTAGTCGATATAGGTGATAATCAAGCCGTAGTATTTAAAGTAGAGTCTCAC
    AATCATCCATCAGCAATTGAACCATATCAAGGGGCTGCTACAGGCGTTGGTGGAATCATT
    CGTGACATTGTCTCTATTGGGGCTAGACCTATTAATTTGTTAAACAGTCTTAGATTTGGA
    GAATTAGATAATAAACAAAACCAAAGATTACTTAAAGGTGTTGTAAAGGGTATCGGAGGT
    TATGGTAACTGCATTGGTATTCCAACAACTGCTGGTGAAATCGAATTTGATGAACGTTAT
    GATGGCAATCCACTTGTTAATGCAATGTGTGTTGGTGTTATCAATCACGACATGATTCAA
    AAAGGCACAGCAAAAGGTGTAGGTAATTCGGTCATTTATGTTGGTTTGAAAACTGGTCGA
    GATGGTATTCATGGTGCTACTTTTGCATCTGAAGAATTGACGGAAGAAAGCGAAAGTAAA
    CGACCTTCTGTACAAATCGGTGATCCATTTGTAGGTAAAAAATTAATGGAAGCAACACTT
    GAAGCAATTACATTTGATGAATTAGTTGGTATTCAAGATATGGGTGCTGCTGGTTTAACA
    TCTTCATCGTCTGAAATGGCGGCAAAAGGTGGTAGTGGGTTACATTTGAGATTAGAACAA
    GTGCCAACACGTGAGCCAGGTATTTCTCCTTATGAAATGATGCTTTCAGAAACTCAAGAA
    CGTATGTTACTAGTTGTTGAAAAAGGTACTGAACAAAAATTCTTAGATTTATTTGATAAG
    CACGAATTGGATAGTGCTGTTATAGGTGAAGTTACAGATACAAATCGTTTTGTTTTAACA
    TATGATGACGAAGTTTATGCTGACATTCCAGTTGAACCACTAGCTGATGAAGCACCTGTA
    TATATTTTAGAAGGAGAAGAAAAAGATTATAATACTTCTAAAAATGATTATACACACATC
    GATGTTAAAGATACTTTCTTTAAATTACTTAAGCATCCGACTATAGCATCTAAACACTAT
    TTATATGATCAATACGACCAACAAGTTGGTGCCAATACGATAATTAAGCCAGGACTTCAA
    GCATCGGTAGTACGTGTGGAAGGCACAAATAAGGCAATTGCTTCAACAATTGATGGTGAA
    GCGCGTTATGTATATAACAATCCATATGAAGGTGGAAAGATGGTAGTAGCTGAAGCTTAT
    CGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGT
    TCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAA
    GCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATAACGAAACG
    AAAGGTACTTCTATTTTCCCAACACCAGTTGTTGGAATGGTAGGTTTGATTGAAAATGTA
    AATTATTTAAATGATTTTGAACCTCAAGTTGGAGATAAATTATATTTAATCGGTGATACT
    AAGGACGACTTTGGTGGTAGTCAACTTGAAAAGTTAATTTATGGCAAAGTTAATCATGAA
    TTTGAGTCATTAGATTTGAGTTCAGAAGTTGAAAAAGGTGAATCAATCAAGACCGCTATT
    CGTGAAGGACTATTATCACATGTTCAAACAGTTGGTAAAGGTGGCTTACTGATTACCTTA
    GCTAAACTAAGTGCGCATTACGGTTTAGGATTAAAATCTTCAATAGATATAACAAATGCA
    CAATTGTTTAGTGAGACGCAAGGCCGATATGTTGTTTCTGTTAAATCAGGTAAAACTTTA
    AATATTGATAATGCAATAGAAATTGGACTTTTAACAGATAGTGATAATTTCAAGGTAACA
    ACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATATGGGAAGGGGCA
    ATTGCTCAATGTTTAACTACTCAGGATTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS05250 [old locus tag: NWMN_0937 ]
  • symbol: NWMN_RS05250
  • description: phosphoribosylformylglycinamidine synthase subunit PurL
  • length: 729
  • theoretical pI: 4.50802
  • theoretical MW: 79535.2
  • GRAVY: -0.253086

Function[edit source | edit]

  • reaction:
    EC 6.3.5.3?  ExPASy
    Phosphoribosylformylglycinamidine synthaseATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 1004.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 166.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 139)
    MetabolismEnergy metabolismMethanogenesisputative methanogenesis marker protein 2 (TIGR03267; HMM-score: 64.6)
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 37.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 37.1)
    Unknown functionEnzymes of unknown specificityAIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 35.9)
    herpesvirus tegument protein/v-FGAM-synthase (TIGR01739; HMM-score: 12.6)
  • TheSEED:
  • PFAM:
    no clan definedAIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 157.8)
    AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 131.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 30
    • Cmax: 0.111
    • Cmax_pos: 61
    • Smax: 0.137
    • Smax_pos: 19
    • Smean: 0.086
    • D: 0.095
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445954872 NCBI
  • RefSeq: WP_000032727 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTETGIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNHPSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGGYGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKTGRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGAAGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKFLDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNTSKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTNKAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIYQQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDFEPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGLLSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNIDNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS05250 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]