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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1434 [new locus tag: SACOL_RS07305 ]
  • pan locus tag?: SAUPAN003812000
  • symbol: SACOL1434
  • pan gene symbol?: alr2
  • synonym:
  • product: alanine racemase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1434 [new locus tag: SACOL_RS07305 ]
  • symbol: SACOL1434
  • product: alanine racemase
  • replicon: chromosome
  • strand: +
  • coordinates: 1446512..1447597
  • length: 1086
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGACAGCAACATGGTCTGTAAATAAGAAAATATTTTTACAAAATGCAATCACAGTCAAA
    AACAATCAGCCATTAATGGCAGTTGTTAAAAATAATGCATATCACTATGACCTAGAATTT
    GCTGTAACTCAGTTTATCCATGCAGGTATAGATACATTTAGCACAACATCACTACGAGAA
    GCAATTCAAATTAGACAACTTGCTCCAGATGCAACAATCTTTTTAATGAATGCAGTTTAC
    GAGTTTGATTTAGTCCGTGAACATCAAATACACATGACTTTGCCGTCGTTGACATATTAC
    TATAACCATAAAAATGATTTAGCTGGTATTCATGTTCACTTAGAATTTGAAAATTTATTA
    CATCGGTCTGGATTTAAAGATTTAAACGAAATTAAAGAAGTATTGAAAGATCACCATCAT
    AATCAAAATGCAAAAATGATTATTAGTGGTTTATGGACCCATTTTGGATATGCTGATGAA
    TTCGATGTGTCAGATTATAATGTTGAACGTTCACAATGGATGGAAATTGTTGAAGCACTT
    TTATCTGAAGGTTATCAGTTCGACCTAATCCATGCTCAAAATAGTGCGAGTTTTTATCGG
    GAAGGACAAATATTACTACCCCACCATACACATGCGCGTGTAGGTATTGCGTTATACGGT
    TCAAGACCATATAGTTCACTGAATCAACATGATATAGTTCAGTCATTAACTTTAAAAGCA
    CATGTTATTCAAGTGCGCGAAGTACAAGCTGGTGATTATTGCGGTTATAGCTTTGCCTTT
    GAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATT
    TTAAGAACTCGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCA
    TTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACATGCACAAGATGAAGTT
    ATTCTTTATAATAATGATATCCGCATCGATGAATATACCTTTAAAGGTGTTGGTGCAAAT
    TCTGAACAATTAAGTGCTATGAATCATGATTCTTTAAAAAAGGAGTACATTTCAAATGAC
    TGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1086

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1434 [new locus tag: SACOL_RS07305 ]
  • symbol: SACOL1434
  • description: alanine racemase
  • length: 361
  • theoretical pI: 6.43166
  • theoretical MW: 41409.5
  • GRAVY: -0.29723

Function[edit source | edit]

  • reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemaseL-alanine = D-alanine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanalanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 148.9)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway Diaminopimelate epimerase alternative form predicted for S.aureus (EC 5.1.1.7) 
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 124.4)
    no clan definedAla_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 70.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.19
    • Ymax_pos: 28
    • Cmax: 0.118
    • Cmax_pos: 28
    • Smax: 0.353
    • Smax_pos: 18
    • Smean: 0.272
    • D: 0.222
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQFIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYNHKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADEFDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIALYGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGYGDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTFKGVGANSEQLSAMNHDSLKKEYISNDC

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 190 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]