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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01400
  • pan locus tag?: SAUPAN003812000
  • symbol: SAOUHSC_01400
  • pan gene symbol?: alr2
  • synonym:
  • product: alanine racemase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01400
  • symbol: SAOUHSC_01400
  • product: alanine racemase
  • replicon: chromosome
  • strand: +
  • coordinates: 1342739..1343824
  • length: 1086
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGACAGCAACATGGTCTGTAAATAAGAAAATATTTTTACAAAATGCAATCACAGTCAAA
    AACAATCAGCCATTAATGGCAGTTGTTAAAAATAATGCATATCACTATGACCTAGAATTT
    GCTGTAACTCAGTTTATCCATGCAGGTATAGATACATTTAGCACAACATCACTACGAGAA
    GCAATTCAAATTAGACAACTTGCTCCAGATGCAACAATCTTTTTAATGAATGCAGTTTAC
    GAGTTTGATTTAGTCCGTGAACATCAAATACACATGACTTTGCCGTCGTTGACATATTAC
    TATAACCATAAAAATGATTTAGCTGGTATTCATGTTCACTTAGAATTTGAAAATTTATTA
    CATCGGTCTGGATTTAAAGATTTAAACGAAATTAAAGAAGTATTGAAAGATCACCATCAT
    AATCAAAATGCAAAAATGATTATTAGTGGTTTATGGACCCATTTTGGATATGCTGATGAA
    TTCGATGTGTCAGATTATAATGTTGAACGTTCACAATGGATGGAAATTGTTGAAGCACTT
    TTATCTGAAGGTTATCAGTTCGACCTAATCCATGCTCAAAATAGTGCGAGTTTTTATCGG
    GAAGGACAAATATTACTACCCCACCATACACATGCGCGTGTAGGTATTGCGTTATACGGT
    TCAAGACCATATAGTTCACTGAATCAACATGATATAGTTCAGTCATTAACTTTAAAAGCA
    CATGTTATTCAAGTGCGCGAAGTACAAGCTGGTGATTATTGCGGTTATAGCTTTGCCTTT
    GAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATT
    TTAAGAACTCGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCA
    TTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACATGCACAAGATGAAGTT
    ATTCTTTATAATAATGATATCCGCATCGATGAATATACCTTTAAAGGTGTTGGTGCAAAT
    TCTGAACAATTAAGTGCTATGAATCATGATTCTTTAAAAAAGGAGTACATTTCAAATGAC
    TGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1086

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01400
  • symbol: SAOUHSC_01400
  • description: alanine racemase
  • length: 361
  • theoretical pI: 6.43166
  • theoretical MW: 41409.5
  • GRAVY: -0.29723

Function[edit source | edit]

  • reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemaseL-alanine = D-alanine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanalanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 148.9)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway Diaminopimelate epimerase alternative form predicted for S.aureus (EC 5.1.1.7) 
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 124.4)
    no clan definedAla_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 70.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.19
    • Ymax_pos: 28
    • Cmax: 0.118
    • Cmax_pos: 28
    • Smax: 0.353
    • Smax_pos: 18
    • Smean: 0.272
    • D: 0.222
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQFIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYNHKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADEFDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIALYGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGYGDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTFKGVGANSEQLSAMNHDSLKKEYISNDC

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 (CodY*)
    CodY* (TF) important in Amino acid metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

M D Wiltshire, S J Foster
Identification and analysis of Staphylococcus aureus components expressed by a model system of growth in serum.
Infect. Immun.: 2001, 69(8);5198-202
[PubMed:11447207] [WorldCat.org] [DOI] (P p)