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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01395
  • pan locus tag?: SAUPAN003807000
  • symbol: SAOUHSC_01395
  • pan gene symbol?: asd
  • synonym:
  • product: aspartate semialdehyde dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01395
  • symbol: SAOUHSC_01395
  • product: aspartate semialdehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1338097..1339086
  • length: 990
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAAAGTTAGCAGTTGTGGGTGCAACAGGATTAGTAGGTACAAAAATGTTGGAGACA
    TTAAATCGTAAAAATATTCCTTTCGATGAATTAGTATTATTTTCATCAGCACGTTCTGCA
    GGGCAAGAAGTTGAATTTCAAGGAAAAACATATACAGTTCAAGAATTAACTGATGCTCGT
    GCAAGTGAACATTTCGATTATGTATTAATGAGTGCTGGTGGCGGTACAAGCGAACACTTT
    GCCCCACTTTTTGAAAAAGCTGGTGCAATCGTTATAGACAATTCAAGTCAATGGCGTATG
    GCAGAAGATATTGATTTAATCGTTCCGGAAGTCAATGAACCTACATTTACAAGAGGTATC
    ATTGCCAATCCAAACTGCTCTACGATTCAATCTGTTGTACCTCTAAAAGTATTGCAAGAT
    GCTTATGGTTTAAAACGAGTGGCATATACAACATATCAAGCTGTATCAGGTTCAGGGATG
    AAAGGTAAGAAAGATTTAGCTGAAGGTGTAAATGGTAAAGCACCAGAAGCATATCCACAT
    CCAATTTATAATAATGTGTTACCGCATATTGATGTGTTTTTAGAAAACGGATATACAAAA
    GAAGAACAAAAAATGATTGATGAGACGAGAAAAATTTTAAATGCGCCAGACTTAAAAGTA
    ACAGCAACATGCGCACGTGTGCCTGTTCAAGATAGTCATAGTGTTGAAATTGATGTAACG
    CTTGACAAAGAAACAACAGCAGAAGATATTAAAGCGTTATTTGATCAAGATGACCGCGTT
    GTTTTAGTAGACAATCCAGAGAACAATGAATATCCAATGGCAATCAATTCTACTAATAAA
    GATGAAGTGTTTGTTGGCCGTATACGTAGAGATGATTCATTAGAAAATACTTTCCATGTA
    TGGTGTACATCAGACAATTTATTAAAAGGTGCTGCATTAAATGCTGTACAAGTATTGGAA
    CAAGTTATGCGTTTAAAAGGAGCGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    990

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01395
  • symbol: SAOUHSC_01395
  • description: aspartate semialdehyde dehydrogenase
  • length: 329
  • theoretical pI: 4.64802
  • theoretical MW: 36282.7
  • GRAVY: -0.293009

Function[edit source | edit]

  • reaction:
    EC 1.2.1.11?  ExPASy
    Aspartate-semialdehyde dehydrogenaseL-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 379.1)
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 126.2)
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 83.2)
    MetabolismAmino acid biosynthesisGlutamate familyN-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 58.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxineerythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 30)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisglyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 27.6)
    MetabolismAmino acid biosynthesisGlutamate familyN-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 27.6)
    glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 16.4)
    Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 14.3)
    Genetic information processingMobile and extrachromosomal element functionsPlasmid functionsintegrating conjugative element protein, PFL_4703 family (TIGR03746; HMM-score: 13.6)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) 
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 154.4)
    NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 100)
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 30.2)
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.1)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 14.7)
    no clan definedTGT_C1; C1 domain of tRNA-guanine transglycosylase dimerisation (PF14809; HMM-score: 13.2)
    DUF2895; Protein of unknown function (DUF2895) (PF11444; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.185
    • Ymax_pos: 11
    • Cmax: 0.135
    • Cmax_pos: 24
    • Smax: 0.419
    • Smax_pos: 1
    • Smean: 0.329
    • D: 0.242
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEVEFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAEDIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGMKGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDLKVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAINSTNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]