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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06145 [old locus tag: NWMN_1084 ]
  • pan locus tag?: SAUPAN003440000
  • symbol: NWMN_RS06145
  • pan gene symbol?: psmβ1
  • synonym: psmB1
  • product: hemolytic protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS06145 [old locus tag: NWMN_1084 ]
  • symbol: NWMN_RS06145
  • product: hemolytic protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1188408..1188542
  • length: 135
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1188408..1188542) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1084

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    ATGGAAGGTTTATTTAACGCAATTAAAGATACCGTAACTGCAGCAATTAATAATGATGGC
    GCAAAATTAGGCACAAGCATTGTGAGCATCGTTGAAAATGGCGTAGGTTTATTAGGTAAA
    TTATTCGGATTCTAA
    60
    120
    135

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS06145 [old locus tag: NWMN_1084 ]
  • symbol: NWMN_RS06145
  • description: hemolytic protein
  • length: 44
  • theoretical pI: 4.54462
  • theoretical MW: 4496.17
  • GRAVY: 0.570455

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Staph_haemo; Staphylococcus haemolytic protein (PF05480; HMM-score: 83.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.150184
    • TAT(Tat/SPI): 0.002632
    • LIPO(Sec/SPII): 0.009262
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Rong Wang, Kevin R Braughton, Dorothee Kretschmer, Thanh-Huy L Bach, Shu Y Queck, Min Li, Adam D Kennedy, David W Dorward, Seymour J Klebanoff, Andreas Peschel, Frank R DeLeo, Michael Otto
Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA.
Nat Med: 2007, 13(12);1510-4
[PubMed:17994102] [WorldCat.org] [DOI] (I p)
Shu Y Queck, Max Jameson-Lee, Amer E Villaruz, Thanh-Huy L Bach, Burhan A Khan, Daniel E Sturdevant, Stacey M Ricklefs, Min Li, Michael Otto
RNAIII-independent target gene control by the agr quorum-sensing system: insight into the evolution of virulence regulation in Staphylococcus aureus.
Mol Cell: 2008, 32(1);150-8
[PubMed:18851841] [WorldCat.org] [DOI] (I p)
Michael Otto
Staphylococcus aureus toxins.
Curr Opin Microbiol: 2014, 17;32-7
[PubMed:24581690] [WorldCat.org] [DOI] (I p)