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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07350 [old locus tag: NWMN_1305 ]
  • pan locus tag?: SAUPAN003807000
  • symbol: NWMN_RS07350
  • pan gene symbol?: asd
  • synonym:
  • product: aspartate-semialdehyde dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS07350 [old locus tag: NWMN_1305 ]
  • symbol: NWMN_RS07350
  • product: aspartate-semialdehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1438052..1439041
  • length: 990
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1438052..1439041) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    961
    ATGACAAAGTTAGCAGTTGTGGGTGCAACAGGATTAGTAGGTACAAAAATGTTGGAGACA
    TTAAATCGTAAAAATATTCCTTTCGATGAATTAGTATTATTTTCATCAGCACGTTCTGCA
    GGGCAAGAAGTTGAATTTCAAGGAAAAACATATACAGTTCAAGAATTAACTGATGCTCGT
    GCAAGTGAACATTTCGATTATGTATTAATGAGTGCTGGTGGCGGTACAAGCGAACACTTT
    GCCCCACTTTTTGAAAAAGCTGGTGCAATCGTTATAGACAATTCAAGTCAATGGCGTATG
    GCAGAAGATATTGATTTAATCGTTCCGGAAGTCAATGAACCTACATTTACAAGAGGTATC
    ATTGCCAATCCAAACTGCTCTACGATTCAATCTGTTGTACCTCTAAAAGTATTGCAAGAT
    GCTTATGGTTTAAAACGAGTGGCATATACAACATATCAAGCTGTATCAGGTTCAGGGATG
    AAAGGTAAGAAAGATTTAGCTGAAGGTGTAAATGGTAAAGCACCAGAAGCATATCCACAT
    CCAATTTATAATAATGTGTTACCGCATATTGATGTGTTTTTAGAAAACGGATATACAAAA
    GAAGAACAAAAAATGATTGATGAGACGAGAAAAATTTTAAATGCGCCAGACTTAAAAGTA
    ACAGCAACATGCGCACGTGTGCCTGTTCAAGATAGTCATAGTGTTGAAATTGATGTAACG
    CTTGACAAAGAAACAACAGCAGAAGATATTAAAGCGTTATTTGATCAAGATGACCGCGTT
    GTTTTAGTAGACAATCCAGAGAACAATGAATATCCAATGGCAATCAATTCTACTAATAAA
    GATGAAGTGTTTGTTGGCCGTATACGTAGAGATGATTCATTAGAAAATACTTTCCATGTA
    TGGTGTACATCAGACAATTTATTAAAAGGTGCTGCATTAAATGCTGTACAAGTATTGGAA
    CAAGTTATGCGTTTAAAAGGAGCGAATTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS07350 [old locus tag: NWMN_1305 ]
  • symbol: NWMN_RS07350
  • description: aspartate-semialdehyde dehydrogenase
  • length: 329
  • theoretical pI: 4.64802
  • theoretical MW: 36282.7
  • GRAVY: -0.293009

Function[edit source | edit]

  • reaction:
    EC 1.2.1.11?  ExPASy
    Aspartate-semialdehyde dehydrogenaseL-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 379.1)
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 126.2)
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 83.2)
    MetabolismAmino acid biosynthesisGlutamate familyN-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 58.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxineerythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 30)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisglyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 27.6)
    MetabolismAmino acid biosynthesisGlutamate familyN-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 27.6)
    glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 16.4)
    Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 14.3)
    Genetic information processingMobile and extrachromosomal element functionsPlasmid functionsintegrating conjugative element protein, PFL_4703 family (TIGR03746; HMM-score: 13.6)
  • TheSEED:
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 154.4)
    NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 100)
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 30.2)
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.1)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 14.7)
    no clan definedTGT_C1; C1 domain of tRNA-guanine transglycosylase dimerisation (PF14809; HMM-score: 13.2)
    DUF2895; Protein of unknown function (DUF2895) (PF11444; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.185
    • Ymax_pos: 11
    • Cmax: 0.135
    • Cmax_pos: 24
    • Smax: 0.419
    • Smax_pos: 1
    • Smean: 0.329
    • D: 0.242
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446085676 NCBI
  • RefSeq: WP_000163531 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEVEFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAEDIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGMKGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDLKVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAINSTNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS07350 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]